دورية أكاديمية
Spatial and temporal translational control of germ cell mRNAs mediated by the eIF4E isoform IFE-1.
| العنوان: | Spatial and temporal translational control of germ cell mRNAs mediated by the eIF4E isoform IFE-1. |
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| المؤلفون: | Friday AJ; Department of Biochemistry and Molecular Biology, Brody School of Medicine at East Carolina University, Greenville, NC 27834, USA., Henderson MA; Department of Molecular Sciences, DeBusk College of Osteopathic Medicine, Lincoln Memorial University, Harrogate, TN 37752, USA., Morrison JK; Department of Biochemistry and Molecular Biology, Brody School of Medicine at East Carolina University, Greenville, NC 27834, USA., Hoffman JL; Department of Biochemistry and Molecular Biology, Brody School of Medicine at East Carolina University, Greenville, NC 27834, USA., Keiper BD; Department of Biochemistry and Molecular Biology, Brody School of Medicine at East Carolina University, Greenville, NC 27834, USA keiperb@ecu.edu. |
| المصدر: | Journal of cell science [J Cell Sci] 2015 Dec 15; Vol. 128 (24), pp. 4487-98. Date of Electronic Publication: 2015 Nov 05. |
| نوع المنشور: | Journal Article; Research Support, U.S. Gov't, Non-P.H.S. |
| اللغة: | English |
| بيانات الدورية: | Publisher: Company of Biologists Country of Publication: England NLM ID: 0052457 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1477-9137 (Electronic) Linking ISSN: 00219533 NLM ISO Abbreviation: J Cell Sci Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Cambridge : Company of Biologists Original Publication: London. |
| مواضيع طبية MeSH: | Caenorhabditis elegans/*metabolism , Caenorhabditis elegans Proteins/*metabolism , Eukaryotic Initiation Factors/*metabolism , Oocytes/*metabolism , Protein Biosynthesis/*physiology , RNA, Helminth/*metabolism , RNA, Messenger/*metabolism, Animals ; Caenorhabditis elegans/genetics ; Caenorhabditis elegans Proteins/genetics ; Eukaryotic Initiation Factor-4E/genetics ; Eukaryotic Initiation Factor-4E/metabolism ; Eukaryotic Initiation Factors/genetics ; Extracellular Signal-Regulated MAP Kinases/genetics ; Extracellular Signal-Regulated MAP Kinases/metabolism ; Female ; MAP Kinase Signaling System/physiology ; Oocytes/cytology ; Polyribosomes/genetics ; Polyribosomes/metabolism ; RNA, Helminth/genetics ; RNA, Messenger/genetics |
| مستخلص: | Regulated mRNA translation is vital for germ cells to produce new proteins in the spatial and temporal patterns that drive gamete development. Translational control involves the de-repression of stored mRNAs and their recruitment by eukaryotic initiation factors (eIFs) to ribosomes. C. elegans expresses five eIF4Es (IFE-1-IFE-5); several have been shown to selectively recruit unique pools of mRNA. Individual IFE knockouts yield unique phenotypes due to inefficient translation of certain mRNAs. Here, we identified mRNAs preferentially translated through the germline-specific eIF4E isoform IFE-1. Differential polysome microarray analysis identified 77 mRNAs recruited by IFE-1. Among the IFE-1-dependent mRNAs are several required for late germ cell differentiation and maturation. Polysome association of gld-1, vab-1, vpr-1, rab-7 and rnp-3 mRNAs relies on IFE-1. Live animal imaging showed IFE-1-dependent selectivity in spatial and temporal translation of germline mRNAs. Altered MAPK activation in oocytes suggests dual roles for IFE-1, both promoting and suppressing oocyte maturation at different stages. This single eIF4E isoform exerts positive, selective translational control during germ cell differentiation. (© 2015. Published by The Company of Biologists Ltd.) |
| فهرسة مساهمة: | Keywords: C. elegans; Meiotic maturation; Oogenesis; Protein synthesis; TSAA; Translation state array analysis; mRNA translational control |
| المشرفين على المادة: | 0 (Caenorhabditis elegans Proteins) 0 (Eukaryotic Initiation Factor-4E) 0 (Eukaryotic Initiation Factors) 0 (IFE-1 protein, C elegans) 0 (RNA, Helminth) 0 (RNA, Messenger) EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases) |
| تواريخ الأحداث: | Date Created: 20151107 Date Completed: 20160921 Latest Revision: 20151216 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1242/jcs.172684 |
| PMID: | 26542024 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1477-9137 |
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| DOI: | 10.1242/jcs.172684 |