دورية أكاديمية
أعرض في EDS

Cause and Consequence of Tethering a SubTAD to Different Nuclear Compartments.

التفاصيل البيبلوغرافية
العنوان: Cause and Consequence of Tethering a SubTAD to Different Nuclear Compartments.
المؤلفون: Wijchers PJ; Hubrecht Institute-KNAW & University Medical Center Utrecht, Uppsalalaan 8, 3584 CT Utrecht, the Netherlands., Krijger PHL; Hubrecht Institute-KNAW & University Medical Center Utrecht, Uppsalalaan 8, 3584 CT Utrecht, the Netherlands., Geeven G; Hubrecht Institute-KNAW & University Medical Center Utrecht, Uppsalalaan 8, 3584 CT Utrecht, the Netherlands., Zhu Y; Hubrecht Institute-KNAW & University Medical Center Utrecht, Uppsalalaan 8, 3584 CT Utrecht, the Netherlands., Denker A; Hubrecht Institute-KNAW & University Medical Center Utrecht, Uppsalalaan 8, 3584 CT Utrecht, the Netherlands., Verstegen MJAM; Hubrecht Institute-KNAW & University Medical Center Utrecht, Uppsalalaan 8, 3584 CT Utrecht, the Netherlands., Valdes-Quezada C; Hubrecht Institute-KNAW & University Medical Center Utrecht, Uppsalalaan 8, 3584 CT Utrecht, the Netherlands., Vermeulen C; Hubrecht Institute-KNAW & University Medical Center Utrecht, Uppsalalaan 8, 3584 CT Utrecht, the Netherlands., Janssen M; Hubrecht Institute-KNAW & University Medical Center Utrecht, Uppsalalaan 8, 3584 CT Utrecht, the Netherlands., Teunissen H; Hubrecht Institute-KNAW & University Medical Center Utrecht, Uppsalalaan 8, 3584 CT Utrecht, the Netherlands., Anink-Groenen LCM; Synthetic Systems Biology and Nuclear Organization Group, Swammerdam Institute for Life Sciences, University of Amsterdam, 1098 XH Amsterdam, the Netherlands., Verschure PJ; Synthetic Systems Biology and Nuclear Organization Group, Swammerdam Institute for Life Sciences, University of Amsterdam, 1098 XH Amsterdam, the Netherlands., de Laat W; Hubrecht Institute-KNAW & University Medical Center Utrecht, Uppsalalaan 8, 3584 CT Utrecht, the Netherlands. Electronic address: w.delaat@hubrecht.eu.
المصدر: Molecular cell [Mol Cell] 2016 Feb 04; Vol. 61 (3), pp. 461-473. Date of Electronic Publication: 2016 Jan 28.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Cell Press Country of Publication: United States NLM ID: 9802571 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1097-4164 (Electronic) Linking ISSN: 10972765 NLM ISO Abbreviation: Mol Cell Subsets: MEDLINE
أسماء مطبوعة: Publication: Cambridge Ma : Cell Press
Original Publication: Cambridge, Mass. : Cell Press, c1997-
مواضيع طبية MeSH: Chromosome Segregation* , Genetic Loci* , Lac Operon*, Cell Nucleus/*metabolism , Embryonic Stem Cells/*metabolism , Lac Repressors/*metabolism, Animals ; Cells, Cultured ; Chromatin/metabolism ; Chromatin Assembly and Disassembly ; Enhancer of Zeste Homolog 2 Protein ; Gene Expression Regulation ; Homeodomain Proteins/genetics ; Homeodomain Proteins/metabolism ; Lac Repressors/genetics ; Methyltransferases/genetics ; Methyltransferases/metabolism ; Mice, 129 Strain ; Mice, Inbred C57BL ; Nanog Homeobox Protein ; Polycomb Repressive Complex 2/genetics ; Polycomb Repressive Complex 2/metabolism ; Repressor Proteins/genetics ; Repressor Proteins/metabolism ; Transfection
مستخلص: Detailed genomic contact maps have revealed that chromosomes are structurally organized in megabase-sized topologically associated domains (TADs) that encompass smaller subTADs. These domains segregate in the nuclear space to form active and inactive nuclear compartments, but cause and consequence of compartmentalization are largely unknown. Here, we combined lacO/lacR binding platforms with allele-specific 4C technologies to track their precise position in the three-dimensional genome upon recruitment of NANOG, SUV39H1, or EZH2. We observed locked genomic loci resistant to spatial repositioning and unlocked loci that could be repositioned to different nuclear subcompartments with distinct chromatin signatures. Focal protein recruitment caused the entire subTAD, but not surrounding regions, to engage in new genomic contacts. Compartment switching was found uncoupled from transcription changes, and the enzymatic modification of histones per se was insufficient for repositioning. Collectively, this suggests that trans-associated factors influence three-dimensional compartmentalization independent of their cis effect on local chromatin composition and activity.
(Copyright © 2016 The Authors. Published by Elsevier Inc. All rights reserved.)
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المشرفين على المادة: 0 (Chromatin)
0 (Homeodomain Proteins)
0 (Lac Repressors)
0 (Nanog Homeobox Protein)
0 (Nanog protein, mouse)
0 (Repressor Proteins)
EC 2.1.1. (Suv39h1 protein, mouse)
EC 2.1.1.- (Methyltransferases)
EC 2.1.1.43 (Enhancer of Zeste Homolog 2 Protein)
EC 2.1.1.43 (Ezh2 protein, mouse)
EC 2.1.1.43 (Polycomb Repressive Complex 2)
تواريخ الأحداث: Date Created: 20160203 Date Completed: 20160629 Latest Revision: 20240524
رمز التحديث: 20240524
مُعرف محوري في PubMed: PMC4747903
DOI: 10.1016/j.molcel.2016.01.001
PMID: 26833089
قاعدة البيانات: MEDLINE
الوصف
تدمد:1097-4164
DOI:10.1016/j.molcel.2016.01.001