دورية أكاديمية
أعرض في EDS

Collagen Fingerprinting: A New Screening Technique for Radiocarbon Dating Ancient Bone.

التفاصيل البيبلوغرافية
العنوان: Collagen Fingerprinting: A New Screening Technique for Radiocarbon Dating Ancient Bone.
المؤلفون: Harvey VL; School of Earth, Atmospheric and Environmental Sciences, The University of Manchester, Manchester, M13 9PL, United Kingdom., Egerton VM; School of Earth, Atmospheric and Environmental Sciences, The University of Manchester, Manchester, M13 9PL, United Kingdom.; Department of Geology and Environmental Geosciences, College of Charleston, 66 George Street, Charleston, South Carolina, 29424, United States of America., Chamberlain AT; Faculty of Life Sciences, The University of Manchester, Manchester M13 9PL, United Kingdom., Manning PL; School of Earth, Atmospheric and Environmental Sciences, The University of Manchester, Manchester, M13 9PL, United Kingdom.; Department of Geology and Environmental Geosciences, College of Charleston, 66 George Street, Charleston, South Carolina, 29424, United States of America., Buckley M; Faculty of Life Sciences, The University of Manchester, Manchester M13 9PL, United Kingdom.
المصدر: PloS one [PLoS One] 2016 Mar 03; Vol. 11 (3), pp. e0150650. Date of Electronic Publication: 2016 Mar 03 (Print Publication: 2016).
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
أسماء مطبوعة: Original Publication: San Francisco, CA : Public Library of Science
مواضيع طبية MeSH: Bone and Bones/*chemistry , Collagen/*chemistry , Mass Spectrometry/*methods , Radiometric Dating/*methods, Animals ; Archaeology/methods ; Biodiversity ; Calibration ; Carbon/chemistry ; Carbon Radioisotopes/analysis ; Fossils ; Humans ; Nitrogen/chemistry ; Paleontology ; Peptides/chemistry ; Rodentia ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ; West Indies
مستخلص: Collagen is the dominant organic component of bone and is intimately locked within the hydroxyapatite structure of this ubiquitous biomaterial that dominates archaeological and palaeontological assemblages. Radiocarbon analysis of extracted collagen is one of the most common approaches to dating bone from late Pleistocene or Holocene deposits, but dating is relatively expensive compared to other biochemical techniques. Numerous analytical methods have previously been investigated for the purpose of screening out samples that are unlikely to yield reliable dates including histological analysis, UV-stimulated fluorescence and, most commonly, the measurement of percentage nitrogen (%N) and ratio of carbon to nitrogen (C:N). Here we propose the use of collagen fingerprinting (also known as Zooarchaeology by Mass Spectrometry, or ZooMS, when applied to species identification) as an alternative screening method for radiocarbon dating, due to its ability to provide information on collagen presence and quality, alongside species identification. The method was tested on a series of sub-fossil bone specimens from cave systems on Cayman Brac (Cayman Islands), chosen due to the observable range in diagenetic alteration, and in particular, the extent of mineralisation. Six (14)C dates, of 18 initial attempts, were obtained from remains of extinct hutia, Capromys sp. (Rodentia; Capromyidae), recovered from five distinct caves on Cayman Brac, and ranging from 393 ± 25 to 1588 ± 26 radiocarbon years before present (yr BP). All of the bone samples that yielded radiocarbon dates generated excellent collagen fingerprints, and conversely those that gave poor fingerprints also failed dating. Additionally, two successfully fingerprinted bone samples were screened out from a set of 81. Both subsequently generated (14)C dates, demonstrating successful utilisation of ZooMS as an alternative screening mechanism to identify bone samples that are suitable for 1(4)C analysis.
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المشرفين على المادة: 0 (Carbon Radioisotopes)
0 (Peptides)
7440-44-0 (Carbon)
9007-34-5 (Collagen)
N762921K75 (Nitrogen)
تواريخ الأحداث: Date Created: 20160304 Date Completed: 20160802 Latest Revision: 20190219
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC4777535
DOI: 10.1371/journal.pone.0150650
PMID: 26938469
قاعدة البيانات: MEDLINE
الوصف
تدمد:1932-6203
DOI:10.1371/journal.pone.0150650