دورية أكاديمية
[THE EFFECT OF waaL LIGASE GENES DELETION ON MOTILITY AND STRESS ADAPTATION REACTIONS OF YERSINIA ENTEROCOLITICA 6471/76].
| العنوان: | [THE EFFECT OF waaL LIGASE GENES DELETION ON MOTILITY AND STRESS ADAPTATION REACTIONS OF YERSINIA ENTEROCOLITICA 6471/76]. |
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| المؤلفون: | Shevchenko JI, Shilina JV, Pozur VK, Skurnik M |
| المصدر: | TSitologiia i genetika [Tsitol Genet] 2015 Nov-Dec; Vol. 49 (6), pp. 13-20. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | Russian |
| بيانات الدورية: | Publisher: Izdatelstvo Naukova Dumka Country of Publication: Ukraine NLM ID: 0101671 Publication Model: Print Cited Medium: Print ISSN: 0564-3783 (Print) Linking ISSN: 05643783 NLM ISO Abbreviation: Tsitol Genet Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Kiev : Izdatelstvo Naukova Dumka Original Publication: Kiev. |
| مواضيع طبية MeSH: | Gene Deletion*, Adaptation, Physiological/*genetics , Carbon-Oxygen Ligases/*genetics , Lipopolysaccharides/*biosynthesis , Stress, Physiological/*genetics , Yersinia enterocolitica/*enzymology, Bacterial Physiological Phenomena/genetics ; Genetic Complementation Test ; Mutation ; Osmotic Pressure/physiology ; Yersinia enterocolitica/genetics ; Yersinia enterocolitica/physiology |
| مستخلص: | The aim of current study was to estimate the influence of waaL(OS) and waaL(PS) genes deletion on lipopolysaccharide (LPS) synthesis, bacterial motility and stress resistance of bacteria Yersinia enterocolitica 6471/76. Single and double waaL mutants were created by replacing the wild-type alleles in bacterial chromosome for mutant ones. The phenotypes of mutants were visualized by DOC-PAGE gels stained with silver and immunoblot with specific to O-polysaccharide and outer core monoclonal antibodies. Bacterial motility was evaluated by the diameter of the migration zone. Wild type bacteria and mutants were analyzed by bacterial growth curves in a hypertonic medium. Participation of WaaL ligases in resistance to osmotic pressure was found only in case of both ligese genes deletion. Also the YeO3_os_ps mutants showed motility decreasing, which recovered after adding a functionally active gene. Thus, deletion of both waaL ligase genes lead to a drastic reduction in bacterial motility and increase their sensitivity to hypertonic medium that can indirectly characterize biological role of WaaL ligases. |
| المشرفين على المادة: | 0 (Lipopolysaccharides) EC 6.1.- (Carbon-Oxygen Ligases) |
| تواريخ الأحداث: | Date Created: 20160205 Date Completed: 20160301 Latest Revision: 20161126 |
| رمز التحديث: | 20231215 |
| PMID: | 26841489 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0564-3783 |
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