دورية أكاديمية
17α-Ethinylestradiol (EE2) treatment of wild roach (Rutilus rutilus) during early life development disrupts expression of genes directly involved in the feedback cycle of estrogen.
| العنوان: | 17α-Ethinylestradiol (EE2) treatment of wild roach (Rutilus rutilus) during early life development disrupts expression of genes directly involved in the feedback cycle of estrogen. |
|---|---|
| المؤلفون: | Nikoleris L; Centre for Environmental and Climate Research (CEC), Lund University, SE-223 62 Lund, Sweden; Section for Biodiversity, Department of Biology, Lund University, Ecology Building, SE-223 62 Lund, Sweden., Hultin CL; Centre for Environmental and Climate Research (CEC), Lund University, SE-223 62 Lund, Sweden., Hallgren P; Section for Aquatic Ecology, Department of Biology, Lund University, Ecology Building, SE-223 62 Lund, Sweden., Hansson MC; Centre for Environmental and Climate Research (CEC), Lund University, SE-223 62 Lund, Sweden. Electronic address: Maria.Hansson@cec.lu.se. |
| المصدر: | Comparative biochemistry and physiology. Toxicology & pharmacology : CBP [Comp Biochem Physiol C Toxicol Pharmacol] 2016 Feb; Vol. 180, pp. 56-64. Date of Electronic Publication: 2015 Dec 10. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Science Country of Publication: United States NLM ID: 100959500 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1532-0456 (Print) Linking ISSN: 15320456 NLM ISO Abbreviation: Comp Biochem Physiol C Toxicol Pharmacol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: New York, NY : Elsevier Science, c2000- |
| مواضيع طبية MeSH: | Cyprinidae/*growth & development , Estrogens/*metabolism , Ethinyl Estradiol/*toxicity , Gene Expression Regulation, Developmental/*drug effects, Animals ; Female ; Male ; Transcriptome ; Water Pollutants, Chemical/toxicity |
| مستخلص: | Fish are more sensitive to introduced disturbances from synthetic endocrine disrupting compounds during early life phases compared with mature stages. 17α-Ethinylestradiol (EE2), which is the active compound in human oral contraceptives and hormone replacement therapies, is today ever present in the effluents from sewage treatment plants. EE2 targets and interacts with the endogenous biological systems of exposed vertebrates resulting in to large extents unknown short- and long-term effects. We investigated how EE2 exposure affects expression profiles of a large number of target genes during early life of roach (Rutilus rutilus). We exposed fertilized roach eggs collected from a lake in Southern Sweden to EE2 for 12weeks together with 1+-year-old roach in aquaria. We measured the gene expression of the estrogen receptor (esr)1/2a/2b, androgen receptor (ar), vitellogenin, cytochrome P450 (cyp)19a1a/1b in fertilized eggs; newly hatched larvae; 12-week-old fry; and juvenile wild roach (1+-year-old). Results shows that an EE2 concentration as low as 0.5ng/L significantly affects gene expression during early development. Gene expression responses vary both among life stages and molecular receptors. We also show that the gene profile of the estrogen feedback cycle to a large extent depends on the relationship between the three esr genes and the two cyp19a1 genes, which are all up-regulated with age. Results indicate that a disruption of the natural activity of the dominant esr gene could lead to detrimental biological effects if EE2 exposure occurs during development, even if this exposure occurred for only a short period. (Copyright © 2015. Published by Elsevier Inc.) |
| فهرسة مساهمة: | Keywords: 17α-Ethinyl estradiol; Biomarkers; Cytochrome P450; Embryonic development; Endocrine disruptors; Estrogen receptor; Gene regulation; Roach |
| المشرفين على المادة: | 0 (Estrogens) 0 (Water Pollutants, Chemical) 423D2T571U (Ethinyl Estradiol) |
| تواريخ الأحداث: | Date Created: 20151223 Date Completed: 20161006 Latest Revision: 20161230 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1016/j.cbpc.2015.12.002 |
| PMID: | 26689641 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1532-0456 |
|---|---|
| DOI: | 10.1016/j.cbpc.2015.12.002 |