دورية أكاديمية
Reprogramming triggers endogenous L1 and Alu retrotransposition in human induced pluripotent stem cells.
العنوان: | Reprogramming triggers endogenous L1 and Alu retrotransposition in human induced pluripotent stem cells. |
---|---|
المؤلفون: | Klawitter S; Division of Medical Biotechnology, Paul-Ehrlich-Institute, D-63225 Langen, Germany., Fuchs NV; Division of Medical Biotechnology, Paul-Ehrlich-Institute, D-63225 Langen, Germany.; Max-Delbrück-Center for Molecular Medicine, D-13125 Berlin, Germany., Upton KR; Mater Research Institute, University of Queensland, TRI Building, Woolloongabba, Brisbane, Queensland 4102, Australia., Muñoz-Lopez M; Department of Human DNA Variability, Pfizer/University of Granada and Andalusian Regional Government Center for Genomics and Oncology (GENYO), PTS Granada, 18016 Granada, Spain., Shukla R; Mater Research Institute, University of Queensland, TRI Building, Woolloongabba, Brisbane, Queensland 4102, Australia., Wang J; Max-Delbrück-Center for Molecular Medicine, D-13125 Berlin, Germany., Garcia-Cañadas M; Department of Human DNA Variability, Pfizer/University of Granada and Andalusian Regional Government Center for Genomics and Oncology (GENYO), PTS Granada, 18016 Granada, Spain., Lopez-Ruiz C; Department of Human DNA Variability, Pfizer/University of Granada and Andalusian Regional Government Center for Genomics and Oncology (GENYO), PTS Granada, 18016 Granada, Spain., Gerhardt DJ; Mater Research Institute, University of Queensland, TRI Building, Woolloongabba, Brisbane, Queensland 4102, Australia., Sebe A; Division of Medical Biotechnology, Paul-Ehrlich-Institute, D-63225 Langen, Germany., Grabundzija I; Max-Delbrück-Center for Molecular Medicine, D-13125 Berlin, Germany., Merkert S; Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Department of Cardiac, Thoracic, Transplantation, and Vascular Surgery; REBIRTH, Cluster of Excellence, Hannover Medical School, D-30625 Hannover, Germany., Gerdes P; Mater Research Institute, University of Queensland, TRI Building, Woolloongabba, Brisbane, Queensland 4102, Australia., Pulgarin JA; Department of Human DNA Variability, Pfizer/University of Granada and Andalusian Regional Government Center for Genomics and Oncology (GENYO), PTS Granada, 18016 Granada, Spain., Bock A; Division of Medical Biotechnology, Paul-Ehrlich-Institute, D-63225 Langen, Germany., Held U; Division of Medical Biotechnology, Paul-Ehrlich-Institute, D-63225 Langen, Germany., Witthuhn A; Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Department of Cardiac, Thoracic, Transplantation, and Vascular Surgery; REBIRTH, Cluster of Excellence, Hannover Medical School, D-30625 Hannover, Germany., Haase A; Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Department of Cardiac, Thoracic, Transplantation, and Vascular Surgery; REBIRTH, Cluster of Excellence, Hannover Medical School, D-30625 Hannover, Germany., Sarkadi B; Department of Biophysics and Radiation Biology, Semmelweis University, H-1094 Budapest, Hungary., Löwer J; Division of Medical Biotechnology, Paul-Ehrlich-Institute, D-63225 Langen, Germany., Wolvetang EJ; Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, St Lucia, Queensland 4072, Australia., Martin U; Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Department of Cardiac, Thoracic, Transplantation, and Vascular Surgery; REBIRTH, Cluster of Excellence, Hannover Medical School, D-30625 Hannover, Germany., Ivics Z; Division of Medical Biotechnology, Paul-Ehrlich-Institute, D-63225 Langen, Germany., Izsvák Z; Max-Delbrück-Center for Molecular Medicine, D-13125 Berlin, Germany., Garcia-Perez JL; Department of Human DNA Variability, Pfizer/University of Granada and Andalusian Regional Government Center for Genomics and Oncology (GENYO), PTS Granada, 18016 Granada, Spain., Faulkner GJ; Mater Research Institute, University of Queensland, TRI Building, Woolloongabba, Brisbane, Queensland 4102, Australia.; Queensland Brain Institute, University of Queensland, Brisbane, Queensland 4072, Australia., Schumann GG; Division of Medical Biotechnology, Paul-Ehrlich-Institute, D-63225 Langen, Germany. |
المصدر: | Nature communications [Nat Commun] 2016 Jan 08; Vol. 7, pp. 10286. Date of Electronic Publication: 2016 Jan 08. |
نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
اللغة: | English |
بيانات الدورية: | Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101528555 Publication Model: Electronic Cited Medium: Internet ISSN: 2041-1723 (Electronic) Linking ISSN: 20411723 NLM ISO Abbreviation: Nat Commun Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: [London] : Nature Pub. Group |
مواضيع طبية MeSH: | Alu Elements/*genetics , Cell Proliferation/*genetics , Cellular Reprogramming/*genetics , Embryonic Stem Cells/*metabolism , Induced Pluripotent Stem Cells/*metabolism , Long Interspersed Nucleotide Elements/*genetics, Calcium-Binding Proteins/genetics ; Cell Line ; Cellular Reprogramming Techniques ; Epigenesis, Genetic ; Humans ; Minisatellite Repeats ; Retroelements/genetics ; Vesicular Transport Proteins/genetics |
مستخلص: | Human induced pluripotent stem cells (hiPSCs) are capable of unlimited proliferation and can differentiate in vitro to generate derivatives of the three primary germ layers. Genetic and epigenetic abnormalities have been reported by Wissing and colleagues to occur during hiPSC derivation, including mobilization of engineered LINE-1 (L1) retrotransposons. However, incidence and functional impact of endogenous retrotransposition in hiPSCs are yet to be established. Here we apply retrotransposon capture sequencing to eight hiPSC lines and three human embryonic stem cell (hESC) lines, revealing endogenous L1, Alu and SINE-VNTR-Alu (SVA) mobilization during reprogramming and pluripotent stem cell cultivation. Surprisingly, 4/7 de novo L1 insertions are full length and 6/11 retrotransposition events occurred in protein-coding genes expressed in pluripotent stem cells. We further demonstrate that an intronic L1 insertion in the CADPS2 gene is acquired during hiPSC cultivation and disrupts CADPS2 expression. These experiments elucidate endogenous retrotransposition, and its potential consequences, in hiPSCs and hESCs. |
التعليقات: | Erratum in: Nat Commun. 2018 Dec 19;9(1):5398. (PMID: 30568248) |
References: | Nature. 2011 Mar 3;471(7336):68-73. (PMID: 21289626) Nature. 2011 Mar 3;471(7336):58-62. (PMID: 21368824) Nature. 2011 Mar 3;471(7336):63-7. (PMID: 21368825) Nat Rev Cancer. 2011 Apr;11(4):268-77. (PMID: 21390058) Genome Res. 2011 Jun;21(6):985-90. (PMID: 20980553) Nat Rev Genet. 2011 Sep;12(9):615-27. (PMID: 21850042) Curr Opin Genet Dev. 2012 Jun;22(3):191-203. (PMID: 22406018) Science. 2012 Aug 24;337(6097):967-71. (PMID: 22745252) Nature. 2012 Sep 6;489(7414):75-82. (PMID: 22955617) Genome Res. 2012 Dec;22(12):2328-38. (PMID: 22968929) Proc Natl Acad Sci U S A. 2011 Dec 20;108(51):20345-50. (PMID: 21940498) Proc Natl Acad Sci U S A. 2011 Dec 20;108(51):20382-7. (PMID: 22159035) Nucleic Acids Res. 2012 Feb;40(4):1666-83. (PMID: 22053090) Cell Stem Cell. 2012 Mar 2;10(3):337-44. (PMID: 22385660) Cell Stem Cell. 2012 Jun 14;10(6):678-84. (PMID: 22704507) Nat Genet. 2002 Jun;31(2):159-65. (PMID: 12006980) Cell. 2002 Aug 9;110(3):327-38. (PMID: 12176320) Nat Biotechnol. 2002 Sep;20(9):933-6. (PMID: 12161760) EMBO J. 2002 Nov 1;21(21):5899-910. (PMID: 12411507) RNA. 2003 May;9(5):607-17. (PMID: 12702819) Proc Natl Acad Sci U S A. 2003 Apr 29;100(9):5280-5. (PMID: 12682288) Nature. 2004 May 20;429(6989):268-74. (PMID: 15152245) Nature. 2004 Sep 2;431(7004):96-9. (PMID: 15318244) Int J Cancer. 1980 Sep 15;26(3):269-80. (PMID: 6169654) EMBO J. 1984 Aug;3(8):1753-9. (PMID: 6090124) Cell. 1993 Feb 26;72(4):595-605. (PMID: 7679954) Cell. 1996 Nov 29;87(5):917-27. (PMID: 8945518) Proc Natl Acad Sci U S A. 1997 Mar 4;94(5):1872-7. (PMID: 9050872) Nat Genet. 1997 May;16(1):37-43. (PMID: 9140393) Science. 1998 Nov 6;282(5391):1145-7. (PMID: 9804556) Hum Mol Genet. 1999 Aug;8(8):1557-60. (PMID: 10401005) Genome Res. 2005 Jun;15(6):780-9. (PMID: 15930490) Mol Cell Biol. 2005 Sep;25(17):7780-95. (PMID: 16107723) J Mol Biol. 2005 Dec 9;354(4):994-1007. (PMID: 16288912) Hum Mutat. 2006 Apr;27(4):323-9. (PMID: 16511833) Hum Mol Genet. 2015 Oct 15;24(20):5759-74. (PMID: 26231220) Annu Rev Genomics Hum Genet. 2011;12:187-215. (PMID: 21801021) Cell Stem Cell. 2011 Oct 4;9(4):366-73. (PMID: 21982236) J Biol Chem. 2011 Oct 21;286(42):36427-37. (PMID: 21878639) Nature. 2011 Nov 24;479(7374):534-7. (PMID: 22037309) Hum Mol Genet. 2012 Jan 1;21(1):208-18. (PMID: 21989055) Nature. 2012 Dec 20;492(7429):438-42. (PMID: 23160490) Nucleic Acids Res. 2013 Feb 1;41(3):1829-47. (PMID: 23275558) Stem Cells. 2013 Mar;31(3):467-78. (PMID: 23225669) Gene. 2013 Apr 15;518(2):231-41. (PMID: 23333607) Cell. 2013 Mar 28;153(1):101-11. (PMID: 23540693) Nature. 2013 Nov 28;503(7477):525-9. (PMID: 24153179) Am J Pathol. 2014 May;184(5):1280-6. (PMID: 24607009) Genome Res. 2014 Aug;24(8):1251-9. (PMID: 24879558) PLoS One. 2014;9(10):e108682. (PMID: 25289675) Nucleic Acids Res. 2015 Jan;43(Database issue):D43-7. (PMID: 25352549) Cell. 2015 Apr 9;161(2):228-39. (PMID: 25860606) PLoS Genet. 2015 May;11(5):e1005121. (PMID: 25951186) Anal Biochem. 2000 Oct 15;285(2):194-204. (PMID: 11017702) Nucleic Acids Res. 2001 Jan 15;29(2):573-7. (PMID: 11139628) Annu Rev Genet. 2001;35:501-38. (PMID: 11700292) Genome Res. 2001 Dec;11(12):2059-65. (PMID: 11731496) Science. 2002 Feb 8;295(5557):1083-6. (PMID: 11834838) Trends Genet. 2007 Apr;23(4):183-91. (PMID: 17331616) Science. 2007 Jun 8;316(5830):1484-8. (PMID: 17510325) Hum Mol Genet. 2007 Jul 1;16(13):1569-77. (PMID: 17468180) Hum Mol Genet. 2007 Jul 1;16(13):1587-92. (PMID: 17483097) Mol Cell Biol. 2007 Sep;27(18):6469-83. (PMID: 17562864) Cell Stem Cell. 2007 Jun 7;1(1):55-70. (PMID: 18371336) Genes Dev. 2009 Jun 1;23(11):1303-12. (PMID: 19487571) Nature. 2009 Aug 27;460(7259):1127-31. (PMID: 19657334) Cell Stem Cell. 2009 Oct 2;5(4):434-41. (PMID: 19796623) Cell. 2010 Jun 25;141(7):1159-70. (PMID: 20602998) Cell. 2010 Jun 25;141(7):1253-61. (PMID: 20603005) Cell Stem Cell. 2010 Aug 6;7(2):249-57. (PMID: 20682450) Genome Res. 2010 Sep;20(9):1262-70. (PMID: 20488934) Cell Stem Cell. 2010 Oct 8;7(4):521-31. (PMID: 20887957) PLoS Genet. 2010 Oct;6(10). pii: e1001150. doi: 10.1371/journal.pgen.1001150. (PMID: 20949108) Cell Stem Cell. 2011 Jan 7;8(1):106-18. (PMID: 21211785) |
معلومات مُعتمدة: | IECS-55007420 United States Howard Hughes Medical Institute |
المشرفين على المادة: | 0 (CADPS2 protein, human) 0 (Calcium-Binding Proteins) 0 (Retroelements) 0 (Vesicular Transport Proteins) |
تواريخ الأحداث: | Date Created: 20160109 Date Completed: 20160517 Latest Revision: 20220330 |
رمز التحديث: | 20221213 |
مُعرف محوري في PubMed: | PMC4729875 |
DOI: | 10.1038/ncomms10286 |
PMID: | 26743714 |
قاعدة البيانات: | MEDLINE |
تدمد: | 2041-1723 |
---|---|
DOI: | 10.1038/ncomms10286 |