دورية أكاديمية
أعرض في EDS

Structural basis for RNA-genome recognition during bacteriophage Qβ replication.

التفاصيل البيبلوغرافية
العنوان: Structural basis for RNA-genome recognition during bacteriophage Qβ replication.
المؤلفون: Gytz H; Department of Molecular Biology and Genetics, Aarhus University, DK-8000 Aarhus C, Denmark., Mohr D; Department of Molecular Biology and Genetics, Aarhus University, DK-8000 Aarhus C, Denmark., Seweryn P; Department of Molecular Biology and Genetics, Aarhus University, DK-8000 Aarhus C, Denmark., Yoshimura Y; Interdisciplinary Nanoscience Centre (iNANO) and Department of Chemistry, Aarhus University, DK-8000 Aarhus C, Denmark., Kutlubaeva Z; Institute of Protein Research of the Russian Academy of Sciences, Pushchino, Moscow Region 142290, Russia., Dolman F; Department of Molecular Biology and Genetics, Aarhus University, DK-8000 Aarhus C, Denmark., Chelchessa B; Department of Molecular Biology and Genetics, Aarhus University, DK-8000 Aarhus C, Denmark., Chetverin AB; Institute of Protein Research of the Russian Academy of Sciences, Pushchino, Moscow Region 142290, Russia., Mulder FA; Interdisciplinary Nanoscience Centre (iNANO) and Department of Chemistry, Aarhus University, DK-8000 Aarhus C, Denmark., Brodersen DE; Department of Molecular Biology and Genetics, Aarhus University, DK-8000 Aarhus C, Denmark., Knudsen CR; Department of Molecular Biology and Genetics, Aarhus University, DK-8000 Aarhus C, Denmark crk@mbg.au.dk.
المصدر: Nucleic acids research [Nucleic Acids Res] 2015 Dec 15; Vol. 43 (22), pp. 10893-906. Date of Electronic Publication: 2015 Nov 17.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Oxford University Press Country of Publication: England NLM ID: 0411011 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1362-4962 (Electronic) Linking ISSN: 03051048 NLM ISO Abbreviation: Nucleic Acids Res Subsets: MEDLINE
أسماء مطبوعة: Publication: 1992- : Oxford : Oxford University Press
Original Publication: London, Information Retrieval ltd.
مواضيع طبية MeSH: Genome, Viral* , Virus Replication*, Allolevivirus/*physiology , Q beta Replicase/*chemistry, Allolevivirus/genetics ; Dimerization ; Escherichia coli Proteins/chemistry ; Escherichia coli Proteins/metabolism ; Mutation ; Protein Binding ; Protein Structure, Tertiary ; Protein Subunits/chemistry ; Protein Subunits/genetics ; Protein Subunits/metabolism ; Q beta Replicase/genetics ; Q beta Replicase/metabolism ; RNA, Viral/biosynthesis ; RNA, Viral/metabolism ; Ribosomal Proteins/chemistry ; Ribosomal Proteins/metabolism
مستخلص: Upon infection of Escherichia coli by bacteriophage Qβ, the virus-encoded β-subunit recruits host translation elongation factors EF-Tu and EF-Ts and ribosomal protein S1 to form the Qβ replicase holoenzyme complex, which is responsible for amplifying the Qβ (+)-RNA genome. Here, we use X-ray crystallography, NMR spectroscopy, as well as sequence conservation, surface electrostatic potential and mutational analyses to decipher the roles of the β-subunit and the first two oligonucleotide-oligosaccharide-binding domains of S1 (OB1-2) in the recognition of Qβ (+)-RNA by the Qβ replicase complex. We show how three basic residues of the β subunit form a patch located adjacent to the OB2 domain, and use NMR spectroscopy to demonstrate for the first time that OB2 is able to interact with RNA. Neutralization of the basic residues by mutagenesis results in a loss of both the phage infectivity in vivo and the ability of Qβ replicase to amplify the genomic RNA in vitro. In contrast, replication of smaller replicable RNAs is not affected. Taken together, our data suggest that the β-subunit and protein S1 cooperatively bind the (+)-stranded Qβ genome during replication initiation and provide a foundation for understanding template discrimination during replication initiation.
(© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.)
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سلسلة جزيئية: PDB 4R71
المشرفين على المادة: 0 (Escherichia coli Proteins)
0 (Protein Subunits)
0 (RNA, Viral)
0 (Ribosomal Proteins)
0 (ribosomal protein S1, E coli)
EC 2.7.7.- (Q beta Replicase)
تواريخ الأحداث: Date Created: 20151119 Date Completed: 20160621 Latest Revision: 20181113
رمز التحديث: 20240829
مُعرف محوري في PubMed: PMC4678825
DOI: 10.1093/nar/gkv1212
PMID: 26578560
قاعدة البيانات: MEDLINE
الوصف
تدمد:1362-4962
DOI:10.1093/nar/gkv1212