دورية أكاديمية
Use of Serial Quantitative PCR of the vapA Gene of Rhodococcus equi in Feces for Early Detection of R. equi Pneumonia in Foals.
| العنوان: | Use of Serial Quantitative PCR of the vapA Gene of Rhodococcus equi in Feces for Early Detection of R. equi Pneumonia in Foals. |
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| المؤلفون: | Madrigal RG; Department of Large Animal Clinical Sciences, College of Biomedical Sciences and Veterinary Medicine, Texas A&M University, College Station, TX., Shaw SD; Department of Large Animal Clinical Sciences, College of Biomedical Sciences and Veterinary Medicine, Texas A&M University, College Station, TX., Witkowski LA; Laboratory of Veterinary Epidemiology and Economics, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, Warsaw, Poland., Sisson BE; Department of Large Animal Clinical Sciences, College of Biomedical Sciences and Veterinary Medicine, Texas A&M University, College Station, TX., Blodgett GP; Equine Division, 6666 Ranch, Guthrie, TX., Chaffin MK; Department of Large Animal Clinical Sciences, College of Biomedical Sciences and Veterinary Medicine, Texas A&M University, College Station, TX., Cohen ND; Department of Large Animal Clinical Sciences, College of Biomedical Sciences and Veterinary Medicine, Texas A&M University, College Station, TX. |
| المصدر: | Journal of veterinary internal medicine [J Vet Intern Med] 2016 Mar-Apr; Vol. 30 (2), pp. 664-70. Date of Electronic Publication: 2016 Jan 24. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Wiley Periodicals Country of Publication: United States NLM ID: 8708660 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1939-1676 (Electronic) Linking ISSN: 08916640 NLM ISO Abbreviation: J Vet Intern Med Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2010-> : Malden, MA : Wiley Periodicals Original Publication: Philadelphia : J.B. Lippincott Co., [c1987- |
| مواضيع طبية MeSH: | Bacterial Proteins/*isolation & purification , Feces/*microbiology , Horse Diseases/*microbiology , Pneumonia, Bacterial/*veterinary , Polymerase Chain Reaction/*veterinary , Rhodococcus equi/*isolation & purification, Actinomycetales Infections/diagnosis ; Actinomycetales Infections/microbiology ; Actinomycetales Infections/veterinary ; Animals ; Horse Diseases/diagnosis ; Horses ; Pneumonia, Bacterial/diagnosis ; Pneumonia, Bacterial/microbiology ; Polymerase Chain Reaction/methods ; Sensitivity and Specificity |
| مستخلص: | Background: Current screening tests for Rhodococcus equi pneumonia in foals lack adequate accuracy for clinical use. Real-time, quantitative PCR (qPCR) for virulent R. equi in feces has not been systematically evaluated as a screening test. Objective: The objective of this study was to evaluate the accuracy of qPCR for vapA in serially collected fecal samples as a screening test for R. equi pneumonia in foals. Animals: One hundred and twenty-five foals born in 2011 at a ranch in Texas. Methods: Fecal samples were collected concurrently with thoracic ultrasonography (TUS) screening examinations at ages 3, 5, and 7 weeks. Affected (pneumonic) foals (n = 25) were matched by age and date-of-birth to unaffected (n = 25) and subclinical (ie, having thoracic TUS lesions but no clinical signs of pneumonia) foals (n = 75). DNA was extracted from feces using commercial kits and concentration of virulent R. equi in feces was determined by qPCR. Results: Subsequently affected foals had significantly greater concentrations of vapA in feces than foals that did not develop pneumonia (unaffected and subclinical foals) at 5 and 7 weeks of age. Accuracy of fecal qPCR, however, was poor as a screening test to differentiate foals that would develop clinical signs of pneumonia from those that would remain free of clinical signs (including foals with subclinical pulmonary lesions attributed to R. equi) using receiver operating characteristic (ROC) methods. Conclusions and Clinical Importance: In the population studied, serial qPCR on feces lacked adequate accuracy as a screening test for clinical R. equi foal pneumonia. (Copyright © 2016 The Authors. Journal of Veterinary Internal Medicine published by Wiley Periodicals, Inc. on behalf of the American College of Veterinary Internal Medicine.) |
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| فهرسة مساهمة: | Keywords: Feces; Horse; Pneumonia; Rhodococcus equi |
| المشرفين على المادة: | 0 (Bacterial Proteins) 0 (VapA protein, Rhodococcus equi) |
| تواريخ الأحداث: | Date Created: 20160126 Date Completed: 20161222 Latest Revision: 20181113 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC4913589 |
| DOI: | 10.1111/jvim.13828 |
| PMID: | 26806422 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1939-1676 |
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| DOI: | 10.1111/jvim.13828 |