دورية أكاديمية
Antimicrobial activity of conventional and plant-extract disinfectant solutions on microbial biofilms on a maxillofacial polymer surface.
| العنوان: | Antimicrobial activity of conventional and plant-extract disinfectant solutions on microbial biofilms on a maxillofacial polymer surface. |
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| المؤلفون: | Guiotti AM; Assistant Professor, Department of Dental Materials and Prosthodontics, Araçatuba Dental School, São Paulo State University (UNESP), São Paulo, Brazil. Electronic address: aimee@foa.unesp.br., Cunha BG; Graduate student, Department of Dental Materials and Prosthodontics, Araçatuba Dental School, São Paulo State University (UNESP), São Paulo, Brazil., Paulini MB; Graduate student, Department of Dental Materials and Prosthodontics, Araçatuba Dental School, São Paulo State University (UNESP), São Paulo, Brazil., Goiato MC; Titular Professor, Department of Dental Materials and Prosthodontics, Araçatuba Dental School, São Paulo State University (UNESP), São Paulo, Brazil., Dos Santos DM; Assistant Professor, Department of Dental Materials and Prosthodontics, Araçatuba Dental School, São Paulo State University (UNESP), São Paulo, Brazil., Duque C; Assistant Professor, Department of Pediatric and Public Health, Araçatuba Dental School, São Paulo State University (UNESP), Araçatuba, São Paulo, Brazil., Caiaffa KS; Doctoral student, Department of Pediatric and Public Health, Araçatuba Dental School, São Paulo State University (UNESP), São Paulo, Brazil., Brandini DA; Assistant Professor, Department of Surgery and Integrated Clinic, Araçatuba Dental School, São Paulo State University (UNESP), São Paulo, Brazil., Narciso de Oliveira DT; Doctoral student, Department of Dermatologic, Infectious and Parasitic Diseases, Medical School of São José do Rio Preto (FAMERP), São Paulo, Brazil., Brizzotti NS; Biologist, Department of Dermatologic, Infectious and Parasitic Diseases, Medical School of São José do Rio Preto (FAMERP), São Paulo, Brazil., Gottardo de Almeida MT; Professor, Department of Dermatologic, Infectious and Parasitic Diseases, Medical School of São José do Rio Preto (FAMERP), São Paulo, Brazil. |
| المصدر: | The Journal of prosthetic dentistry [J Prosthet Dent] 2016 Jul; Vol. 116 (1), pp. 136-43. Date of Electronic Publication: 2016 Feb 26. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Mosby-Year Book Country of Publication: United States NLM ID: 0376364 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1097-6841 (Electronic) Linking ISSN: 00223913 NLM ISO Abbreviation: J Prosthet Dent Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: St. Louis Mo : Mosby-Year Book Original Publication: St. Louis, Mosby. |
| مواضيع طبية MeSH: | Anti-Infective Agents/*pharmacology , Biofilms/*drug effects , Dental Disinfectants/*pharmacology , Plant Extracts/*pharmacology , Prostheses and Implants/*microbiology, Candida albicans/drug effects ; Cymbopogon/chemistry ; Facial Bones ; Humans ; Hydrastis/chemistry ; Maxilla ; Silicones ; Staphylococcus aureus/drug effects |
| مستخلص: | Statement of Problem: Dentists often note problems with infection in patients with maxillofacial prostheses. Conventional disinfection protocols are not always effective and may alter the properties of the polymer used in the prosthesis. Thus, the search for improved disinfection methods is important. Purpose: The purpose of this in vitro study was to evaluate and compare the antimicrobial activity of conventional disinfectant solutions (water and neutral soap and 4% chlorhexidine) and plant extracts (Cymbopogon nardus and Hydrastis canadensis) on specimens of maxillofacial silicone contaminated with Candida albicans and Staphylococcus aureus biofilms. Material and Methods: Seventy-two silicone (MDX4-4210) specimens were fabricated (5×2 mm) and sterilized. Thirty-six were contaminated with C albicans (10(6) cells/mL) and 36 with S aureus (10(8) cells/mL) to evaluate the antimicrobial activity of the cleaning protocols. After incubation (37°C/72 hours), the specimens were divided into 5 groups: not disinfected (positive control), soaking in saline solution for 10 minutes, soaking in 4% chlorhexidine for 10 minutes, soaking in C nardus for 10 minutes, soaking in H canadensis for 10 minutes, and washing by hand with water and neutral soap for 30 seconds. The viability of cells was evaluated by XTT (2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide) assay and by scanning electron microscope analysis. The results were analyzed by ANOVA and the Tukey HSD test (α=.05). Results: All disinfection solutions provided a statistically significant reduction in biofilm viability compared with the control group for both microorganisms (P<.05). Washing with water and neutral soap was significantly more effective in reducing biofilm viability than immersion in the disinfection solutions, with persistence of viable microorganisms between 1.05% for C albicans and 0.62% for S aureus after this cleaning protocol. Photomicrographs revealed that 4% chlorhexidine altered the surface of the polymer. Conclusions: Within the limitations of this in vitro study, it was concluded that the cleaning protocols with different disinfectant solutions produced a significant reduction in the viability of C albicans and S aureus biofilms on the silicone polymer. Washing with water and neutral soap was the most effective protocol against both microorganisms. (Copyright © 2016 Editorial Council for the Journal of Prosthetic Dentistry. Published by Elsevier Inc. All rights reserved.) |
| المشرفين على المادة: | 0 (Anti-Infective Agents) 0 (Dental Disinfectants) 0 (Plant Extracts) 0 (Silicones) |
| تواريخ الأحداث: | Date Created: 20160229 Date Completed: 20171218 Latest Revision: 20171218 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1016/j.prosdent.2015.12.014 |
| PMID: | 26922209 |
| قاعدة البيانات: | MEDLINE |
Full Text via ClinicalKey 
Full Text Finder | تدمد: | 1097-6841 |
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| DOI: | 10.1016/j.prosdent.2015.12.014 |