دورية أكاديمية
A Nanoscale Mutation-Sensitive On/Off Switch Based Assays for the Detection of Hepatitis B Virus Lamivudine-Resistant Mutations.
| العنوان: | A Nanoscale Mutation-Sensitive On/Off Switch Based Assays for the Detection of Hepatitis B Virus Lamivudine-Resistant Mutations. |
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| المؤلفون: | Sun A, Pu W, Zhou C, Zhang R, Liu B, Pan Y, Zhang J, Li P, Lil K |
| المصدر: | Journal of nanoscience and nanotechnology [J Nanosci Nanotechnol] 2015 May; Vol. 15 (5), pp. 3939-43. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Scientific Publishers Country of Publication: United States NLM ID: 101088195 Publication Model: Print Cited Medium: Print ISSN: 1533-4880 (Print) Linking ISSN: 15334880 NLM ISO Abbreviation: J Nanosci Nanotechnol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Stevenson Ranch, CA : American Scientific Publishers, 2001-2021 |
| مواضيع طبية MeSH: | Hepatitis B virus*/drug effects , Hepatitis B virus*/genetics , Hepatitis B virus*/isolation & purification, Antiviral Agents/*pharmacology , Drug Resistance, Viral/*genetics , Lamivudine/*pharmacology , Mutation/*genetics, Genotyping Techniques/methods ; Hepatitis B, Chronic/virology ; Humans ; Nanomedicine/methods ; Polymerase Chain Reaction/methods ; Virology/methods |
| مستخلص: | Long-term usage of lamivudine in the treatment of chronic hepatitis B virus (HBV) infection induces the emergence of drug resistance. Sensitive and specific methods aimed at detecting the mutants are clinically useful and required. The purpose of this study was to develop methods for detecting the mutations of YMDD, rtL180M, and rtV173L by nanoscale mutation-sensitive switch consisting of high fidelity polymerase and phosphorothioate-modified allele specific primers. Four assays for these hotspot mutations have been developed with the sensitivity of 100 copies and specificity of at least three log scales for matched templates over mismatched templates. In the condition of multiplex PCR, the sensitivities of these assays are approximately 1000 copies and specificities with two log scales in discrimination of mutant alleles over wild type sequences. These newly developed assays are rapid, accurate, and cost-efficient in detection of lamivudine-related HBV mutants. |
| المشرفين على المادة: | 0 (Antiviral Agents) 2T8Q726O95 (Lamivudine) |
| تواريخ الأحداث: | Date Created: 20151028 Date Completed: 20160201 Latest Revision: 20190715 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1166/jnn.2015.9277 |
| PMID: | 26505028 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1533-4880 |
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| DOI: | 10.1166/jnn.2015.9277 |