دورية أكاديمية
Expression of mRNA Encoding the LH Receptor (LHR) and LHR Binding Protein in Granulosa Cells from Nelore (Bos indicus) Heifers Around Follicle Deviation.
| العنوان: | Expression of mRNA Encoding the LH Receptor (LHR) and LHR Binding Protein in Granulosa Cells from Nelore (Bos indicus) Heifers Around Follicle Deviation. |
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| المؤلفون: | Ereno RL; Department of Pharmacology, Institute of Bioscience, University of São Paulo State, Botucatu, São Paulo, Brazil., Loureiro B; Laboratory of Animal Reproductive Physiology, University of Vila Velha (UVV), Vila Velha, Espírito Santo, Brazil., Castilho AC; Department of Pharmacology, Institute of Bioscience, University of São Paulo State, Botucatu, São Paulo, Brazil., Machado MF; Department of Pharmacology, Institute of Bioscience, University of São Paulo State, Botucatu, São Paulo, Brazil., Pegorer MF; Department of Pharmacology, Institute of Bioscience, University of São Paulo State, Botucatu, São Paulo, Brazil., Satrapa RA; Department of Pharmacology, Institute of Bioscience, University of São Paulo State, Botucatu, São Paulo, Brazil., Nogueira MF; Department of Biology Science, University of São Paulo State, Assis, São Paulo, Brazil., Buratini J; Department of Physiology, Institute of Bioscience, University of São Paulo State, Botucatu, São Paulo, Brazil., Barros CM; Department of Pharmacology, Institute of Bioscience, University of São Paulo State, Botucatu, São Paulo, Brazil. |
| المصدر: | Reproduction in domestic animals = Zuchthygiene [Reprod Domest Anim] 2015 Dec; Vol. 50 (6), pp. 952-7. Date of Electronic Publication: 2015 Oct 08. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Paul Parey Scientific Publishers Country of Publication: Germany NLM ID: 9015668 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1439-0531 (Electronic) Linking ISSN: 09366768 NLM ISO Abbreviation: Reprod Domest Anim Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Berlin ; Hamburg : Paul Parey Scientific Publishers, c1990- |
| مواضيع طبية MeSH: | Cattle/*genetics , Granulosa Cells/*metabolism , Ovarian Follicle/*metabolism , Phosphotransferases (Alcohol Group Acceptor)/*genetics , Receptors, LH/*genetics, Animals ; Female ; Gene Expression ; Ovulation/genetics ; RNA, Messenger/genetics ; Signal Transduction/genetics |
| مستخلص: | The time at which follicles acquire LHR in bovine granulosa cells is the subject of some controversy among researchers. The main objective of the present study was to assess the mRNA expression of LHR and LRBP (mRNA protein binding), a post-transcriptional suppressor of LHR mRNA expression, in granulosa cells from the two largest follicles around the expected time of follicle deviation in Nelore heifers. First, the interval between ovulation and follicle deviation in 20 Nelore heifers was determined (2.3 ± 0.2 days after ovulation). Ovulation was hormonally synchronized, and then, heifers were slaughtered on days 2, 2.5 and 3 after ovulation (before, during and after, respectively, the expected time of follicle deviation), and granulosa cells from the two largest follicles were collected. The mRNA abundance of an LHR fragment common to all isoforms (total LHR) and LRBP was assessed by real-time RT-PCR, and LHR alternative transcripts were assessed by semiquantitative RT-PCR followed by electrophoresis. LHR mRNA expression was not detected before the expected time of deviation. Total LHR mRNA abundance was greater in the largest follicle and increased from day 2.5 to 3. In contrast, LRBP mRNA was detected starting on day 2 and was more expressed in the second largest follicle on days 2.5 and 3. The present data suggest that the expression of LHR mRNA in bovine granulosa cells is established after follicle deviation and that the lower abundance of LRBP mRNA after the expected time of deviation may contribute to greater expression of LHR in the bovine dominant follicle. (© 2015 Blackwell Verlag GmbH.) |
| المشرفين على المادة: | 0 (RNA, Messenger) 0 (Receptors, LH) EC 2.7.1.- (Phosphotransferases (Alcohol Group Acceptor)) EC 2.7.1.36 (mevalonate kinase) |
| تواريخ الأحداث: | Date Created: 20151009 Date Completed: 20160818 Latest Revision: 20151109 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1111/rda.12614 |
| PMID: | 26446749 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1439-0531 |
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| DOI: | 10.1111/rda.12614 |