دورية أكاديمية
أعرض في EDS

Production of Brazilian human norovirus VLPs and comparison of purification methods.

التفاصيل البيبلوغرافية
العنوان: Production of Brazilian human norovirus VLPs and comparison of purification methods.
المؤلفون: Lamounier TA; Departamento de Farmácia, Universidade de Brasília, Brasília, DF, Brazil., de Oliveira LM; Departamento de Biologia Celular, Universidade de Brasília, Brasília, DF, Brazil., de Camargo BR; Departamento de Biologia Celular, Universidade de Brasília, Brasília, DF, Brazil., Rodrigues KB; Departamento de Biologia Celular, Universidade de Brasília, Brasília, DF, Brazil., Noronha EF; Departamento de Biologia Celular, Universidade de Brasília, Brasília, DF, Brazil., Ribeiro BM; Departamento de Biologia Celular, Universidade de Brasília, Brasília, DF, Brazil., Nagata T; Departamento de Biologia Celular, Universidade de Brasília, Brasília, DF, Brazil.
المصدر: Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] [Braz J Microbiol] 2015 Oct-Dec; Vol. 46 (4), pp. 1265-8. Date of Electronic Publication: 2015 Oct 09.
نوع المنشور: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Springer International Publishing Country of Publication: Brazil NLM ID: 101095924 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1678-4405 (Electronic) Linking ISSN: 15178382 NLM ISO Abbreviation: Braz J Microbiol Subsets: MEDLINE
أسماء مطبوعة: Publication: 2019- : Switzerland, AG : Springer International Publishing
Original Publication: Rio de Janeiro, RJ, Brasil : Sociedade Brasileira de Microbiologia
مواضيع طبية MeSH: Centrifugation, Density Gradient/*methods , Chromatography, Ion Exchange/*methods , Norovirus/*genetics , Viral Structural Proteins/*genetics , Virosomes/*isolation & purification, Brazil ; Child ; Humans ; Viral Structural Proteins/metabolism ; Virosomes/genetics ; Virosomes/metabolism
مستخلص: Noroviruses (NVs) are responsible for most cases of human nonbacterial gastroenteritis worldwide. Some parameters for the purification of NV virus-like particles (VLPs) such as ease of production and yield were studied for future development of vaccines and diagnostic tools. In this study, VLPs were produced by the expression of the VP1 and VP2 gene cassette of the Brazilian NV isolate, and two purification methods were compared: cesium chloride (CsCl) gradient centrifugation and ion-exchange chromatography (IEC). IEC produced more and purer VLPs of NV compared to CsCl gradient centrifugation.
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المشرفين على المادة: 0 (Viral Structural Proteins)
0 (Virosomes)
تواريخ الأحداث: Date Created: 20151223 Date Completed: 20161006 Latest Revision: 20181113
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC4704647
DOI: 10.1590/S1517-838246420140925
PMID: 26691489
قاعدة البيانات: MEDLINE
الوصف
تدمد:1678-4405
DOI:10.1590/S1517-838246420140925