دورية أكاديمية
[Influence of Anticoagulants on Detection of ITP Platelet-Specific Autoantibodies and Relationship of Autoantibody Types with Glucocorticoid Efficacy].
| العنوان: | [Influence of Anticoagulants on Detection of ITP Platelet-Specific Autoantibodies and Relationship of Autoantibody Types with Glucocorticoid Efficacy]. |
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| المؤلفون: | Chen Y; Department of Hematology, Nanjing Jiangning Hospital, Nanjing 211101, Jiangsu Province, China., Ge J; Department of Hematology, The First Affiliated Hospital of Anhui Medical University, Hefei 230022, Anhui Province, China., Ruan M; Department of Hematology, The First Affiliated Hospital of Anhui Medical University, Hefei 230022, Anhui Province, China., Zhu LY; Department of Hematology, The First Affiliated Hospital of Anhui Medical University, Hefei 230022, Anhui Province, China., Zeng QS; Department of Hematology, The First Affiliated Hospital of Anhui Medical University, Hefei 230022, Anhui Province, China. E-mail: zengqingshu@medmail.com.cn., Xia RX; Department of Hematology, The First Affiliated Hospital of Anhui Medical University, Hefei 230022, Anhui Province, China., Ni HY; Department of Laboratory Medicine and Pathobiology, University of Toronto, CANADA M2J46A. |
| المصدر: | Zhongguo shi yan xue ye xue za zhi [Zhongguo Shi Yan Xue Ye Xue Za Zhi] 2015 Oct; Vol. 23 (5), pp. 1380-5. |
| نوع المنشور: | Journal Article |
| اللغة: | Chinese |
| بيانات الدورية: | Publisher: Zhongguo shi yan xue za zhi she Country of Publication: China NLM ID: 101084424 Publication Model: Print Cited Medium: Print ISSN: 1009-2137 (Print) Linking ISSN: 10092137 NLM ISO Abbreviation: Zhongguo Shi Yan Xue Ye Xue Za Zhi Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Beijing : Zhongguo shi yan xue za zhi she, |
| مواضيع طبية MeSH: | Anticoagulants/*therapeutic use , Autoantibodies/*blood , Blood Platelets/*immunology , Glucocorticoids/*therapeutic use , Purpura, Thrombocytopenic, Idiopathic/*immunology, Antibodies, Monoclonal ; Antigens, Human Platelet ; Fibrinolytic Agents ; Heparin ; Humans ; Platelet Glycoprotein GPIIb-IIIa Complex ; Purpura, Thrombocytopenic, Idiopathic/blood |
| مستخلص: | Objective: To investigate the influence of divalent cation chelator EDTA and heparin sodium on the detection of ITP platelet-specific autoantibodies by the modified monoclonal antibody immobilization of platelet antigen assay (MAIPA) and to explore the relationship between types of platelet specific autoantibodies and glucocorticoid efficacy. Methods: The samples were obtained from EDTA- and heparin- anticoagulant ITP patients, respectively, so as to detect the platelet-specific autoantibodies (GPIIb/IIIa and GPIbα) in 140 ITP samples by modified MAIPA, then the differences between these two methods were compared. Results: Out of 140 cases in EDTA group, 55 cases were positive for GPIIb/IIIa, while 76 cases in heparin group were positive for GPIIb/IIIa, 42 cases in both group were repeatable; among them 13 cases were positive in EDTA group and negative in heparin group, while 34 cases were positive in heparin group and negative in EDTA group, there was significant difference between them (x (2) = 9.38, P < 0.05), 62 cases in 140 cases of EDTA group were positive for GPIba, while 51 cases in heparin group were positive for GPIba, 42 cases in both group were repeatabe; among them 20 cases were positive in EDTA group and negative in heparin group, while 9 cases were positive in heparin group and negative in EDTA group, there was no significant difference between them (x (2) = 3.44, P > 0.05). A total of 320 cases received a standard glucocorticoid treatment, out of them 143 cases were positive for GPIbα with effective rate 39.9%, 177 cases were negative for GPIbα with effective rate 79.7%, there was statisticalty significant difference between them (x (2) = 53.115, P < 0.05). Conclusion: EDTA anticoagulant (a divalent cation chelator) has a significant influence on detection of ITP platelet-specific autoantibodies (GPIIb/IIIa) by a modified MAIPA method and the GPIbα antibody positive may be one of the important factors that results in un-sensitivity of ITP patients to glucocorticoid treatment. |
| المشرفين على المادة: | 0 (Antibodies, Monoclonal) 0 (Anticoagulants) 0 (Antigens, Human Platelet) 0 (Autoantibodies) 0 (Fibrinolytic Agents) 0 (Glucocorticoids) 0 (Platelet Glycoprotein GPIIb-IIIa Complex) 9005-49-6 (Heparin) |
| تواريخ الأحداث: | Date Created: 20151103 Date Completed: 20160125 Latest Revision: 20181202 |
| رمز التحديث: | 20221213 |
| DOI: | 10.7534/j.issn.1009-2137.2015.05.030 |
| PMID: | 26524042 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1009-2137 |
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| DOI: | 10.7534/j.issn.1009-2137.2015.05.030 |