دورية أكاديمية
أعرض في EDS

Bleeding the laboratory mouse: Not all methods are equal.

التفاصيل البيبلوغرافية
العنوان: Bleeding the laboratory mouse: Not all methods are equal.
المؤلفون: Hoggatt J; Cancer Center/Center for Transplantation Sciences, Massachusetts General Hospital, Harvard Medical School, Boston, MA. Electronic address: hoggatt.jonathan@mgh.harvard.edu., Hoggatt AF; Center for Comparative Medicine, Harvard Medical School, Boston, MA., Tate TA; Cancer Center/Center for Transplantation Sciences, Massachusetts General Hospital, Harvard Medical School, Boston, MA., Fortman J; Biologic Resources Laboratory, University of Illinois at Chicago, Chicago, IL., Pelus LM; Microbiology and Immunology, Indiana University School of Medicine, Indianapolis, IN. Electronic address: lpelus@iupui.edu.
المصدر: Experimental hematology [Exp Hematol] 2016 Feb; Vol. 44 (2), pp. 132-137.e1. Date of Electronic Publication: 2015 Nov 28.
نوع المنشور: Journal Article; Research Support, N.I.H., Extramural
اللغة: English
بيانات الدورية: Publisher: Elsevier Science Inc Country of Publication: Netherlands NLM ID: 0402313 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2399 (Electronic) Linking ISSN: 0301472X NLM ISO Abbreviation: Exp Hematol Subsets: MEDLINE
أسماء مطبوعة: Publication: 1999- : Amsterdam : Elsevier Science Inc.
Original Publication: Copenhagen, Munksgaard.
مواضيع طبية MeSH: Animals, Laboratory* , Blood* , Specimen Handling*, Animals ; Mice
مستخلص: The laboratory mouse is the model most frequently used in hematologic studies and assessment of blood parameters across a broad range of disciplines. Often, analysis of blood occurs in a nonterminal manner. However, the small body size of the mouse limits collection based on volume, frequency, and accessible sites. Commonly used sites in the mouse include the retro-orbital sinus, facial vein, tail vein, saphenous vein, and heart. The method of blood acquisition varies considerably across laboratories and is often not reported in detail. In this study, we report significant alterations in blood parameters, particularly of total white blood cells, specific populations of dendritic cells and myeloid-derived suppressor cells, and hematopoietic progenitor cells, as a result of site and manner of sampling. Intriguingly, warming of mice prior to tail bleeding was found to significantly alter blood values. Our findings suggest that the same method should be used across an entire study, that mice should be warmed prior to tail bleeds to make levels uniform, and that accurate description of bleeding methods in publications should be provided to allow for interpretation of comparative reports and inter- and intralaboratory experimental variability.
(Copyright © 2016 ISEH - International Society for Experimental Hematology. Published by Elsevier Inc. All rights reserved.)
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معلومات مُعتمدة: T32 HL007910 United States HL NHLBI NIH HHS; R01 HL096305 United States HL NHLBI NIH HHS; HL096305 United States HL NHLBI NIH HHS; P30 CA082709 United States CA NCI NIH HHS; HL069669 United States HL NHLBI NIH HHS; P30CA082709 United States CA NCI NIH HHS; K99 HL119559 United States HL NHLBI NIH HHS; HL119559 United States HL NHLBI NIH HHS; HL007910 United States HL NHLBI NIH HHS; R01 HL069669 United States HL NHLBI NIH HHS; R00 HL119559 United States HL NHLBI NIH HHS
تواريخ الأحداث: Date Created: 20151209 Date Completed: 20160527 Latest Revision: 20181113
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC5810935
DOI: 10.1016/j.exphem.2015.10.008
PMID: 26644183
قاعدة البيانات: MEDLINE
الوصف
تدمد:1873-2399
DOI:10.1016/j.exphem.2015.10.008