التفاصيل البيبلوغرافية
| العنوان: |
Labeling of Salmonella typhimurium with iodine-131 to study phagocytic function in rats. |
| المؤلفون: |
Sato MK, Rodrigues Júnior AJ, Camargo EE |
| المصدر: |
Revista do Instituto de Medicina Tropical de Sao Paulo [Rev Inst Med Trop Sao Paulo] 1989 May-Jun; Vol. 31 (3), pp. 135-8. |
| نوع المنشور: |
Journal Article |
| اللغة: |
English |
| بيانات الدورية: |
Publisher: Universidade de São Paulo, Instituto de Medicina Tropical de São Paulo Country of Publication: Brazil NLM ID: 7507484 Publication Model: Print Cited Medium: Print ISSN: 0036-4665 (Print) Linking ISSN: 00364665 NLM ISO Abbreviation: Rev Inst Med Trop Sao Paulo Subsets: MEDLINE |
| أسماء مطبوعة: |
Original Publication: Sao Paulo : Universidade de São Paulo, Instituto de Medicina Tropical de São Paulo |
| مواضيع طبية MeSH: |
Iodine Radioisotopes* , Phagocytosis* , Salmonella typhimurium*, Mononuclear Phagocyte System/*physiology, Animals ; Autoradiography ; Liver/diagnostic imaging ; Radiography ; Rats ; Rats, Inbred Strains ; Spleen/diagnostic imaging |
| مستخلص: |
The present study describes a method for labeling Salmonella typhymurium with iodine-131 to evaluate both the morphological and the functional characteristics of the reticulo-endothelial system. A suspension containing 2 x 10(9) bacteria per ml was labeled with carrier-free Na131I without reductor, with a labeling yield of 46.5 +/- 3% and 3.5 +/- 1.3% of free Iodine-131. The biodistribution of the labeled bacteria in rats was studied with a large-field-of-view scintillation camera equiped with a pinhole collimator. Whole body images were obtained 15 and 30 minutes after intravenous injection of the labeled microorganisms. Images showed accumulation of bacteria in the liver and both normal and transplanted spleens of the animals. Autoradiographs of liver and spleen demonstrated labeled bacteria within the cells of the reticulo-endothelial system. The method described is easy to perform, has a good labeling yield and allows the functional evaluation of the reticulo-monophagocytic system, including transplanted spleens. |
| المشرفين على المادة: |
0 (Iodine Radioisotopes) |
| تواريخ الأحداث: |
Date Created: 19890501 Date Completed: 19900226 Latest Revision: 20190606 |
| رمز التحديث: |
20221208 |
| DOI: |
10.1590/s0036-46651989000300001 |
| PMID: |
2694304 |
| قاعدة البيانات: |
MEDLINE |
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