دورية أكاديمية
أعرض في EDS

Perinuclear Anchoring of H3K9-Methylated Chromatin Stabilizes Induced Cell Fate in C. elegans Embryos.

التفاصيل البيبلوغرافية
العنوان: Perinuclear Anchoring of H3K9-Methylated Chromatin Stabilizes Induced Cell Fate in C. elegans Embryos.
المؤلفون: Gonzalez-Sandoval A; Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, CH-4058 Basel, Switzerland; Faculty of Natural Sciences, University of Basel, Klingelbergstrasse 50/70, CH-4056 Basel, Switzerland., Towbin BD; Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, CH-4058 Basel, Switzerland., Kalck V; Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, CH-4058 Basel, Switzerland., Cabianca DS; Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, CH-4058 Basel, Switzerland., Gaidatzis D; Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, CH-4058 Basel, Switzerland; Swiss Institute of Bioinformatics, CH-4058 Basel, Switzerland., Hauer MH; Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, CH-4058 Basel, Switzerland; Faculty of Natural Sciences, University of Basel, Klingelbergstrasse 50/70, CH-4056 Basel, Switzerland., Geng L; China Novartis Institute of Biomedical Research Co, Ltd. Bldg 3, Lane 3728 Jinke Road, Pudong New Area, Shanghai 201203, China., Wang L; China Novartis Institute of Biomedical Research Co, Ltd. Bldg 3, Lane 3728 Jinke Road, Pudong New Area, Shanghai 201203, China., Yang T; China Novartis Institute of Biomedical Research Co, Ltd. Bldg 3, Lane 3728 Jinke Road, Pudong New Area, Shanghai 201203, China., Wang X; China Novartis Institute of Biomedical Research Co, Ltd. Bldg 3, Lane 3728 Jinke Road, Pudong New Area, Shanghai 201203, China., Zhao K; China Novartis Institute of Biomedical Research Co, Ltd. Bldg 3, Lane 3728 Jinke Road, Pudong New Area, Shanghai 201203, China., Gasser SM; Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, CH-4058 Basel, Switzerland; Faculty of Natural Sciences, University of Basel, Klingelbergstrasse 50/70, CH-4056 Basel, Switzerland. Electronic address: susan.gasser@fmi.ch.
المصدر: Cell [Cell] 2015 Dec 03; Vol. 163 (6), pp. 1333-47. Date of Electronic Publication: 2015 Nov 19.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Cell Press Country of Publication: United States NLM ID: 0413066 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1097-4172 (Electronic) Linking ISSN: 00928674 NLM ISO Abbreviation: Cell Subsets: MEDLINE
أسماء مطبوعة: Publication: Cambridge, Ma : Cell Press
Original Publication: Cambridge, MIT Press.
مواضيع طبية MeSH: Heterochromatin* , Histone Code*, Caenorhabditis elegans/*embryology , Caenorhabditis elegans/*genetics , Caenorhabditis elegans Proteins/*metabolism , Chromosomal Proteins, Non-Histone/*metabolism , Embryo, Nonmammalian/*cytology, Amino Acid Sequence ; Animals ; Caenorhabditis elegans/cytology ; Caenorhabditis elegans/metabolism ; Caenorhabditis elegans Proteins/chemistry ; Caenorhabditis elegans Proteins/genetics ; Cell Differentiation ; Cell Nucleus/genetics ; Cell Nucleus/metabolism ; Chromosomal Proteins, Non-Histone/chemistry ; Chromosomal Proteins, Non-Histone/genetics ; Embryo, Nonmammalian/metabolism ; Molecular Sequence Data ; Sequence Alignment
مستخلص: Interphase chromatin is organized in distinct nuclear sub-compartments, reflecting its degree of compaction and transcriptional status. In Caenorhabditis elegans embryos, H3K9 methylation is necessary to silence and to anchor repeat-rich heterochromatin at the nuclear periphery. In a screen for perinuclear anchors of heterochromatin, we identified a previously uncharacterized C. elegans chromodomain protein, CEC-4. CEC-4 binds preferentially mono-, di-, or tri-methylated H3K9 and localizes at the nuclear envelope independently of H3K9 methylation and nuclear lamin. CEC-4 is necessary for endogenous heterochromatin anchoring, but not for transcriptional repression, in contrast to other known H3K9 methyl-binders in worms, which mediate gene repression but not perinuclear anchoring. When we ectopically induce a muscle differentiation program in embryos, cec-4 mutants fail to commit fully to muscle cell fate. This suggests that perinuclear sequestration of chromatin during development helps restrict cell differentiation programs by stabilizing commitment to a specific cell fate. PAPERCLIP.
(Copyright © 2015 Elsevier Inc. All rights reserved.)
التعليقات: Comment in: Dev Cell. 2015 Dec 21;35(6):670-1. (PMID: 26702826)
Comment in: Worm. 2016 May 31;5(3):e1190900. (PMID: 27695653)
سلسلة جزيئية: GEO GSE74134
المشرفين على المادة: 0 (CEC-4 protein, C elegans)
0 (Caenorhabditis elegans Proteins)
0 (Chromosomal Proteins, Non-Histone)
0 (Heterochromatin)
تواريخ الأحداث: Date Created: 20151127 Date Completed: 20160405 Latest Revision: 20161003
رمز التحديث: 20240628
DOI: 10.1016/j.cell.2015.10.066
PMID: 26607792
قاعدة البيانات: MEDLINE
الوصف
تدمد:1097-4172
DOI:10.1016/j.cell.2015.10.066