دورية أكاديمية
أعرض في EDS

Heterotopic ovarian transplantation results in less apoptosis than orthotopic transplantation in a minipig model.

التفاصيل البيبلوغرافية
العنوان: Heterotopic ovarian transplantation results in less apoptosis than orthotopic transplantation in a minipig model.
المؤلفون: Damásio LC; Disciplina de Ginecologia, Departamento de Obstetrícia e Ginecologia, Hospital das Clínicas, Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil. liacruzcosta@yahoo.com.br.; Instituto de Ensino e Pesquisa do Hospital Israelita Albert Einstein, São Paulo, Brazil. liacruzcosta@yahoo.com.br., Soares-Júnior JM; Disciplina de Ginecologia, Departamento de Obstetrícia e Ginecologia, Hospital das Clínicas, Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil., Iavelberg J; Disciplina de Ginecologia, Departamento de Obstetrícia e Ginecologia, Hospital das Clínicas, Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil., Maciel GA; Disciplina de Ginecologia, Departamento de Obstetrícia e Ginecologia, Hospital das Clínicas, Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil., de Jesus Simões M; Disciplina de Ginecologia, Departamento de Obstetrícia e Ginecologia, Hospital das Clínicas, Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil., Dos Santos Simões R; Disciplina de Ginecologia, Departamento de Obstetrícia e Ginecologia, Hospital das Clínicas, Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil., da Motta EV; Disciplina de Ginecologia, Departamento de Obstetrícia e Ginecologia, Hospital das Clínicas, Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil.; Instituto de Ensino e Pesquisa do Hospital Israelita Albert Einstein, São Paulo, Brazil., Baracat MC; Disciplina de Ginecologia, Departamento de Obstetrícia e Ginecologia, Hospital das Clínicas, Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil., Baracat EC; Disciplina de Ginecologia, Departamento de Obstetrícia e Ginecologia, Hospital das Clínicas, Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil.; Instituto de Ensino e Pesquisa do Hospital Israelita Albert Einstein, São Paulo, Brazil.
المصدر: Journal of ovarian research [J Ovarian Res] 2016 Mar 15; Vol. 9, pp. 14. Date of Electronic Publication: 2016 Mar 15.
نوع المنشور: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: BioMed Central Country of Publication: England NLM ID: 101474849 Publication Model: Electronic Cited Medium: Internet ISSN: 1757-2215 (Electronic) Linking ISSN: 17572215 NLM ISO Abbreviation: J Ovarian Res Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [London] : BioMed Central, c2008-
مواضيع طبية MeSH: Apoptosis*, Ovary/*transplantation, Animals ; Apoptosis Regulatory Proteins/metabolism ; Autografts ; Female ; Ovary/cytology ; Swine ; Swine, Miniature
مستخلص: Background: Ovarian autotransplantation has shown increasing promise as a clinical method for the preservation of fertility and hormonal function. However, information regarding the success rate of this type of transplantation is limited. We hypothesized that results vary according to the site of the ovarian transplantation. To test this hypothesis, fresh or cryopreserved ovarian strips were autotransplanted to orthotopic or heterotopic sites. The strips were later collected, and the morphology and expression of selected markers of apoptosis were evaluated. We compared the Bax, Bcl-2 and cleaved caspase-3 staining levels and the morphometric aspects of autotransplanted fresh and cryopreserved ovarian strips placed at orthotopic and heterotopic sites in minipigs.
Methods: Forty female minipigs were allocated to the following five groups: group 1 (control), ovarian tissue removed during oophorectomy; group 2, transplantation of fresh ovarian strips to a heterotopic site; group 3, transplantation of fresh ovarian strips to an orthotopic site; group 4, transplantation of cryopreserved ovarian strips to a heterotopic site; and group 5, transplantation of ovarian trips to an orthotopic site. On day 7 after transplantation, ovarian strips were collected, and the morphology and expression of apoptosis markers were evaluated.
Results: In all groups, follicles across all stages of development were detected. The numbers of primordial, primary and secondary follicles were similar in all groups, but the numbers of antral follicles were lower in the cryopreserved groups in comparison with freshly derived ovarian tissue, with no significant differences observed between fresh and cryopreserved transplants. In all transplanted groups, Bcl-2 expression was lower and Bax expression was higher than in the control group. Furthermore, increased expression of apoptosis markers was detected in fresh intraperitoneal transplants. Lastly, the expression of cleaved caspase-3 was higher in the cryopreserved orthotopic group compared with the heterotopic group.
Conclusions: Orthotopic and heterotopic ovarian strip transplantations are feasible options using these techniques. Importantly, we found that heterotopic transplantation preserves ovarian follicle integrity to a greater degree (i.e., lower expression of apoptosis markers) than orthotopic transplantation, and cryopreservation does not exacerbate expression of apoptosis's markers. These findings have major clinical applications and enhance the discussion regarding the heterotopic transplantation of ovarian tissue.
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فهرسة مساهمة: Keywords: Apoptosis; Cleaved caspase 3; Follicle number; Ovarian transplantation; Pig
المشرفين على المادة: 0 (Apoptosis Regulatory Proteins)
تواريخ الأحداث: Date Created: 20160317 Date Completed: 20161011 Latest Revision: 20181113
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC4791910
DOI: 10.1186/s13048-016-0223-1
PMID: 26979065
قاعدة البيانات: MEDLINE
الوصف
تدمد:1757-2215
DOI:10.1186/s13048-016-0223-1