دورية أكاديمية
Involvement of CC Chemokine Receptor 1 and CCL3 in Acute and Chronic Inflammatory Pain in Mice.
| العنوان: | Involvement of CC Chemokine Receptor 1 and CCL3 in Acute and Chronic Inflammatory Pain in Mice. |
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| المؤلفون: | Llorián-Salvador M; Laboratory of Pharmacology, School of Medicine, University Institute of Oncology of Asturias (IUOPA), University of Oviedo, Asturias, Spain., González-Rodríguez S; Laboratory of Pharmacology, School of Medicine, University Institute of Oncology of Asturias (IUOPA), University of Oviedo, Asturias, Spain., Lastra A; Laboratory of Pharmacology, School of Medicine, University Institute of Oncology of Asturias (IUOPA), University of Oviedo, Asturias, Spain., Fernández-García MT; Molecular Histopathology in Animal Models of Cancer Unit, IUOPA, University of Oviedo, Asturias, Spain., Hidalgo A; Laboratory of Pharmacology, School of Medicine, University Institute of Oncology of Asturias (IUOPA), University of Oviedo, Asturias, Spain., Menéndez L; Laboratory of Pharmacology, School of Medicine, University Institute of Oncology of Asturias (IUOPA), University of Oviedo, Asturias, Spain., Baamonde A; Laboratory of Pharmacology, School of Medicine, University Institute of Oncology of Asturias (IUOPA), University of Oviedo, Asturias, Spain. |
| المصدر: | Basic & clinical pharmacology & toxicology [Basic Clin Pharmacol Toxicol] 2016 Jul; Vol. 119 (1), pp. 32-40. Date of Electronic Publication: 2016 Jan 14. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Blackwell Country of Publication: England NLM ID: 101208422 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1742-7843 (Electronic) Linking ISSN: 17427835 NLM ISO Abbreviation: Basic Clin Pharmacol Toxicol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2005-> : Oxford : Blackwell Original Publication: Copenhagen, Denmark : Oxford, UK : Nordic Pharmacological Society Distributed by Blackwell Munksgaard, 2004- |
| مواضيع طبية MeSH: | Acute Pain/*metabolism , Chemokine CCL3/*metabolism , Chronic Pain/*metabolism , Inflammation/*metabolism , Receptors, CCR1/*metabolism, Acute Pain/chemically induced ; Acute Pain/drug therapy ; Analgesics/pharmacology ; Animals ; Anti-Inflammatory Agents/pharmacology ; Carrageenan/toxicity ; Chemokine CCL3/genetics ; Chemokine CCL5/genetics ; Chemokine CCL5/metabolism ; Chronic Pain/chemically induced ; Chronic Pain/drug therapy ; Disease Models, Animal ; Dose-Response Relationship, Drug ; Freund's Adjuvant/toxicity ; Hyperalgesia/chemically induced ; Hyperalgesia/drug therapy ; Inflammation/chemically induced ; Inflammation/drug therapy ; Male ; Mice ; Neutrophils/drug effects ; Neutrophils/metabolism ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; Receptors, CCR1/antagonists & inhibitors ; Receptors, CCR1/genetics ; Spinal Cord/drug effects ; Spinal Cord/metabolism ; Xanthenes/pharmacology |
| مستخلص: | Chemokines are chemotactic cytokines whose involvement in nociceptive processing is being increasingly recognized. Based on the previous description of the involvement of CC chemokine receptor type 1 (CCR1) in pathological pain, we have assessed the participation of CCR1 and its endogenous ligands CCL3 and CCL5 in hyperalgesia and allodynia in mice after acute inflammation with carrageenan and chronic inflammation with complete Freund's adjuvant (CFA). The subcutaneous administration of the CCR1 antagonist J113863 (3-30 mg/kg; 30 min. before) dose dependently inhibited carrageenan- and CFA-evoked thermal hyperalgesia and mechanical allodynia produced by CFA, but not by carrageenan. The maximal dose of J113863 did not modify the increase in paw thickness induced by carrageenan or CFA. An almost ten times augmentation of CCL3 levels was detected by ELISA assays in both carrageenan and CFA paws, but not in spinal cords of inflamed mice, whereas CCL5 concentrations remained unaltered. Accordingly, a marked increase of CCL3 mRNA expression was observed in inflamed paws, with CCL3 protein detected in neutrophils and macrophages by immunohistochemical experiments. The intraplantar administration of an anti-CCL3 antibody (0.3-3 μg) blocked thermal hyperalgesia in carrageenan- and CFA-inflamed mice as well as CFA-evoked mechanical allodynia. Our data suggest that the increased concentrations of CCL3 present in inflamed tissues can be involved in acute and chronic inflammatory hyperalgesia as well as in chronic mechanical allodynia, and that these hypernociceptive symptoms can be counteracted by its neutralization with an antibody or by the blockade of CCR1 receptors. (© 2015 Nordic Association for the Publication of BCPT (former Nordic Pharmacological Society).) |
| سلسلة جزيئية: | GENBANK NM_007393.4; NM_011337 |
| المشرفين على المادة: | 0 (Analgesics) 0 (Anti-Inflammatory Agents) 0 (Ccl3 protein, mouse) 0 (Ccl5 protein, mouse) 0 (Ccr1 protein, mouse) 0 (Chemokine CCL3) 0 (Chemokine CCL5) 0 (RNA, Messenger) 0 (Receptors, CCR1) 0 (Xanthenes) 9000-07-1 (Carrageenan) 9007-81-2 (Freund's Adjuvant) E32U2ESR7J (UCB 35625) |
| تواريخ الأحداث: | Date Created: 20151215 Date Completed: 20170308 Latest Revision: 20220408 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1111/bcpt.12543 |
| PMID: | 26663750 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1742-7843 |
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| DOI: | 10.1111/bcpt.12543 |