دورية أكاديمية
أعرض في EDS

Aminoguanidine treatment increased NOX2 response in diabetic rats: Improved phagocytosis and killing of Candida albicans by neutrophils.

التفاصيل البيبلوغرافية
العنوان: Aminoguanidine treatment increased NOX2 response in diabetic rats: Improved phagocytosis and killing of Candida albicans by neutrophils.
المؤلفون: de Souza Ferreira C; Departamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas, UNIFAL-MG, Alfenas, MG, Brazil., Pennacchi PC; Departamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas, UNIFAL-MG, Alfenas, MG, Brazil., Araújo TH; Departamento de Histologia, Instituto de Ciências Biomédicas, UNIFAL MG, Alfenas, MG, Brazil., Taniwaki NN; Setor de Virologia, Instituto Adolfo Lutz, São Paulo, SP, Brazil., de Araújo Paula FB; Departamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas, UNIFAL-MG, Alfenas, MG, Brazil., da Silveira Duarte SM; Departamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas, UNIFAL-MG, Alfenas, MG, Brazil., Rodrigues MR; Departamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas, UNIFAL-MG, Alfenas, MG, Brazil. Electronic address: mritar11@yahoo.com.br.
المصدر: European journal of pharmacology [Eur J Pharmacol] 2016 Feb 05; Vol. 772, pp. 83-91. Date of Electronic Publication: 2015 Dec 24.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Elsevier Science Country of Publication: Netherlands NLM ID: 1254354 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0712 (Electronic) Linking ISSN: 00142999 NLM ISO Abbreviation: Eur J Pharmacol Subsets: MEDLINE
أسماء مطبوعة: Publication: 2005- : Amsterdam : Elsevier Science
Original Publication: Amsterdam, North Holland Pub. Co.
مواضيع طبية MeSH: Candida albicans*, Diabetes Mellitus, Experimental/*enzymology , Guanidines/*pharmacology , Membrane Glycoproteins/*metabolism , NADPH Oxidases/*metabolism , Neutrophils/*drug effects , Neutrophils/*immunology , Phagocytosis/*drug effects, Animals ; Diabetes Mellitus, Experimental/immunology ; Diabetes Mellitus, Experimental/microbiology ; Glycated Hemoglobin/metabolism ; Glycation End Products, Advanced/metabolism ; Immunologic Factors/pharmacology ; Kidney/drug effects ; Kidney/pathology ; Male ; NADPH Oxidase 2 ; Neutrophils/metabolism ; Rats ; Rats, Wistar ; Reactive Oxygen Species/metabolism
مستخلص: In this study, we show that aminoguanidine (AMG), an inhibitor of protein glycation, increases the NOX2 (phagocyte NADPH oxidase) response and microbicidal activity by neutrophils, regardless of diabetic status. The non-enzymatic glycation of proteins, yielding irreversible advanced glycation end products (AGEs), is involved in the development of diabetes complications, including alterations of signaling pathways and the generation of reactive oxygen species by phagocytes. The phagocytes produce ROS (reactive oxygen species) through activation of the NOX2 complex, which generates superoxide. The purpose of this study was to evaluate the effect of hyperglycemia and the glycation of proteins on the NOX2 activity of neutrophils and its implications for cellular physiology, with a focus on the microbicidal activity of these cells. We treated diabetic rats with AMG and evaluated neutrophil ROS generation and Candida albicans killing ability. We observed a large increase in the microbicidal activity of peritoneal neutrophils from AMG-treated rats. The increase was independent of diabetic status and myeloperoxidase activity. Collectively, our results suggest that AMG has an immunomodulator role that triggers an increase in the microbicidal response of neutrophils mainly related to reactive oxygen species production by NOX2.
(Copyright © 2015 Elsevier B.V. All rights reserved.)
فهرسة مساهمة: Keywords: Aminoguanidine; Aminoguanidine bicarbonate (PubChem CID 164944); Candida albicans; Diabetes; Glycation of proteins; Neutrophils; Reactive Oxygen Species
المشرفين على المادة: 0 (Glycated Hemoglobin A)
0 (Glycation End Products, Advanced)
0 (Guanidines)
0 (Immunologic Factors)
0 (Membrane Glycoproteins)
0 (Reactive Oxygen Species)
EC 1.6.3.- (Cybb protein, rat)
EC 1.6.3.- (NADPH Oxidase 2)
EC 1.6.3.- (NADPH Oxidases)
SCQ4EZQ113 (pimagedine)
تواريخ الأحداث: Date Created: 20160103 Date Completed: 20161101 Latest Revision: 20221207
رمز التحديث: 20221213
DOI: 10.1016/j.ejphar.2015.12.044
PMID: 26724393
قاعدة البيانات: MEDLINE
الوصف
تدمد:1879-0712
DOI:10.1016/j.ejphar.2015.12.044