دورية أكاديمية
A Gene Encoding a DUF247 Domain Protein Cosegregates with the S Self-Incompatibility Locus in Perennial Ryegrass.
| العنوان: | A Gene Encoding a DUF247 Domain Protein Cosegregates with the S Self-Incompatibility Locus in Perennial Ryegrass. |
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| المؤلفون: | Manzanares C; Forage Crop Genetics, Institute of Agricultural Sciences, ETH Zurich, Zurich, Switzerland Teagasc Crops, Environment and Land Use Programme, Oak Park Research Centre, Carlow, Ireland Institute of Biological, Environmental and Rural Sciences, Aberystwyth University, Plas Gogerddan, Aberystwyth, Ceredigion, United Kingdom., Barth S; Teagasc Crops, Environment and Land Use Programme, Oak Park Research Centre, Carlow, Ireland., Thorogood D; Institute of Biological, Environmental and Rural Sciences, Aberystwyth University, Plas Gogerddan, Aberystwyth, Ceredigion, United Kingdom., Byrne SL; Department of Molecular Biology and Genetics, Research Centre Flakkebjerg, Aarhus University, Slagelse, Denmark., Yates S; Forage Crop Genetics, Institute of Agricultural Sciences, ETH Zurich, Zurich, Switzerland., Czaban A; Department of Molecular Biology and Genetics, Research Centre Flakkebjerg, Aarhus University, Slagelse, Denmark., Asp T; Department of Molecular Biology and Genetics, Research Centre Flakkebjerg, Aarhus University, Slagelse, Denmark., Yang B; BGI-Shenzhen, Building 1, Beishan Industrial Zone, Yantian District, Shenzhen, China., Studer B; Forage Crop Genetics, Institute of Agricultural Sciences, ETH Zurich, Zurich, Switzerland bruno.studer@usys.ethz.ch. |
| المصدر: | Molecular biology and evolution [Mol Biol Evol] 2016 Apr; Vol. 33 (4), pp. 870-84. Date of Electronic Publication: 2015 Dec 10. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Oxford University Press Country of Publication: United States NLM ID: 8501455 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1537-1719 (Electronic) Linking ISSN: 07374038 NLM ISO Abbreviation: Mol Biol Evol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2003- : New York, NY : Oxford University Press Original Publication: [Chicago, Ill.] : University of Chicago Press, [c1983- |
| مواضيع طبية MeSH: | Chromosome Mapping*, Plant Proteins/*genetics , Plant Weeds/*genetics , Self-Incompatibility in Flowering Plants/*genetics, Genetic Linkage ; Genotype ; High-Throughput Nucleotide Sequencing ; Protein Kinases/genetics |
| مستخلص: | The grass family (Poaceae), the fourth largest family of flowering plants, encompasses the most economically important cereal, forage, and energy crops, and exhibits a unique gametophytic self-incompatibility (SI) mechanism that is controlled by at least two multiallelic and independent loci, S and Z. Despite intense research efforts over the last six decades, the genes underlying S and Z remain uncharacterized. Here, we report a fine-mapping approach to identify the male component of the S-locus in perennial ryegrass (Lolium perenne L.) and provide multiple evidence that a domain of unknown function 247 (DUF247) gene is involved in its determination. Using a total of 10,177 individuals from seven different mapping populations segregating for S, we narrowed the S-locus to a genomic region containing eight genes, the closest recombinant marker mapping at a distance of 0.016 cM. Of the eight genes cosegregating with the S-locus, a highly polymorphic gene encoding for a protein containing a DUF247 was fully predictive of known S-locus genotypes at the amino acid level in the seven mapping populations. Strikingly, this gene showed a frameshift mutation in self-compatible darnel (Lolium temulentum L.), whereas all of the self-incompatible species of the Festuca-Lolium complex were predicted to encode functional proteins. Our results represent a major step forward toward understanding the gametophytic SI system in one of the most important plant families and will enable the identification of additional components interacting with the S-locus. (© The Author(s) 2015. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.) |
| معلومات مُعتمدة: | BB/J004405/1 United Kingdom Biotechnology and Biological Sciences Research Council |
| فهرسة مساهمة: | Keywords: RNA sequencing (RNAseq); S-locus; domain of unknown function (DUF) 247; fine-mapping; perennial ryegrass (Lolium perenne L.); self-incompatibility (SI) |
| المشرفين على المادة: | 0 (Plant Proteins) EC 2.7.- (Protein Kinases) EC 2.7.11.1 (S-receptor kinase) |
| تواريخ الأحداث: | Date Created: 20151215 Date Completed: 20161213 Latest Revision: 20220316 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1093/molbev/msv335 |
| PMID: | 26659250 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1537-1719 |
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| DOI: | 10.1093/molbev/msv335 |