دورية أكاديمية
أعرض في EDS

Aldosterone alters the chromatin structure of the murine endothelin-1 gene.

التفاصيل البيبلوغرافية
العنوان: Aldosterone alters the chromatin structure of the murine endothelin-1 gene.
المؤلفون: Welch AK; North Florida/South Georgia VA Health System, Gainesville, FL 32608, United States; Department of Physiology, University of Florida, Gainesville, FL 32608, United States., Jeanette Lynch I; North Florida/South Georgia VA Health System, Gainesville, FL 32608, United States; Department of Medicine, Division of Nephrology, Hypertension, and Renal Transplantation, University of Florida, Gainesville, FL 32608, United States., Gumz ML; Department of Medicine, Division of Nephrology, Hypertension, and Renal Transplantation, University of Florida, Gainesville, FL 32608, United States; Department of Biochemistry and Molecular Biology, University of Florida, Gainesville, FL 32608, United States., Cain BD; Department of Biochemistry and Molecular Biology, University of Florida, Gainesville, FL 32608, United States., Wingo CS; North Florida/South Georgia VA Health System, Gainesville, FL 32608, United States; Department of Medicine, Division of Nephrology, Hypertension, and Renal Transplantation, University of Florida, Gainesville, FL 32608, United States. Electronic address: cswingo@ufl.edu.
المصدر: Life sciences [Life Sci] 2016 Aug 15; Vol. 159, pp. 121-126. Date of Electronic Publication: 2016 Jan 14.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 0375521 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0631 (Electronic) Linking ISSN: 00243205 NLM ISO Abbreviation: Life Sci Subsets: MEDLINE
أسماء مطبوعة: Publication: <2008->: Amsterdam : Elsevier
Original Publication: Oxford; Elmsford, N. Y. [etc.] Pergamon Press.
مواضيع طبية MeSH: Aldosterone/*pharmacology , Chromatin/*drug effects , Endothelin-1/*genetics, Animals ; Cell Line ; Chromatin/chemistry ; Mice ; Protein Conformation ; Transcription, Genetic/drug effects
مستخلص: Unlabelled: Aldosterone increases sodium reabsorption in the renal collecting duct and systemic blood pressure. Paradoxically, aldosterone also induces transcription of the endothelin-1 (Edn1) gene to increase protein (ET-1) levels, which inhibits sodium reabsorption.
Aims: Here we investigated changes in the chromatin structure of the Edn1 gene of collecting duct cell lines in response to aldosterone treatment. The Edn1 gene has a CpG island that encompasses the transcription start site and four sites in the 5' regulatory region previously linked to transcriptional regulation.
Materials and Methods: The chromatin structure of the Edn1 gene was investigated using a quantitative PCR-based DNaseI hypersensitivity assay in murine hepatocyte (AML12), renal cortical collecting duct (mpkCCDC14), outer medullary collecting duct1 (OMCD1), and inner medullary collecting duct-3 (IMCD-3) cell lines.
Key Findings: The CpG island was uniformly accessible. One calcium-responsive NFAT element remained at low chromatin accessibility in all cell lines under all conditions tested. However, the second calcium responsive NFAT element located at -1563bp upstream became markedly more accessible in IMCD-3 cells exposed to aldosterone. Importantly, one established aldosterone hormone response element HRE at -671bp relative to the transcription start site was highly accessible, and another HRE (-551bp) became more accessible in aldosterone-treated IMCD-3 and OMCD1 cells.
Significance: The evidence supports a model in which aldosterone activation of the mineralocorticoid receptor (MR) results in the MR-hormone complex binding at HRE at -671bp to open chromatin structure around other regulatory elements in the Edn1 gene.
(Published by Elsevier Inc.)
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معلومات مُعتمدة: I01 BX001472 United States BX BLRD VA; R01 DK082680 United States DK NIDDK NIH HHS; T32 HL083810 United States HL NHLBI NIH HHS
فهرسة مساهمة: Keywords: Aldosterone; Chromatin; Chromatin structure; Epigenetics; Kidney
المشرفين على المادة: 0 (Chromatin)
0 (Endothelin-1)
4964P6T9RB (Aldosterone)
تواريخ الأحداث: Date Created: 20160119 Date Completed: 20170208 Latest Revision: 20181113
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC4945465
DOI: 10.1016/j.lfs.2016.01.019
PMID: 26775567
قاعدة البيانات: MEDLINE
الوصف
تدمد:1879-0631
DOI:10.1016/j.lfs.2016.01.019