دورية أكاديمية
Genetic Tagging During Human Mesoderm Differentiation Reveals Tripotent Lateral Plate Mesodermal Progenitors.
العنوان: | Genetic Tagging During Human Mesoderm Differentiation Reveals Tripotent Lateral Plate Mesodermal Progenitors. |
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المؤلفون: | Chin CJ; Department of Pathology & Laboratory Medicine, David Geffen School of Medicine (DGSOM)., Cooper AR; Molecular Biology Interdepartmental PhD Program, DGSOM University of California Los Angeles.; Department of Microbiology, Immunology and Molecular Genetics, DGSOM, DGSOM University of California Los Angeles., Lill GR; Department of Microbiology, Immunology and Molecular Genetics, DGSOM, DGSOM University of California Los Angeles., Evseenko D; Department of Orthopedic Surgery, Keck School of Medicine of University of Southern California (USC). All in, Los Angeles, CA, United States., Zhu Y; Department of Pathology & Laboratory Medicine, David Geffen School of Medicine (DGSOM)., He CB; Department of Pathology & Laboratory Medicine, David Geffen School of Medicine (DGSOM)., Casero D; Department of Pathology & Laboratory Medicine, David Geffen School of Medicine (DGSOM)., Pellegrini M; Department of Molecular, Cell and Development Biology, DGSOM University of California Los Angeles.; Molecular Biology Institute (MBI)., Kohn DB; Department of Microbiology, Immunology and Molecular Genetics, DGSOM, DGSOM University of California Los Angeles.; Molecular Biology Institute (MBI).; Department of Pediatrics, DGSOM University of California Los Angeles.; Broad Stem Cell Research Center (BSCRC), DGSOM University of California Los Angeles.; Jonsson Comprehensive Cancer Center (JCCC), DGSOM University of California Los Angeles., Crooks GM; Department of Pathology & Laboratory Medicine, David Geffen School of Medicine (DGSOM).; Department of Pediatrics, DGSOM University of California Los Angeles.; Broad Stem Cell Research Center (BSCRC), DGSOM University of California Los Angeles.; Department of Pathology & Laboratory Medicine, DGSOM University of California Los Angeles. |
المصدر: | Stem cells (Dayton, Ohio) [Stem Cells] 2016 May; Vol. 34 (5), pp. 1239-50. Date of Electronic Publication: 2016 Mar 28. |
نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
اللغة: | English |
بيانات الدورية: | Publisher: Oxford University Press Country of Publication: England NLM ID: 9304532 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1549-4918 (Electronic) Linking ISSN: 10665099 NLM ISO Abbreviation: Stem Cells Subsets: MEDLINE |
أسماء مطبوعة: | Publication: 2022- : Oxford : Oxford University Press Original Publication: Dayton, OH : AlphaMed Press, c1993- |
مواضيع طبية MeSH: | Cell Differentiation* , Genetic Techniques*, Mesoderm/*cytology , Multipotent Stem Cells/*cytology, Animals ; Antigens, CD/metabolism ; Cell Line ; Cell Lineage ; Cell Separation ; Clone Cells ; Flow Cytometry ; Humans ; Lentivirus/metabolism ; Mice |
مستخلص: | Although clonal studies of lineage potential have been extensively applied to organ specific stem and progenitor cells, much less is known about the clonal origins of lineages formed from the germ layers in early embryogenesis. We applied lentiviral tagging followed by vector integration site analysis (VISA) with high-throughput sequencing to investigate the ontogeny of the hematopoietic, endothelial and mesenchymal lineages as they emerge from human embryonic mesoderm. In contrast to studies that have used VISA to track differentiation of self-renewing stem cell clones that amplify significantly over time, we focused on a population of progenitor clones with limited self-renewal capability. Our analyses uncovered the critical influence of sampling on the interpretation of lentiviral tag sharing, particularly among complex populations with minimal clonal duplication. By applying a quantitative framework to estimate the degree of undersampling we revealed the existence of tripotent mesodermal progenitors derived from pluripotent stem cells, and the subsequent bifurcation of their differentiation into bipotent endothelial/hematopoietic or endothelial/mesenchymal progenitors. Stem Cells 2016;34:1239-1250. Competing Interests: of Potential Conflicts of Interest The authors indicate no potential conflicts of interest. (© 2016 AlphaMed Press.) |
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معلومات مُعتمدة: | K01 AR061415 United States AR NIAMS NIH HHS; P01 HL073104 United States HL NHLBI NIH HHS; T32 GM007185 United States GM NIGMS NIH HHS |
فهرسة مساهمة: | Keywords: Clonal tracking; Hematopoiesis; Lentiviral vectors; Lineage tracing; Mesoderm; Pluripotent stem cells; Vector integration site analysis |
المشرفين على المادة: | 0 (Antigens, CD) |
تواريخ الأحداث: | Date Created: 20160303 Date Completed: 20171204 Latest Revision: 20181113 |
رمز التحديث: | 20221213 |
مُعرف محوري في PubMed: | PMC5052131 |
DOI: | 10.1002/stem.2351 |
PMID: | 26934332 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1549-4918 |
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DOI: | 10.1002/stem.2351 |