دورية أكاديمية
أعرض في EDS

Reprogramming triggers endogenous L1 and Alu retrotransposition in human induced pluripotent stem cells.

التفاصيل البيبلوغرافية
العنوان: Reprogramming triggers endogenous L1 and Alu retrotransposition in human induced pluripotent stem cells.
المؤلفون: Klawitter S; Division of Medical Biotechnology, Paul-Ehrlich-Institute, D-63225 Langen, Germany., Fuchs NV; Division of Medical Biotechnology, Paul-Ehrlich-Institute, D-63225 Langen, Germany.; Max-Delbrück-Center for Molecular Medicine, D-13125 Berlin, Germany., Upton KR; Mater Research Institute, University of Queensland, TRI Building, Woolloongabba, Brisbane, Queensland 4102, Australia., Muñoz-Lopez M; Department of Human DNA Variability, Pfizer/University of Granada and Andalusian Regional Government Center for Genomics and Oncology (GENYO), PTS Granada, 18016 Granada, Spain., Shukla R; Mater Research Institute, University of Queensland, TRI Building, Woolloongabba, Brisbane, Queensland 4102, Australia., Wang J; Max-Delbrück-Center for Molecular Medicine, D-13125 Berlin, Germany., Garcia-Cañadas M; Department of Human DNA Variability, Pfizer/University of Granada and Andalusian Regional Government Center for Genomics and Oncology (GENYO), PTS Granada, 18016 Granada, Spain., Lopez-Ruiz C; Department of Human DNA Variability, Pfizer/University of Granada and Andalusian Regional Government Center for Genomics and Oncology (GENYO), PTS Granada, 18016 Granada, Spain., Gerhardt DJ; Mater Research Institute, University of Queensland, TRI Building, Woolloongabba, Brisbane, Queensland 4102, Australia., Sebe A; Division of Medical Biotechnology, Paul-Ehrlich-Institute, D-63225 Langen, Germany., Grabundzija I; Max-Delbrück-Center for Molecular Medicine, D-13125 Berlin, Germany., Merkert S; Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Department of Cardiac, Thoracic, Transplantation, and Vascular Surgery; REBIRTH, Cluster of Excellence, Hannover Medical School, D-30625 Hannover, Germany., Gerdes P; Mater Research Institute, University of Queensland, TRI Building, Woolloongabba, Brisbane, Queensland 4102, Australia., Pulgarin JA; Department of Human DNA Variability, Pfizer/University of Granada and Andalusian Regional Government Center for Genomics and Oncology (GENYO), PTS Granada, 18016 Granada, Spain., Bock A; Division of Medical Biotechnology, Paul-Ehrlich-Institute, D-63225 Langen, Germany., Held U; Division of Medical Biotechnology, Paul-Ehrlich-Institute, D-63225 Langen, Germany., Witthuhn A; Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Department of Cardiac, Thoracic, Transplantation, and Vascular Surgery; REBIRTH, Cluster of Excellence, Hannover Medical School, D-30625 Hannover, Germany., Haase A; Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Department of Cardiac, Thoracic, Transplantation, and Vascular Surgery; REBIRTH, Cluster of Excellence, Hannover Medical School, D-30625 Hannover, Germany., Sarkadi B; Department of Biophysics and Radiation Biology, Semmelweis University, H-1094 Budapest, Hungary., Löwer J; Division of Medical Biotechnology, Paul-Ehrlich-Institute, D-63225 Langen, Germany., Wolvetang EJ; Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, St Lucia, Queensland 4072, Australia., Martin U; Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Department of Cardiac, Thoracic, Transplantation, and Vascular Surgery; REBIRTH, Cluster of Excellence, Hannover Medical School, D-30625 Hannover, Germany., Ivics Z; Division of Medical Biotechnology, Paul-Ehrlich-Institute, D-63225 Langen, Germany., Izsvák Z; Max-Delbrück-Center for Molecular Medicine, D-13125 Berlin, Germany., Garcia-Perez JL; Department of Human DNA Variability, Pfizer/University of Granada and Andalusian Regional Government Center for Genomics and Oncology (GENYO), PTS Granada, 18016 Granada, Spain., Faulkner GJ; Mater Research Institute, University of Queensland, TRI Building, Woolloongabba, Brisbane, Queensland 4102, Australia.; Queensland Brain Institute, University of Queensland, Brisbane, Queensland 4072, Australia., Schumann GG; Division of Medical Biotechnology, Paul-Ehrlich-Institute, D-63225 Langen, Germany.
المصدر: Nature communications [Nat Commun] 2016 Jan 08; Vol. 7, pp. 10286. Date of Electronic Publication: 2016 Jan 08.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101528555 Publication Model: Electronic Cited Medium: Internet ISSN: 2041-1723 (Electronic) Linking ISSN: 20411723 NLM ISO Abbreviation: Nat Commun Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [London] : Nature Pub. Group
مواضيع طبية MeSH: Alu Elements/*genetics , Cell Proliferation/*genetics , Cellular Reprogramming/*genetics , Embryonic Stem Cells/*metabolism , Induced Pluripotent Stem Cells/*metabolism , Long Interspersed Nucleotide Elements/*genetics, Calcium-Binding Proteins/genetics ; Cell Line ; Cellular Reprogramming Techniques ; Epigenesis, Genetic ; Humans ; Minisatellite Repeats ; Retroelements/genetics ; Vesicular Transport Proteins/genetics
مستخلص: Human induced pluripotent stem cells (hiPSCs) are capable of unlimited proliferation and can differentiate in vitro to generate derivatives of the three primary germ layers. Genetic and epigenetic abnormalities have been reported by Wissing and colleagues to occur during hiPSC derivation, including mobilization of engineered LINE-1 (L1) retrotransposons. However, incidence and functional impact of endogenous retrotransposition in hiPSCs are yet to be established. Here we apply retrotransposon capture sequencing to eight hiPSC lines and three human embryonic stem cell (hESC) lines, revealing endogenous L1, Alu and SINE-VNTR-Alu (SVA) mobilization during reprogramming and pluripotent stem cell cultivation. Surprisingly, 4/7 de novo L1 insertions are full length and 6/11 retrotransposition events occurred in protein-coding genes expressed in pluripotent stem cells. We further demonstrate that an intronic L1 insertion in the CADPS2 gene is acquired during hiPSC cultivation and disrupts CADPS2 expression. These experiments elucidate endogenous retrotransposition, and its potential consequences, in hiPSCs and hESCs.
التعليقات: Erratum in: Nat Commun. 2018 Dec 19;9(1):5398. (PMID: 30568248)
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معلومات مُعتمدة: IECS-55007420 United States Howard Hughes Medical Institute
المشرفين على المادة: 0 (CADPS2 protein, human)
0 (Calcium-Binding Proteins)
0 (Retroelements)
0 (Vesicular Transport Proteins)
تواريخ الأحداث: Date Created: 20160109 Date Completed: 20160517 Latest Revision: 20220330
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC4729875
DOI: 10.1038/ncomms10286
PMID: 26743714
قاعدة البيانات: MEDLINE
الوصف
تدمد:2041-1723
DOI:10.1038/ncomms10286