دورية أكاديمية
أعرض في EDS

RNA-seq de novo Assembly Reveals Differential Gene Expression in Glossina palpalis gambiensis Infected with Trypanosoma brucei gambiense vs. Non-Infected and Self-Cured Flies.

التفاصيل البيبلوغرافية
العنوان: RNA-seq de novo Assembly Reveals Differential Gene Expression in Glossina palpalis gambiensis Infected with Trypanosoma brucei gambiense vs. Non-Infected and Self-Cured Flies.
المؤلفون: Hamidou Soumana I; UMR 177, Institut de Recherche Pour le Développement-CIRAD, CIRAD TA A-17/G Montpellier, France., Klopp C; Institut National de la Recherche Agronomique, GenoToul, UR875 Castanet-Tolosan, France., Ravel S; UMR 177, Institut de Recherche Pour le Développement-CIRAD, CIRAD TA A-17/G Montpellier, France., Nabihoudine I; Institut National de la Recherche Agronomique, GenoToul, UR875 Castanet-Tolosan, France., Tchicaya B; UMR 177, Institut de Recherche Pour le Développement-CIRAD, CIRAD TA A-17/G Montpellier, France., Parrinello H; Centre National de la Recherche Scientifique, Unité Mixte de Recherche 5203, Institut de Génomique Fonctionnelle Montpellier, France ; Institut National de la Santé et de la Recherche Médicale U661 Montpellier, France ; Universités de Montpellier 1 and 2, UMR 5203 Montpellier, France ; Montpellier GenomiX, Institut de Génomique Fonctionnelle Montpellier, France., Abate L; UMR MIVEGEC (Institut de Recherche pour le Développement 224-Centre National de la Recherche Scientifique 5290-UM1-UM2), Institut de Recherche pour le Développement Montpellier, France., Rialle S; Centre National de la Recherche Scientifique, Unité Mixte de Recherche 5203, Institut de Génomique Fonctionnelle Montpellier, France ; Institut National de la Santé et de la Recherche Médicale U661 Montpellier, France ; Universités de Montpellier 1 and 2, UMR 5203 Montpellier, France ; Montpellier GenomiX, Institut de Génomique Fonctionnelle Montpellier, France., Geiger A; UMR 177, Institut de Recherche Pour le Développement-CIRAD, CIRAD TA A-17/G Montpellier, France.
المصدر: Frontiers in microbiology [Front Microbiol] 2015 Nov 13; Vol. 6, pp. 1259. Date of Electronic Publication: 2015 Nov 13 (Print Publication: 2015).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Frontiers Research Foundation Country of Publication: Switzerland NLM ID: 101548977 Publication Model: eCollection Cited Medium: Print ISSN: 1664-302X (Print) Linking ISSN: 1664302X NLM ISO Abbreviation: Front Microbiol Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Lausanne : Frontiers Research Foundation
مستخلص: Trypanosoma brucei gambiense (Tbg), causing the sleeping sickness chronic form, completes its developmental cycle within the tsetse fly vector Glossina palpalis gambiensis (Gpg) before its transmission to humans. Within the framework of an anti-vector disease control strategy, a global gene expression profiling of trypanosome infected (susceptible), non-infected, and self-cured (refractory) tsetse flies was performed, on their midguts, to determine differential genes expression resulting from in vivo trypanosomes, tsetse flies (and their microbiome) interactions. An RNAseq de novo assembly was achieved. The assembled transcripts were mapped to reference sequences for functional annotation. Twenty-four percent of the 16,936 contigs could not be annotated, possibly representing untranslated mRNA regions, or Gpg- or Tbg-specific ORFs. The remaining contigs were classified into 65 functional groups. Only a few transposable elements were present in the Gpg midgut transcriptome, which may represent active transpositions and play regulatory roles. One thousand three hundred and seventy three genes differentially expressed (DEGs) between stimulated and non-stimulated flies were identified at day-3 post-feeding; 52 and 1025 between infected and self-cured flies at 10 and 20 days post-feeding, respectively. The possible roles of several DEGs regarding fly susceptibility and refractoriness are discussed. The results provide new means to decipher fly infection mechanisms, crucial to develop anti-vector control strategies.
References: Insect Biochem Mol Biol. 2003 Nov;33(11):1155-64. (PMID: 14563366)
Nat Rev Genet. 2009 Jan;10(1):57-63. (PMID: 19015660)
Thromb Haemost. 1979 Aug 31;42(2):743-51. (PMID: 505376)
Lancet. 2009 May 2;373(9674):1570-5. (PMID: 19410718)
J Proteomics. 2011 Aug 24;74(9):1625-43. (PMID: 21316496)
J Biol Chem. 1998 Aug 14;273(33):20802-9. (PMID: 9694825)
Curr Issues Mol Biol. 2004 Jan;6(1):57-71. (PMID: 14632259)
Front Microbiol. 2014 Nov 10;5:609. (PMID: 25426112)
Science. 2014 Apr 25;344(6182):380-6. (PMID: 24763584)
Ann Neurol. 2008 Aug;64(2):116-26. (PMID: 18756506)
Dev Comp Immunol. 1999 Jun-Jul;23(4-5):291-301. (PMID: 10426423)
Bioinformatics. 2012 Apr 15;28(8):1086-92. (PMID: 22368243)
Annu Rev Entomol. 1997;42:525-50. (PMID: 15012322)
PLoS One. 2007 Feb 21;2(2):e239. (PMID: 17318257)
PLoS Med. 2008 Feb;5(2):e55. (PMID: 18303943)
J Invertebr Pathol. 2013 Mar;112 Suppl:S89-93. (PMID: 22841948)
Genome Res. 2008 May;18(5):821-9. (PMID: 18349386)
Genome Biol. 2010;11(10):R106. (PMID: 20979621)
Proc Natl Acad Sci U S A. 1998 Nov 24;95(24):14290-5. (PMID: 9826693)
Nat Methods. 2014 Aug;11(8):817-20. (PMID: 25042786)
Trends Microbiol. 2004 Jul;12(7):325-36. (PMID: 15223060)
Annu Rev Entomol. 2002;47:535-59. (PMID: 11729084)
Bioinformatics. 2006 Jul 1;22(13):1658-9. (PMID: 16731699)
Immunol Rev. 2004 Apr;198:97-105. (PMID: 15199957)
J Invertebr Pathol. 2013 Mar;112 Suppl:S2-10. (PMID: 22841636)
Parasitology. 1999 May;118 ( Pt 5):469-78. (PMID: 10363280)
J Med Entomol. 2007 Jul;44(4):660-5. (PMID: 17695022)
Infect Genet Evol. 2014 Jun;24:41-56. (PMID: 24637266)
Insect Biochem Mol Biol. 2006 Apr;36(4):344-52. (PMID: 16551548)
Kinetoplastid Biol Dis. 2003 Apr 10;2(1):1. (PMID: 12769824)
Nat Biotechnol. 2010 May;28(5):511-5. (PMID: 20436464)
BMC Genomics. 2010 Mar 30;11:213. (PMID: 20353571)
Front Microbiol. 2014 Nov 17;5:620. (PMID: 25452752)
Clin Epidemiol. 2014 Aug 06;6:257-75. (PMID: 25125985)
Exp Parasitol. 1994 Sep;79(2):202-5. (PMID: 8056082)
Front Cell Infect Microbiol. 2013 Jul 24;3:34. (PMID: 23898466)
Adv Exp Med Biol. 2008;627:35-48. (PMID: 18510012)
Nat Protoc. 2013 Aug;8(8):1494-512. (PMID: 23845962)
Infect Genet Evol. 2010 Jan;10(1):115-21. (PMID: 19879380)
J Econ Entomol. 2000 Feb;93(1):123-35. (PMID: 14658522)
BMC Genomics. 2012 Apr 24;13:142. (PMID: 22524439)
Bioinformatics. 2005 Sep 15;21(18):3674-6. (PMID: 16081474)
Parasitology. 2004 Dec;129(Pt 6):693-702. (PMID: 15648692)
Trends Genet. 2000 Jun;16(6):276-7. (PMID: 10827456)
ISME J. 2015 Jul;9(7):1496-507. (PMID: 25500509)
J Immunol. 2014 Jul 15;193(2):773-82. (PMID: 24913976)
Eur J Biochem. 1999 Mar;260(2):414-20. (PMID: 10095776)
J Insect Physiol. 2007 Jul;53(7):715-23. (PMID: 17498733)
Parasitology. 2007 Jun;134(Pt 6):827-31. (PMID: 17306056)
PLoS One. 2012;7(5):e36264. (PMID: 22574143)
Parasitology. 2009 Dec;136(14):1943-9. (PMID: 19691861)
Nat Genet. 2011 May;43(5):491-8. (PMID: 21478889)
Mol Microbiol. 2006 Jun;60(5):1194-204. (PMID: 16689795)
Proc Natl Acad Sci U S A. 2001 Oct 23;98(22):12648-53. (PMID: 11592981)
PLoS Pathog. 2013;9(4):e1003318. (PMID: 23637607)
PLoS Pathog. 2010 Mar 05;6(3):e1000793. (PMID: 20221444)
Trends Parasitol. 2013 Mar;29(3):110-8. (PMID: 23375541)
Bull Soc Pathol Exot. 1994;87(5):391-3. (PMID: 7496207)
PLoS One. 2012;7(3):e33985. (PMID: 22479493)
Acta Trop. 2003 Oct;88(2):161-5. (PMID: 14516928)
Trans R Soc Trop Med Hyg. 1989 Sep-Oct;83(5):636-9. (PMID: 2617625)
Biol Chem. 1999 Dec;380(12):1455-9. (PMID: 10661875)
Mol Biol Evol. 2007 Jan;24(1):102-9. (PMID: 17012373)
Parasitology. 1989 Aug;99 Pt 1:57-66. (PMID: 2797872)
PLoS Negl Trop Dis. 2013 Aug 15;7(8):e2374. (PMID: 23967363)
BMC Microbiol. 2010 Jan 26;10:20. (PMID: 20102621)
Lancet. 2010 Jan 9;375(9709):148-59. (PMID: 19833383)
Acta Trop. 1986 Dec;43(4):407-8. (PMID: 2882668)
Bioinformatics. 2009 Jul 15;25(14):1754-60. (PMID: 19451168)
Nucleic Acids Res. 2010 Apr;38(6):1767-71. (PMID: 20015970)
BMC Genomics. 2013 Nov 13;14:783. (PMID: 24225185)
Insect Mol Biol. 2006 Aug;15(4):411-24. (PMID: 16907828)
Proc Natl Acad Sci U S A. 1997 Nov 11;94(23):12337-42. (PMID: 9356450)
Nat Genet. 2000 May;25(1):25-9. (PMID: 10802651)
Bioinformatics. 2003 Mar 22;19(5):651-2. (PMID: 12651724)
Parasitol Today. 1999 Oct;15(10):399-403. (PMID: 10481151)
J Biomed Biotechnol. 2010;2010:212817. (PMID: 20011064)
Genome Biol. 2003;4(10):R63. (PMID: 14519198)
فهرسة مساهمة: Keywords: Glossina palpalis gambiensis; RNA-seq; Trypanosoma brucei gambiense; de novo assembly; human African trypanosomiasis; in vivo metatranscriptomics
تواريخ الأحداث: Date Created: 20151201 Date Completed: 20151130 Latest Revision: 20200929
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC4643127
DOI: 10.3389/fmicb.2015.01259
PMID: 26617594
قاعدة البيانات: MEDLINE
الوصف
تدمد:1664-302X
DOI:10.3389/fmicb.2015.01259