دورية أكاديمية
Long Interspersed Element Sequencing (L1-Seq): A Method to Identify Somatic LINE-1 Insertions in the Human Genome.
| العنوان: | Long Interspersed Element Sequencing (L1-Seq): A Method to Identify Somatic LINE-1 Insertions in the Human Genome. |
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| المؤلفون: | Doucet TT; McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, 21205, USA., Kazazian HH Jr; McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, 21205, USA. kazazian@jhmi.edu.; Department of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, MD, USA. kazazian@jhmi.edu. |
| المصدر: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2016; Vol. 1400, pp. 79-93. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural |
| اللغة: | English |
| بيانات الدورية: | Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Totowa, NJ : Humana Press Original Publication: Clifton, N.J. : Humana Press, |
| مواضيع طبية MeSH: | Genome, Human* , Genomics*/methods , Long Interspersed Nucleotide Elements*, Computational Biology/methods ; Genomic Library ; High-Throughput Nucleotide Sequencing ; Humans ; Reproducibility of Results |
| مستخلص: | L1-seq is a high-throughput sequencing technique which is utilized to identify novel L1 insertions in genomic DNA samples of interest. Using special diagnostic nucleotides unique to the youngest and most active L1 sequence, we can amplify new somatic insertions. This technique has helped to establish the number of L1 insertions present in the general population as well as the variation among individuals with regard to their complement of active L1 elements. More recently, this technique has been employed to assess the level of retrotransposition occurring in various diseases such as cancer. These efforts try to establish a connection between the process of retrotransposition and disease development and/or progression. |
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| معلومات مُعتمدة: | R01 GM099875 United States GM NIGMS NIH HHS |
| فهرسة مساهمة: | Keywords: L1; LINE-1; Next-Generation DNA sequencing; Non-LTR retrotransposon; Retroelement |
| تواريخ الأحداث: | Date Created: 20160220 Date Completed: 20161213 Latest Revision: 20220408 |
| رمز التحديث: | 20221213 |
| مُعرف محوري في PubMed: | PMC5340310 |
| DOI: | 10.1007/978-1-4939-3372-3_5 |
| PMID: | 26895047 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1940-6029 |
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| DOI: | 10.1007/978-1-4939-3372-3_5 |