دورية أكاديمية
Investigation of a staphylococcal food poisoning outbreak combining case-control, traditional typing and whole genome sequencing methods, Luxembourg, June 2014.
| العنوان: | Investigation of a staphylococcal food poisoning outbreak combining case-control, traditional typing and whole genome sequencing methods, Luxembourg, June 2014. |
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| المؤلفون: | Mossong J; Laboratoire National de Santé, Dudelange, Luxembourg., Decruyenaere F, Moris G, Ragimbeau C, Olinger CM, Johler S, Perrin M, Hau P, Weicherding P |
| المصدر: | Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin [Euro Surveill] 2015; Vol. 20 (45). |
| نوع المنشور: | Case Reports; Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: European Centre for Disease Prevention and Control (ECDC) Country of Publication: Sweden NLM ID: 100887452 Publication Model: Print Cited Medium: Internet ISSN: 1560-7917 (Electronic) Linking ISSN: 1025496X NLM ISO Abbreviation: Euro Surveill Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Stockholm, Sweden : European Centre for Disease Prevention and Control (ECDC) Original Publication: Saint-Maurice : Hôpital national de Saint-Maurice |
| مواضيع طبية MeSH: | Disease Outbreaks*, Enterotoxins/*genetics , Staphylococcal Food Poisoning/*epidemiology , Staphylococcal Infections/*epidemiology , Staphylococcus aureus/*genetics, Adult ; Case-Control Studies ; Female ; Food Microbiology ; Genome, Bacterial ; Genotype ; Humans ; Luxembourg/epidemiology ; Male ; Molecular Typing ; Phylogeny ; Sequence Analysis ; Staphylococcal Food Poisoning/diagnosis ; Staphylococcal Food Poisoning/microbiology ; Staphylococcus aureus/isolation & purification |
| مستخلص: | In June 2014, a staphylococcal food poisoning outbreak occurred at an international equine sports event in Luxembourg requiring the hospitalisation of 31 persons. We conducted a microbiological investigation of patients and buffet items, a case-control study and a carriage study of catering staff. Isolates of Staphylococcus aureus from patients, food and catering staff were characterised and compared using traditional typing methods and whole genome sequencing. Genotypically identical strains (sequence type ST8, spa-type t024, MLVA-type 4698, enterotoxin A FRI100) were isolated in 10 patients, shiitake mushrooms, cured ham, and in three members of staff. The case-control study strongly suggested pasta salad with pesto as the vehicle of infection (p<0.001), but this food item could not be tested, because there were no leftovers. Additional enterotoxigenic strains genetically unrelated to the outbreak strain were found in four members of staff. Non-enterotoxigenic strains with livestock-associated sequence type ST398 were isolated from three food items and two members of staff. The main cause of the outbreak is likely to have been not maintaining the cold chain after food preparation. Whole genome sequencing resulted in phylogenetic clustering which concurred with traditional typing while simultaneously characterising virulence and resistance traits. |
| فهرسة مساهمة: | Keywords: Luxembourg; Staphylococcus aureus; Whole genome sequencing; case–control study; colonisation; food poisoning |
| المشرفين على المادة: | 0 (Enterotoxins) |
| تواريخ الأحداث: | Date Created: 20151127 Date Completed: 20160418 Latest Revision: 20151126 |
| رمز التحديث: | 20231215 |
| DOI: | 10.2807/1560-7917.ES.2015.20.45.30059 |
| PMID: | 26608881 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1560-7917 |
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| DOI: | 10.2807/1560-7917.ES.2015.20.45.30059 |