دورية أكاديمية
Antimicrobial evaluation of quaternary ammonium polyethyleneimine nanoparticles against clinical isolates of pathogenic bacteria.
| العنوان: | Antimicrobial evaluation of quaternary ammonium polyethyleneimine nanoparticles against clinical isolates of pathogenic bacteria. |
|---|---|
| المؤلفون: | Ortega A; BIOTYC Foundation, C/ Blasco de Garay, 27., 02003 Albacete, Spain., Farah S; Institute of Drug Research, School of Pharmacy-Faculty of Medicine, Center for Nanoscience and Nanotechnology and The Alex Grass Center for Drug Design and Synthesis, The Hebrew University of Jerusalem, Jerusalem 91120, Israel., Tranque P; Instituto de Investigación en Discapacidades Neurológicas (IDINE), Universidad de Castilla-La Mancha, C/ Almansa, 14., 02006 Albacete, Spain., Ocaña AV; Department of Nephrology, Complejo Hospitalario Universitario, C/ Hermanos Falco., 02006 Albacete, Spain., Nam-Cha SH; Department of Pathology, Complejo Hospitalario Universitario, C/ Hermanos Falco., 02006 Albacete, Spain., Beyth N; Prosthodontics Unit, Faculty of Dentistry, The Hebrew University Jerusalem, Jerusalem 91120, Israel., Gómez-Roldán C; Department of Nephrology, Complejo Hospitalario Universitario, C/ Hermanos Falco., 02006 Albacete, Spain., Pérez-Tanoira R; Division of Infectious Diseases and Clinical Microbiology, Fundación Jiménez Díaz, Universidad Autónoma de Madrid, Ave. Reyes Católicos, 2, 28040 Madrid, Spain., Domb AJ; Institute of Drug Research, School of Pharmacy-Faculty of Medicine, Center for Nanoscience and Nanotechnology and The Alex Grass Center for Drug Design and Synthesis, The Hebrew University of Jerusalem, Jerusalem 91120, Israel., Pérez-Martínez FC; Department of Research and Development, Nanocustom S.L., 02007 Albacete, Spain. fperez@nanocustom.es., Pérez-Martínez J; BIOTYC Foundation, C/ Blasco de Garay, 27., 02003 Albacete, Spain. |
| المصدر: | IET nanobiotechnology [IET Nanobiotechnol] 2015 Dec; Vol. 9 (6), pp. 342-8. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Hindawi Pub. Corp Country of Publication: United States NLM ID: 101303205 Publication Model: Print Cited Medium: Print ISSN: 1751-8741 (Print) Linking ISSN: 17518741 NLM ISO Abbreviation: IET Nanobiotechnol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2023- : [London] : Hindawi Pub. Corp. Original Publication: Stevenage, Herts, UK : Institution of Engineering and Technology, c2007- |
| مواضيع طبية MeSH: | Bacterial Physiological Phenomena/*drug effects , Nanoparticles/*administration & dosage , Nanoparticles/*chemistry , Peritonitis/*microbiology , Polyethyleneimine/*administration & dosage , Quaternary Ammonium Compounds/*administration & dosage, Animals ; Anti-Bacterial Agents/administration & dosage ; Anti-Bacterial Agents/chemical synthesis ; Cell Survival/drug effects ; Dose-Response Relationship, Drug ; Humans ; Materials Testing ; Nanoparticles/ultrastructure ; Particle Size ; Peritonitis/drug therapy ; Polyethyleneimine/chemistry ; Quaternary Ammonium Compounds/chemistry |
| مستخلص: | Peritonitis is a disease caused by bacterial strains that have become increasingly resistant to many antibiotics. The development of alternative therapeutic compounds is the focus of extensive research, so novel nanoparticles (NPs) with activity against antibiotic-resistant bacteria should be developed. In this study, the antibacterial activity of quaternary ammonium polyethyleneimine (QA-PEI) NPs was evaluated against Streptococcus viridans, Stenotrophomonas maltophilia and Escherichia coli. To appraise the antibacterial activity, minimal inhibitory concentration (MIC), minimal bactericidal concentration and bactericidal assays were utilised with different concentrations (1.56-100 µg/ml) of QA-PEI NPs. Moreover, 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) and annexin V/propidium iodide toxicity assays were performed in cell cultures. MICs for S. maltophilia and E. coli isolates were 12.5 and 25 µg/ml, respectively, whereas the MIC for S. viridans was 100 µg/ml. Furthermore, the growth curve assays revealed that these QA-PEI NPs at a concentration of 12.5 µg/ml significantly inhibited bacterial growth for the bacterial isolates studied. On the other hand, QA-PEI NPs lacked significant toxicity for cells when used at concentrations up to 50 μg/ml for 48 h. The present findings reveal the potential therapeutic value of this QA-PEI NPs as alternative antibacterial agents for peritonitis, especially against Gram-negative bacteria. |
| المشرفين على المادة: | 0 (Anti-Bacterial Agents) 0 (Quaternary Ammonium Compounds) 9002-98-6 (Polyethyleneimine) |
| تواريخ الأحداث: | Date Created: 20151210 Date Completed: 20160408 Latest Revision: 20161021 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1049/iet-nbt.2014.0078 |
| PMID: | 26647809 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1751-8741 |
|---|---|
| DOI: | 10.1049/iet-nbt.2014.0078 |