دورية أكاديمية
lncRNA-Induced Nucleosome Repositioning Reinforces Transcriptional Repression of rRNA Genes upon Hypotonic Stress.
| العنوان: | lncRNA-Induced Nucleosome Repositioning Reinforces Transcriptional Repression of rRNA Genes upon Hypotonic Stress. |
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| المؤلفون: | Zhao Z; Division of Molecular Biology of the Cell II, German Cancer Research Center, DKFZ-ZMBH Alliance, Im Neuenheimer Feld 581, 69120 Heidelberg, Germany., Dammert MA; Division of Molecular Biology of the Cell II, German Cancer Research Center, DKFZ-ZMBH Alliance, Im Neuenheimer Feld 581, 69120 Heidelberg, Germany., Grummt I; Division of Molecular Biology of the Cell II, German Cancer Research Center, DKFZ-ZMBH Alliance, Im Neuenheimer Feld 581, 69120 Heidelberg, Germany., Bierhoff H; Division of Molecular Biology of the Cell II, German Cancer Research Center, DKFZ-ZMBH Alliance, Im Neuenheimer Feld 581, 69120 Heidelberg, Germany. Electronic address: holger.bierhoff@uni-jena.de. |
| المصدر: | Cell reports [Cell Rep] 2016 Mar 01; Vol. 14 (8), pp. 1876-82. Date of Electronic Publication: 2016 Feb 18. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Cell Press Country of Publication: United States NLM ID: 101573691 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2211-1247 (Electronic) NLM ISO Abbreviation: Cell Rep Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [Cambridge, MA] : Cell Press, c 2012- |
| مواضيع طبية MeSH: | Genes, rRNA* , Osmotic Pressure*, Endosomal Sorting Complexes Required for Transport/*genetics , Histone-Lysine N-Methyltransferase/*genetics , Nucleosomes/*chemistry , RNA, Long Noncoding/*genetics , Ubiquitin-Protein Ligases/*genetics, Animals ; Chromatin Assembly and Disassembly ; Endosomal Sorting Complexes Required for Transport/metabolism ; Gene Expression Regulation ; HEK293 Cells ; Histone-Lysine N-Methyltransferase/metabolism ; Histones/genetics ; Histones/metabolism ; Humans ; Mi-2 Nucleosome Remodeling and Deacetylase Complex/genetics ; Mi-2 Nucleosome Remodeling and Deacetylase Complex/metabolism ; Mice ; NIH 3T3 Cells ; Nedd4 Ubiquitin Protein Ligases ; Nucleosomes/metabolism ; Nucleosomes/ultrastructure ; Osmolar Concentration ; Proteasome Endopeptidase Complex/metabolism ; Proteolysis ; RNA, Long Noncoding/metabolism ; Signal Transduction ; Transcription, Genetic ; Ubiquitin-Protein Ligases/metabolism ; Ubiquitination |
| مستخلص: | The activity of rRNA genes (rDNA) is regulated by pathways that target the transcription machinery or alter the epigenetic state of rDNA. Previous work has established that downregulation of rRNA synthesis in quiescent cells is accompanied by upregulation of PAPAS, a long noncoding RNA (lncRNA) that recruits the histone methyltransferase Suv4-20h2 to rDNA, thus triggering trimethylation of H4K20 (H4K20me3) and chromatin compaction. Here, we show that upregulation of PAPAS in response to hypoosmotic stress does not increase H4K20me3 because of Nedd4-dependent ubiquitinylation and proteasomal degradation of Suv4-20h2. Loss of Suv4-20h2 enables PAPAS to interact with CHD4, a subunit of the chromatin remodeling complex NuRD, which shifts the promoter-bound nucleosome into the transcriptional "off" position. Thus, PAPAS exerts a "stress-tailored" dual function in rDNA silencing, facilitating either Suv4-20h2-dependent chromatin compaction or NuRD-dependent changes in nucleosome positioning. (Copyright © 2016 The Authors. Published by Elsevier Inc. All rights reserved.) |
| المشرفين على المادة: | 0 (Endosomal Sorting Complexes Required for Transport) 0 (Histones) 0 (Nucleosomes) 0 (RNA, Long Noncoding) EC 2.1.1.43 (Histone-Lysine N-Methyltransferase) EC 2.1.1.43 (KMT5C protein, human) EC 2.3.2.26 (Nedd4 Ubiquitin Protein Ligases) EC 2.3.2.26 (Nedd4 protein, human) EC 2.3.2.26 (Nedd4l protein, mouse) EC 2.3.2.27 (Ubiquitin-Protein Ligases) EC 3.4.25.1 (Proteasome Endopeptidase Complex) EC 3.5.1.98 (Mi-2 Nucleosome Remodeling and Deacetylase Complex) |
| تواريخ الأحداث: | Date Created: 20160225 Date Completed: 20161213 Latest Revision: 20191030 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1016/j.celrep.2016.01.073 |
| PMID: | 26904956 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2211-1247 |
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| DOI: | 10.1016/j.celrep.2016.01.073 |