دورية أكاديمية
Aldosterone alters the chromatin structure of the murine endothelin-1 gene.
العنوان: | Aldosterone alters the chromatin structure of the murine endothelin-1 gene. |
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المؤلفون: | Welch AK; North Florida/South Georgia VA Health System, Gainesville, FL 32608, United States; Department of Physiology, University of Florida, Gainesville, FL 32608, United States., Jeanette Lynch I; North Florida/South Georgia VA Health System, Gainesville, FL 32608, United States; Department of Medicine, Division of Nephrology, Hypertension, and Renal Transplantation, University of Florida, Gainesville, FL 32608, United States., Gumz ML; Department of Medicine, Division of Nephrology, Hypertension, and Renal Transplantation, University of Florida, Gainesville, FL 32608, United States; Department of Biochemistry and Molecular Biology, University of Florida, Gainesville, FL 32608, United States., Cain BD; Department of Biochemistry and Molecular Biology, University of Florida, Gainesville, FL 32608, United States., Wingo CS; North Florida/South Georgia VA Health System, Gainesville, FL 32608, United States; Department of Medicine, Division of Nephrology, Hypertension, and Renal Transplantation, University of Florida, Gainesville, FL 32608, United States. Electronic address: cswingo@ufl.edu. |
المصدر: | Life sciences [Life Sci] 2016 Aug 15; Vol. 159, pp. 121-126. Date of Electronic Publication: 2016 Jan 14. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 0375521 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0631 (Electronic) Linking ISSN: 00243205 NLM ISO Abbreviation: Life Sci Subsets: MEDLINE |
أسماء مطبوعة: | Publication: <2008->: Amsterdam : Elsevier Original Publication: Oxford; Elmsford, N. Y. [etc.] Pergamon Press. |
مواضيع طبية MeSH: | Aldosterone/*pharmacology , Chromatin/*drug effects , Endothelin-1/*genetics, Animals ; Cell Line ; Chromatin/chemistry ; Mice ; Protein Conformation ; Transcription, Genetic/drug effects |
مستخلص: | Unlabelled: Aldosterone increases sodium reabsorption in the renal collecting duct and systemic blood pressure. Paradoxically, aldosterone also induces transcription of the endothelin-1 (Edn1) gene to increase protein (ET-1) levels, which inhibits sodium reabsorption. Aims: Here we investigated changes in the chromatin structure of the Edn1 gene of collecting duct cell lines in response to aldosterone treatment. The Edn1 gene has a CpG island that encompasses the transcription start site and four sites in the 5' regulatory region previously linked to transcriptional regulation. Materials and Methods: The chromatin structure of the Edn1 gene was investigated using a quantitative PCR-based DNaseI hypersensitivity assay in murine hepatocyte (AML12), renal cortical collecting duct (mpkCCDC14), outer medullary collecting duct1 (OMCD1), and inner medullary collecting duct-3 (IMCD-3) cell lines. Key Findings: The CpG island was uniformly accessible. One calcium-responsive NFAT element remained at low chromatin accessibility in all cell lines under all conditions tested. However, the second calcium responsive NFAT element located at -1563bp upstream became markedly more accessible in IMCD-3 cells exposed to aldosterone. Importantly, one established aldosterone hormone response element HRE at -671bp relative to the transcription start site was highly accessible, and another HRE (-551bp) became more accessible in aldosterone-treated IMCD-3 and OMCD1 cells. Significance: The evidence supports a model in which aldosterone activation of the mineralocorticoid receptor (MR) results in the MR-hormone complex binding at HRE at -671bp to open chromatin structure around other regulatory elements in the Edn1 gene. (Published by Elsevier Inc.) |
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معلومات مُعتمدة: | I01 BX001472 United States BX BLRD VA; R01 DK082680 United States DK NIDDK NIH HHS; T32 HL083810 United States HL NHLBI NIH HHS |
فهرسة مساهمة: | Keywords: Aldosterone; Chromatin; Chromatin structure; Epigenetics; Kidney |
المشرفين على المادة: | 0 (Chromatin) 0 (Endothelin-1) 4964P6T9RB (Aldosterone) |
تواريخ الأحداث: | Date Created: 20160119 Date Completed: 20170208 Latest Revision: 20181113 |
رمز التحديث: | 20221213 |
مُعرف محوري في PubMed: | PMC4945465 |
DOI: | 10.1016/j.lfs.2016.01.019 |
PMID: | 26775567 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1879-0631 |
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DOI: | 10.1016/j.lfs.2016.01.019 |