دورية أكاديمية
أعرض في EDS

Neuroprotection and immunomodulation by xenografted human mesenchymal stem cells following spinal cord ventral root avulsion.

التفاصيل البيبلوغرافية
العنوان: Neuroprotection and immunomodulation by xenografted human mesenchymal stem cells following spinal cord ventral root avulsion.
المؤلفون: Ribeiro TB; Hematology and Hemotherapy Center-University of Campinas/Hemocentro-Unicamp, Instituto Nacional de Ciência e Tecnologia do Sangue, Campinas, São Paulo, Brazil., Duarte AS; Hematology and Hemotherapy Center-University of Campinas/Hemocentro-Unicamp, Instituto Nacional de Ciência e Tecnologia do Sangue, Campinas, São Paulo, Brazil., Longhini AL; Hematology and Hemotherapy Center-University of Campinas/Hemocentro-Unicamp, Instituto Nacional de Ciência e Tecnologia do Sangue, Campinas, São Paulo, Brazil.; Neuroimmunomodulation Group, Dept. Genetics, Evolution and Bioagents, University of Campinas, Campinas, Brazil., Pradella F; Neuroimmunomodulation Group, Dept. Genetics, Evolution and Bioagents, University of Campinas, Campinas, Brazil., Farias AS; Neuroimmunomodulation Group, Dept. Genetics, Evolution and Bioagents, University of Campinas, Campinas, Brazil., Luzo AC; Hematology and Hemotherapy Center-University of Campinas/Hemocentro-Unicamp, Instituto Nacional de Ciência e Tecnologia do Sangue, Campinas, São Paulo, Brazil., Oliveira AL; Dept. of Structural and Functional Biology, Institute of Biology, University of Campinas, Campinas, Brazil., Olalla Saad ST; Hematology and Hemotherapy Center-University of Campinas/Hemocentro-Unicamp, Instituto Nacional de Ciência e Tecnologia do Sangue, Campinas, São Paulo, Brazil.
المصدر: Scientific reports [Sci Rep] 2015 Nov 09; Vol. 5, pp. 16167. Date of Electronic Publication: 2015 Nov 09.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : Nature Publishing Group, copyright 2011-
مواضيع طبية MeSH: Mesenchymal Stem Cell Transplantation*, Motor Neurons/*pathology , Radiculopathy/*therapy , Spinal Cord/*transplantation, Adipose Tissue/cytology ; Adipose Tissue/transplantation ; Animals ; Heterografts ; Humans ; Immunomodulation ; Mesenchymal Stem Cells/cytology ; Mesenchymal Stem Cells/metabolism ; Nerve Regeneration ; Neuroprotection ; Radiculopathy/immunology ; Radiculopathy/metabolism ; Radiculopathy/pathology ; Rats ; Spinal Cord/physiopathology ; Spinal Nerve Roots/physiopathology ; Synapses/immunology ; Synapses/metabolism ; Synapses/pathology ; Synaptophysin/metabolism ; T-Lymphocytes/immunology
مستخلص: The present study investigates the effects of xenotransplantation of Adipose Tissue Mesenchymal Stem Cells (AT-MSCs) in animals after ventral root avulsion. AT-MSC has similar characteristics to bone marrow mesenchymal stem cells (BM-MSCs), such as immunomodulatory properties and expression of neurotrophic factors. In this study, Lewis rats were submitted to surgery for unilateral avulsion of the lumbar ventral roots and received 5 × 10(5) AT-MSCs via the lateral funiculus. Two weeks after cell administration, the animals were sacrificed and the moto neurons, T lymphocytes and cell defense nervous system were analyzed. An increased neuronal survival and partial preservation of synaptophysin-positive nerve terminals, related to GDNF and BDNF expression of AT-MSCs, and reduction of pro-inflammatory reaction were observed. In conclusion, AT-MSCs prevent second phase neuronal injury, since they suppressed lymphocyte, astroglia and microglia effects, which finally contributed to rat motor-neuron survival and synaptic stability of the lesioned motor-neuron. Moreover, the survival of the injected AT- MSCs lasted for at least 14 days. These results indicate that neuronal survival after lesion, followed by mesenchymal stem cell (MSC) administration, might occur through cytokine release and immunomodulation, thus suggesting that AT-MSCs are promising cells for the therapy of neuronal lesions.
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المشرفين على المادة: 0 (Synaptophysin)
تواريخ الأحداث: Date Created: 20151110 Date Completed: 20160907 Latest Revision: 20240521
رمز التحديث: 20240521
مُعرف محوري في PubMed: PMC4637826
DOI: 10.1038/srep16167
PMID: 26548646
قاعدة البيانات: MEDLINE
الوصف
تدمد:2045-2322
DOI:10.1038/srep16167