دورية أكاديمية
أعرض في EDS

Live Cell Discovery of Microbial Vitamin Transport and Enzyme-Cofactor Interactions.

التفاصيل البيبلوغرافية
العنوان: Live Cell Discovery of Microbial Vitamin Transport and Enzyme-Cofactor Interactions.
المؤلفون: Anderson LN; Biological Sciences Division, Pacific Northwest National Laboratory , Richland, Washington 99352 United States., Koech PK; Biological Sciences Division, Pacific Northwest National Laboratory , Richland, Washington 99352 United States., Plymale AE; Biological Sciences Division, Pacific Northwest National Laboratory , Richland, Washington 99352 United States., Landorf EV; Biosciences Division, Argonne National Laboratory , Argonne, Illinois 60439 United States., Konopka A; Biological Sciences Division, Pacific Northwest National Laboratory , Richland, Washington 99352 United States., Collart FR; Biosciences Division, Argonne National Laboratory , Argonne, Illinois 60439 United States., Lipton MS; Biological Sciences Division, Pacific Northwest National Laboratory , Richland, Washington 99352 United States., Romine MF; Biological Sciences Division, Pacific Northwest National Laboratory , Richland, Washington 99352 United States., Wright AT; Biological Sciences Division, Pacific Northwest National Laboratory , Richland, Washington 99352 United States.
المصدر: ACS chemical biology [ACS Chem Biol] 2016 Feb 19; Vol. 11 (2), pp. 345-54. Date of Electronic Publication: 2015 Dec 22.
نوع المنشور: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.
اللغة: English
بيانات الدورية: Publisher: American Chemical Society Country of Publication: United States NLM ID: 101282906 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1554-8937 (Electronic) Linking ISSN: 15548929 NLM ISO Abbreviation: ACS Chem Biol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Washington, D.C. : American Chemical Society, c2006-
مواضيع طبية MeSH: Bacterial Proteins/*metabolism , Chloroflexus/*metabolism , Vitamin B Complex/*metabolism, Biological Transport ; Chloroflexus/cytology ; Molecular Probe Techniques ; Optical Imaging ; Proteome/metabolism ; Staining and Labeling
مستخلص: The rapid completion of microbial genomes is inducing a conundrum in functional gene discovery. Novel methods are needed to shorten the gap between characterizing a microbial genome and experimentally validating bioinformatically predicted functions. Of particular importance are transport mechanisms, which shuttle nutrients such as B vitamins and metabolites across cell membranes and are required for the survival of microbes ranging from members of environmental microbial communities to pathogens. Methods to accurately assign function and specificity for a wide range of experimentally unidentified and/or predicted membrane-embedded transport proteins, along with characterization of intracellular enzyme-cofactor associations, are needed to enable a significantly improved understanding of microbial biochemistry and physiology, microbial interactions, and microbial responses to perturbations. Chemical probes derived from B vitamins B1, B2, and B7 have allowed us to experimentally address the aforementioned needs by identifying B vitamin transporters and intracellular enzyme-cofactor associations through live cell labeling of the filamentous anoxygenic photoheterotroph, Chloroflexus aurantiacus J-10-fl, known to employ mechanisms for both B vitamin biosynthesis and environmental salvage. Our probes provide a unique opportunity to directly link cellular activity and protein function back to ecosystem and/or host dynamics by identifying B vitamin transport and cofactor-dependent interactions required for survival.
المشرفين على المادة: 0 (Bacterial Proteins)
0 (Proteome)
12001-76-2 (Vitamin B Complex)
تواريخ الأحداث: Date Created: 20151217 Date Completed: 20161026 Latest Revision: 20220310
رمز التحديث: 20240628
DOI: 10.1021/acschembio.5b00918
PMID: 26669591
قاعدة البيانات: MEDLINE
الوصف
تدمد:1554-8937
DOI:10.1021/acschembio.5b00918