دورية أكاديمية
أعرض في EDS

Sperm Oxidative Stress Is Detrimental to Embryo Development: A Dose-Dependent Study Model and a New and More Sensitive Oxidative Status Evaluation.

التفاصيل البيبلوغرافية
العنوان: Sperm Oxidative Stress Is Detrimental to Embryo Development: A Dose-Dependent Study Model and a New and More Sensitive Oxidative Status Evaluation.
المؤلفون: de Castro LS; Laboratory of Spermatozoa Biology, Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of Sao Paulo, Avenida Prof. Dr. Orlando Marques de Paiva 87, Cidade Universitária, 05508-270 Sao Paulo, SP, Brazil., de Assis PM; Laboratory of Spermatozoa Biology, Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of Sao Paulo, Avenida Prof. Dr. Orlando Marques de Paiva 87, Cidade Universitária, 05508-270 Sao Paulo, SP, Brazil., Siqueira AF; Laboratory of Spermatozoa Biology, Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of Sao Paulo, Avenida Prof. Dr. Orlando Marques de Paiva 87, Cidade Universitária, 05508-270 Sao Paulo, SP, Brazil., Hamilton TR; Laboratory of Spermatozoa Biology, Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of Sao Paulo, Avenida Prof. Dr. Orlando Marques de Paiva 87, Cidade Universitária, 05508-270 Sao Paulo, SP, Brazil., Mendes CM; Laboratory of Spermatozoa Biology, Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of Sao Paulo, Avenida Prof. Dr. Orlando Marques de Paiva 87, Cidade Universitária, 05508-270 Sao Paulo, SP, Brazil; Laboratory of In Vitro Fertilization, Cloning and Animal Transgenesis, Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of Sao Paulo, Sao Paulo, Brazil., Losano JD; Laboratory of Andrology, Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of Sao Paulo, Sao Paulo, Brazil., Nichi M; Laboratory of Andrology, Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of Sao Paulo, Sao Paulo, Brazil., Visintin JA; Laboratory of Spermatozoa Biology, Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of Sao Paulo, Avenida Prof. Dr. Orlando Marques de Paiva 87, Cidade Universitária, 05508-270 Sao Paulo, SP, Brazil; Laboratory of In Vitro Fertilization, Cloning and Animal Transgenesis, Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of Sao Paulo, Sao Paulo, Brazil., Assumpção ME; Laboratory of Spermatozoa Biology, Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of Sao Paulo, Avenida Prof. Dr. Orlando Marques de Paiva 87, Cidade Universitária, 05508-270 Sao Paulo, SP, Brazil.
المصدر: Oxidative medicine and cellular longevity [Oxid Med Cell Longev] 2016; Vol. 2016, pp. 8213071. Date of Electronic Publication: 2015 Dec 06.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Hindawi Pub. Corp Country of Publication: United States NLM ID: 101479826 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1942-0994 (Electronic) Linking ISSN: 19420994 NLM ISO Abbreviation: Oxid Med Cell Longev Subsets: MEDLINE
أسماء مطبوعة: Publication: 2011- : New York : Hindawi Pub. Corp.
Original Publication: 2008-2010: Austin, TX : Landes Bioscience
مواضيع طبية MeSH: Embryonic Development*/drug effects , Oxidative Stress*/drug effects, Spermatozoa/*pathology, Animals ; Cattle ; Green Fluorescent Proteins/metabolism ; Hydrogen Peroxide/pharmacology ; Male ; Models, Biological ; Oxidation-Reduction/drug effects ; Sperm Motility/drug effects ; Spermatozoa/drug effects
مستخلص: Our study aimed to assess the impact of sperm oxidative stress on embryo development by means of a dose-dependent model. In experiment 1, straws from five bulls were subjected to incubation with increasing H2O2 doses (0, 12.5, 25, and 50 μM). Motility parameters were evaluated by Computed Assisted System Analysis (CASA). Experiment 2 was designed to study a high (50 μM) and low dose (12.5 μM) of H2O2 compared to a control (0 μM). Samples were incubated and further used for in vitro fertilization. Analyses of motility (CASA), oxidative status (CellROX green and 2'-7' dichlorofluorescein diacetate), mitochondrial potential (JC-1), chromatin integrity (AO), and sperm capacitation status (chlortetracycline) were performed. Embryos were evaluated based on fast cleavage (30 h.p.i.), cleavage (D = 3), development (D = 5), and blastocyst rates (D = 8). We observed a dose-dependent deleterious effect of H2O2 on motility and increase on the percentages of positive cells for CellROX green, capacitated sperm, and AO. A decrease on cleavage and blastocyst rates was observed as H2O2 increased. Also, we detected a blockage on embryo development. We concluded that sperm when exposed to oxidative environment presents impaired motility traits, prooxidative status, and premature capacitation; such alterations resulting in embryo development fail.
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المشرفين على المادة: 147336-22-9 (Green Fluorescent Proteins)
BBX060AN9V (Hydrogen Peroxide)
تواريخ الأحداث: Date Created: 20160116 Date Completed: 20161014 Latest Revision: 20181113
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC4684862
DOI: 10.1155/2016/8213071
PMID: 26770658
قاعدة البيانات: MEDLINE
الوصف
تدمد:1942-0994
DOI:10.1155/2016/8213071