دورية أكاديمية
Interferon-λ1 suppresses invasion and enhances autophagy in human osteosarcoma cell.
| العنوان: | Interferon-λ1 suppresses invasion and enhances autophagy in human osteosarcoma cell. |
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| المؤلفون: | Gao D; Department of Clinical Laboratory, Third Affiliated Hospital of Anhui Medical University Hefei 230032, China., Zhao J; Department of Microbiology, Anhui Medical University 69 Meishan Road, Hefei 230032, China., Li X; Department of Clinical Laboratory, Third Affiliated Hospital of Anhui Medical University Hefei 230032, China., Xia Y; Department of Clinical Laboratory, Third Affiliated Hospital of Anhui Medical University Hefei 230032, China., Cai Y; Department of Clinical Laboratory, Third Affiliated Hospital of Anhui Medical University Hefei 230032, China., Pan J; Department of Clinical Laboratory, Third Affiliated Hospital of Anhui Medical University Hefei 230032, China., Zhou H; Department of Clinical Laboratory, Third Affiliated Hospital of Anhui Medical University Hefei 230032, China., Fang Y; Department of Clinical Laboratory, Third Affiliated Hospital of Anhui Medical University Hefei 230032, China., Zhang S; Department of Clinical Laboratory, Third Affiliated Hospital of Anhui Medical University Hefei 230032, China., Wen H; Department of Clinical Laboratory, Third Affiliated Hospital of Anhui Medical University Hefei 230032, China. |
| المصدر: | International journal of clinical and experimental medicine [Int J Clin Exp Med] 2015 Sep 15; Vol. 8 (9), pp. 14999-5004. Date of Electronic Publication: 2015 Sep 15 (Print Publication: 2015). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: e-Century Pub. Corp Country of Publication: United States NLM ID: 101471010 Publication Model: eCollection Cited Medium: Print ISSN: 1940-5901 (Print) Linking ISSN: 19405901 NLM ISO Abbreviation: Int J Clin Exp Med Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: Madison, WI : e-Century Pub. Corp. |
| مستخلص: | Objective: The purpose of the present study was to determine whether type III IFN can modulate the autophagic response in human osteosarcoma cell. Methods: Human osteosarcoma cell were treated with Interferon-λ1. We investigated that Interferon-λ1 could inhibit the invasive ability of osteosarcoma cells by Matrigel invasion assay. Autophagy were assessed by acridine orange staining, MDC staining and Transmission electron microscopy. Results: In this study, we found that Interferon-λ1 could inhibit the invasive ability of osteosarcoma cells. Acridine orange staining and MDC staining showed that Interferon-λ1 triggered the accumulation of acidic vesicular and autolysosomes in osteosarcoma cell. The acridine orange osteosarcoma cell ratios were 3.6 ± 0.5%, 4.5 ± 0.8%, and 12.4 ± 1.7% after treatment with 1, 10, and 100 ng/mL Interferon-λ1 for 48 h. Osteosarcoma cell cells treated with 100 ng/mL Interferon-λ1 for 48 h developed autophagy some-like characteristics, including single or double-membrane vacuoles containing intact and degraded cellular debris. Conclusions: Interferon-λ1 could inhibit the invasive ability of osteosarcoma cells. Autophagy can be induced in a dose-dependent manner by treatment with Interferon-λ1 in osteosarcoma cell. |
| References: | Hepatology. 2014 Apr;59(4):1262-72. (PMID: 23929627) Semin Cancer Biol. 2015 Apr;31:99-105. (PMID: 24933034) J Innate Immun. 2013;5(3):209-18. (PMID: 23207147) Front Biosci (Landmark Ed). 2013 Jun 01;18:909-18. (PMID: 23747856) Cancer Res. 2014 Feb 1;74(3):647-51. (PMID: 24459182) PLoS One. 2013 May 16;8(5):e63940. (PMID: 23696859) Clin Dev Immunol. 2011;2011:479013. (PMID: 22013482) Mol Pharmacol. 2014 Jun;85(6):830-8. (PMID: 24574520) J Leukoc Biol. 2009 Jul;86(1):23-32. (PMID: 19304895) J Immunol. 2011 Mar 1;186(5):3138-47. (PMID: 21278349) Vet Immunol Immunopathol. 2013 Nov 15;156(1-2):141-6. (PMID: 24126210) Biochem Biophys Res Commun. 2006 Jun 16;344(4):1334-41. (PMID: 16650825) J Interferon Cytokine Res. 2010 Aug;30(8):555-64. (PMID: 20712453) |
| فهرسة مساهمة: | Keywords: Interferon-λ1; acridine orange; autophagy; transmission electron microscopy |
| تواريخ الأحداث: | Date Created: 20151203 Date Completed: 20151202 Latest Revision: 20200929 |
| رمز التحديث: | 20221213 |
| مُعرف محوري في PubMed: | PMC4658872 |
| PMID: | 26628983 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1940-5901 |
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