دورية أكاديمية
Late cornified envelope (LCE) proteins: distinct expression patterns of LCE2 and LCE3 members suggest nonredundant roles in human epidermis and other epithelia.
| العنوان: | Late cornified envelope (LCE) proteins: distinct expression patterns of LCE2 and LCE3 members suggest nonredundant roles in human epidermis and other epithelia. |
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| المؤلفون: | Niehues H; Department of Dermatology, Radboud University Medical Center, Radboud Institute for Molecular Life Sciences, PO Box 9101, 6500 HB, Nijmegen, the Netherlands., van Vlijmen-Willems IM; Department of Dermatology, Radboud University Medical Center, Radboud Institute for Molecular Life Sciences, PO Box 9101, 6500 HB, Nijmegen, the Netherlands., Bergboer JG; Massachusetts General Hospital/Harvard Medical School, Boston, MA, U.S.A., Kersten FF; Department of Dermatology, Radboud University Medical Center, Radboud Institute for Molecular Life Sciences, PO Box 9101, 6500 HB, Nijmegen, the Netherlands., Narita M; Cancer Research U.K. Cambridge Institute, University of Cambridge, The Li Ka Shing Centre, Cambridge, U.K., Hendriks WJ; Department of Cell Biology, Radboud University Medical Center, Radboud Institute for Molecular Life Sciences, PO Box 9101, 6500 HB, Nijmegen, the Netherlands., van den Bogaard EH; Department of Dermatology, Radboud University Medical Center, Radboud Institute for Molecular Life Sciences, PO Box 9101, 6500 HB, Nijmegen, the Netherlands., Zeeuwen PL; Department of Dermatology, Radboud University Medical Center, Radboud Institute for Molecular Life Sciences, PO Box 9101, 6500 HB, Nijmegen, the Netherlands., Schalkwijk J; Department of Dermatology, Radboud University Medical Center, Radboud Institute for Molecular Life Sciences, PO Box 9101, 6500 HB, Nijmegen, the Netherlands. |
| المصدر: | The British journal of dermatology [Br J Dermatol] 2016 Apr; Vol. 174 (4), pp. 795-802. Date of Electronic Publication: 2016 Jan 03. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Oxford University Press Country of Publication: England NLM ID: 0004041 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1365-2133 (Electronic) Linking ISSN: 00070963 NLM ISO Abbreviation: Br J Dermatol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2023- : Oxford : Oxford University Press Original Publication: Oxford [etc.] Published for the British Association of Dermatologists by Blackwell Scientific Publications [etc.] |
| مواضيع طبية MeSH: | Cornified Envelope Proline-Rich Proteins/*metabolism , Epidermis/*metabolism, Adolescent ; Adult ; Aged ; Cells, Cultured ; Humans ; Keratinocytes/metabolism ; Middle Aged ; Models, Biological ; Mouth Mucosa/metabolism ; Psoriasis/metabolism ; Young Adult |
| مستخلص: | Background: Deletion of the late cornified envelope (LCE) proteins LCE3B and LCE3C is a strong and widely replicated psoriasis risk factor. It is amenable to biological analysis because it precludes the expression of two epidermis-specific proteins, rather than being a single-nucleotide polymorphism of uncertain significance. The biology of the 18-member LCE family of highly homologous proteins has remained largely unexplored so far. Objectives: To analyse LCE3 expression at the protein level in human epithelia, as a starting point for functional analyses of these proteins in health and disease. Methods: We generated the first pan-LCE3 monoclonal antibody and provide a detailed analysis of its specificity towards individual LCE members. LCE2 and LCE3 expression in human tissues and in reconstructed human skin models was studied using immunohistochemical analyses and quantitative polymerase chain reaction. Results: Our study reveals that LCE2 and LCE3 proteins are differentially expressed in human epidermis, and colocalize only in the upper stratum granulosum layer. Using an in vitro reconstructed human skin model that mimics epidermal morphogenesis, we found that LCE3 proteins are expressed at an early time point during epidermal differentiation in the suprabasal layers, while LCE2 proteins are found only in the uppermost granular layer and stratum corneum. Conclusions: Based on the localization of LCE2 and LCE3 in human epidermis we conclude that members of the LCE protein family are likely to have distinct functions in epidermal biology. This finding may contribute to understanding why LCE3B/C deletion increases psoriasis risk. (© 2015 British Association of Dermatologists.) |
| معلومات مُعتمدة: | 15890 United Kingdom CRUK_ Cancer Research UK |
| المشرفين على المادة: | 0 (Cornified Envelope Proline-Rich Proteins) |
| تواريخ الأحداث: | Date Created: 20151112 Date Completed: 20170306 Latest Revision: 20240210 |
| رمز التحديث: | 20240210 |
| DOI: | 10.1111/bjd.14284 |
| PMID: | 26556599 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1365-2133 |
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| DOI: | 10.1111/bjd.14284 |