دورية أكاديمية
Stem cell-derived tissue-associated regulatory T cells ameliorate the development of autoimmunity.
العنوان: | Stem cell-derived tissue-associated regulatory T cells ameliorate the development of autoimmunity. |
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المؤلفون: | Haque M; Department of Microbiology and Immunology, The Pennsylvania State University College of Medicine, Hershey, PA 17033, USA., Song J; Institutes of Irradiation/Immunology, The Third Military Medical University, Chongqing 400038, China., Fino K; Department of Microbiology and Immunology, The Pennsylvania State University College of Medicine, Hershey, PA 17033, USA., Sandhu P; Department of Microbiology and Immunology, The Pennsylvania State University College of Medicine, Hershey, PA 17033, USA., Song X; Department of Microbiology and Immunology, The Pennsylvania State University College of Medicine, Hershey, PA 17033, USA., Lei F; Department of Microbiology and Immunology, The Pennsylvania State University College of Medicine, Hershey, PA 17033, USA., Zheng S; Department of Medicine, The Pennsylvania State University College of Medicine, Hershey, PA 17033, USA., Ni B; Institutes of Irradiation/Immunology, The Third Military Medical University, Chongqing 400038, China., Fang D; Department of Pathology, Northwestern University Feinberg School of Medicine, Chicago, IL 60611, USA., Song J; Department of Microbiology and Immunology, The Pennsylvania State University College of Medicine, Hershey, PA 17033, USA. |
المصدر: | Scientific reports [Sci Rep] 2016 Feb 05; Vol. 6, pp. 20588. Date of Electronic Publication: 2016 Feb 05. |
نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
اللغة: | English |
بيانات الدورية: | Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: London : Nature Publishing Group, copyright 2011- |
مواضيع طبية MeSH: | Autoimmunity*, Adoptive Transfer/*methods , Arthritis, Experimental/*therapy , Induced Pluripotent Stem Cells/*cytology , T-Lymphocytes, Regulatory/*cytology, Animals ; Arthritis, Experimental/immunology ; Cell Line ; Cells, Cultured ; Coculture Techniques ; Disease Models, Animal ; Forkhead Transcription Factors/genetics ; Forkhead Transcription Factors/metabolism ; Mice ; Receptors, Antigen, T-Cell/genetics ; Receptors, Antigen, T-Cell/metabolism ; T-Lymphocytes, Regulatory/immunology ; Transduction, Genetic |
مستخلص: | Pluripotent stem cells (PSCs) have the potential to produce almost all of the cells in the body, including regulatory T cells (Tregs). However, the exact conditions required for the development of antigen (Ag)-specific Tregs from PSCs (i.e., PSC-Tregs) are not well delineated. Ag-specific PSC-Tregs can be tissue/organ-associated and migrate to local inflamed tissues/organs to suppress the autoimmune response after adoptive transfer, thereby avoiding potential overall immunosuppression from non-specific Tregs. In this study, we developed a new approach to generate functional Ag-specific Tregs from induced PSCs (iPSCs), i.e., iPSC-Tregs, which had the ability to generate an Ag-specific immunosuppressive response in a murine model of arthritis. We retrovirally transduced murine iPSCs with a construct containing genes of Ag-specific T cell receptor (TCR) and the transcriptional factor FoxP3. We differentiated the iPSCs into Ag-specific iPSC-Tregs using in vitro or in vivo Notch signaling, and demonstrated that adoptive transfer of such Tregs dramatically suppressed autoimmunity in a well-established Ag-induced arthritis model, including the inflammation, joint destruction, cartilage prostaglandin depletion, osteoclast activity, and Th17 production. Our results indicate that PSCs can be used to develop Ag-specific Tregs, which have a therapeutic potential for Treg-based therapies of autoimmune disorders. |
References: | Cancer Res. 2011 Jul 15;71(14):4742-7. (PMID: 21628492) Blood. 2007 Jan 15;109(2):827-35. (PMID: 17003369) Proc Natl Acad Sci U S A. 2012 Aug 28;109(35):14134-9. (PMID: 22891339) Hepatology. 2013 Jan;57(1):217-27. (PMID: 22911361) Immunity. 2014 Dec 18;41(6):1013-25. (PMID: 25526312) Blood. 2008 Sep 1;112(5):1813-21. (PMID: 18550850) Cancer Immunol Immunother. 2010 Jun;59(6):851-62. (PMID: 20052466) Nature. 2009 Jul 2;460(7251):53-9. (PMID: 19483674) Clin Immunol. 2014 Aug;153(2):298-307. (PMID: 24858581) Lancet. 2005 Jul 2-8;366(9479):50-6. (PMID: 15993233) Proc Natl Acad Sci U S A. 2007 Nov 13;104(46):18169-74. (PMID: 17978190) J Exp Med. 2009 Aug 3;206(8):1769-85. (PMID: 19620629) Nat Med. 2014 Jan;20(1):62-8. (PMID: 24362934) Mucosal Immunol. 2014 Jul;7(4):916-28. (PMID: 24301658) Proc Natl Acad Sci U S A. 2008 May 13;105(19):7010-5. (PMID: 18458347) Immunity. 2013 Nov 14;39(5):949-62. (PMID: 24238343) Autoimmunity. 2011 Sep;44(6):471-82. (PMID: 21370936) J Immunol. 2010 Mar 1;184(5):2261-71. (PMID: 20118279) Blood. 2012 Mar 22;119(12):2810-8. (PMID: 22294730) Mol Cell Biol. 2014 Nov;34(21):3993-4007. (PMID: 25154413) Front Oncol. 2014 Aug 08;4:209. (PMID: 25152867) J Immunol. 2008 Apr 1;180(7):4366-70. (PMID: 18354156) J Exp Med. 2011 Nov 21;208(12):2489-96. (PMID: 22084406) Arthritis Res Ther. 2012;14(2):R45. (PMID: 22390640) J Immunol. 2008 Dec 15;181(12):8209-13. (PMID: 19050237) Sci Transl Med. 2013 Dec 11;5(215):215ra174. (PMID: 24337481) Sci Transl Med. 2014 Jun 18;6(241):241ra78. (PMID: 24944193) Proc Natl Acad Sci U S A. 2009 Nov 10;106(45):19078-83. (PMID: 19884493) J Immunol. 2012 Aug 1;189(3):1228-36. (PMID: 22732595) J Vis Exp. 2012;(63):e3986. (PMID: 22617911) Proc Natl Acad Sci U S A. 2010 Jun 15;107(24):10972-7. (PMID: 20534461) Science. 2014 Dec 19;346(6216):1536-40. (PMID: 25525252) Science. 2003 Feb 14;299(5609):1057-61. (PMID: 12522256) Cell. 2009 Feb 6;136(3):411-9. (PMID: 19203577) J Exp Med. 2004 Jun 7;199(11):1455-65. (PMID: 15184499) J Immunol. 2014 Dec 15;193(12):5914-23. (PMID: 25381435) Nat Immunol. 2014 Nov;15(11):1070-8. (PMID: 25263123) Cell Stem Cell. 2013 Jan 3;12(1):31-6. (PMID: 23290135) J Immunol. 2011 Aug 15;187(4):1745-53. (PMID: 21746962) Nat Med. 2010 Apr;16(4):475-82. (PMID: 20305662) Nat Biotechnol. 2013 Oct;31(10):928-33. (PMID: 23934177) Immunity. 2007 Nov;27(5):786-800. (PMID: 18024188) Autoimmunity. 2012 Sep;45(6):449-59. (PMID: 22686732) Nat Immunol. 2015 Feb;16(2):188-96. (PMID: 25559257) Methods Mol Biol. 2014;1213:365-77. (PMID: 25173398) Blood. 2008 Feb 1;111(3):1726-34. (PMID: 18025149) Blood. 2008 Apr 15;111(8):4209-19. (PMID: 18218852) Science. 2010 Sep 24;329(5999):1667-71. (PMID: 20929851) Nature. 2011 Jun 9;474(7350):212-5. (PMID: 21572395) Arthritis Rheum. 2010 Apr;62(4):1026-35. (PMID: 20131272) Blood. 2015 Feb 12;125(7):1107-15. (PMID: 25498909) Gene Ther. 2009 Sep;16(9):1088-96. (PMID: 19554034) Cell Immunol. 2009;260(1):1-5. (PMID: 19811778) |
معلومات مُعتمدة: | R01 AI079056 United States AI NIAID NIH HHS; K18CA151798 United States CA NCI NIH HHS; R21AI109239 United States AI NIAID NIH HHS; R21 AI109239 United States AI NIAID NIH HHS; K18 CA151798 United States CA NCI NIH HHS |
المشرفين على المادة: | 0 (FOXP3 protein, human) 0 (Forkhead Transcription Factors) 0 (Receptors, Antigen, T-Cell) |
تواريخ الأحداث: | Date Created: 20160206 Date Completed: 20170104 Latest Revision: 20210102 |
رمز التحديث: | 20240628 |
مُعرف محوري في PubMed: | PMC4742827 |
DOI: | 10.1038/srep20588 |
PMID: | 26846186 |
قاعدة البيانات: | MEDLINE |
تدمد: | 2045-2322 |
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DOI: | 10.1038/srep20588 |