دورية أكاديمية
أعرض في EDS

Multiplex determination of serological signatures in the sera of colorectal cancer patients using hydrogel biochips.

التفاصيل البيبلوغرافية
العنوان: Multiplex determination of serological signatures in the sera of colorectal cancer patients using hydrogel biochips.
المؤلفون: Butvilovskaya VI; Engelhardt Institute of Molecular Biology (IMB RAS), Russian Academy of Sciences, Moscow, Russia., Popletaeva SB; Engelhardt Institute of Molecular Biology (IMB RAS), Russian Academy of Sciences, Moscow, Russia., Chechetkin VR; Engelhardt Institute of Molecular Biology (IMB RAS), Russian Academy of Sciences, Moscow, Russia., Zubtsova ZI; Moscow Institute of Physics and Technology (State University), Moscow, Russia., Tsybulskaya MV; Engelhardt Institute of Molecular Biology (IMB RAS), Russian Academy of Sciences, Moscow, Russia., Samokhina LO; V. M. Petrovsky Russian Center of Science and Surgery, Russian Academy of Medical Sciences, Moscow, Russia., Vinnitskii LI; V. M. Petrovsky Russian Center of Science and Surgery, Russian Academy of Medical Sciences, Moscow, Russia., Ragimov AA; V. M. Petrovsky Russian Center of Science and Surgery, Russian Academy of Medical Sciences, Moscow, Russia., Pozharitskaya EI; Moscow City Clinical Hospital No. 12, Russia., Grigor Eva GA; I.M. Sechenov First Moscow State Medical University, Moscow, Russia., Meshalkina NY; I.M. Sechenov First Moscow State Medical University, Moscow, Russia., Golysheva SV; I.M. Sechenov First Moscow State Medical University, Moscow, Russia., Shilova NV; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia., Bovin NV; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia., Zasedatelev AS; Engelhardt Institute of Molecular Biology (IMB RAS), Russian Academy of Sciences, Moscow, Russia., Rubina AY; Engelhardt Institute of Molecular Biology (IMB RAS), Russian Academy of Sciences, Moscow, Russia.
المصدر: Cancer medicine [Cancer Med] 2016 Jul; Vol. 5 (7), pp. 1361-72. Date of Electronic Publication: 2016 Mar 19.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: John Wiley & Sons Ltd Country of Publication: United States NLM ID: 101595310 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2045-7634 (Electronic) Linking ISSN: 20457634 NLM ISO Abbreviation: Cancer Med Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [Malden, MA] : John Wiley & Sons Ltd., c2012-
مواضيع طبية MeSH: Biomarkers, Tumor* , Protein Array Analysis*/methods, Colorectal Neoplasms/*blood , Colorectal Neoplasms/*diagnosis, Aged ; Aged, 80 and over ; Antibodies/blood ; Antibodies/immunology ; Antigens, Neoplasm/immunology ; Case-Control Studies ; Female ; Humans ; Hydrogel, Polyethylene Glycol Dimethacrylate ; Male ; Middle Aged ; Neoplasm Staging ; Polysaccharides/immunology ; ROC Curve ; Sensitivity and Specificity
مستخلص: Colorectal cancer (CRC) is the third most common malignancy in industrialized countries. Despite the advances in diagnostics and development of new drugs, the 5-year survival remains only 60-65%. Our approach to early diagnostics of CRC is based on the determination of serological signatures with an array of hemispherical hydrogel cells containing immobilized proteins and oligosaccharides (glycochip). The compounds immobilized on the glycochip include tumor-associated glycans (SiaTn, Tn, TF, Le(C) , Le(Y) , SiaLe(A) , and Manβ1-4GlcNAcβ) and antibodies against human immunoglobulins IgG, IgA, and IgM. The glycochip detects antibodies against tumor-associated glycans in patients' sera. The simultaneous measurement of the levels of immunoglobulins enhances the diagnostic impact of the signatures. In this work, we found previously unreported increase in antibodies against oligosaccharide Manβ1-4GlcNAcβ in patients with CRC. In parallel with these experiments, we determined the levels of oncomarkers carcinoembryonic antigen (CEA), cancer antigen (CA) 19-9, CA 125, CA 15-3, human chorionic gonadotropin (HCG), and alpha-fetoprotein (AFP) using another gel-based biochip with immobilized antibodies (oncochip) developed earlier in our laboratory. In total, 69 samples from healthy donors, 33 from patients with colorectal carcinoma, and 27 from patients with inflammatory bowel diseases were studied. The use of combined signatures of antiglycan antibodies and oncomarkers provides much better predictive value than the conventional measurement of oncomarkers CEA and CA 19-9. Positive predictive value of CRC diagnoses using together glycochip and oncochip reached 95% with the sensitivity and specificity 88% and 98%, respectively. Thus, the combination of antibody profiling with detection of conventional oncomarkers proved to be a promising tool in diagnostics of CRC.
(© 2016 The Authors. Cancer Medicine published by John Wiley & Sons Ltd.)
References: Eur J Surg Oncol. 1992 Feb;18(1):31-6. (PMID: 1737590)
Fam Cancer. 2013 Sep;12(3):459-72. (PMID: 23700069)
J Mol Med (Berl). 1997 Aug;75(8):594-602. (PMID: 9297627)
Cancer Invest. 2005;23(4):338-51. (PMID: 16100946)
Hepatogastroenterology. 2008 May-Jun;55(84):991-7. (PMID: 18705314)
Am J Gastroenterol. 2009 Mar;104(3):739-50. (PMID: 19240699)
Ann Oncol. 2007 May;18(5):868-73. (PMID: 17347129)
Anticancer Res. 2000 Nov-Dec;20(6D):5195-8. (PMID: 11326694)
Thorax. 2008 Mar;63(3):228-33. (PMID: 17932110)
Tumour Biol. 2004 Sep-Dec;25(5-6):228-34. (PMID: 15627885)
Anal Chem. 2006 Sep 15;78(18):6476-83. (PMID: 16970323)
Bioorg Khim. 2013 Nov-Dec;39(6):693-704. (PMID: 25696931)
Aust N Z J Surg. 1981 Oct;51(5):440-2. (PMID: 6947782)
Nat Rev Drug Discov. 2005 Jun;4(6):477-88. (PMID: 15931257)
Metabolites. 2012 Nov 14;2(4):913-39. (PMID: 24957768)
J Clin Oncol. 1996 Oct;14(10):2843-77. (PMID: 8874347)
Int J Cancer. 2012 Jan 1;130(1):138-46. (PMID: 21351089)
Clin Chem. 1983 Mar;29(3):549-52. (PMID: 6825270)
Int J Bioinform Res Appl. 2011;7(4):402-26. (PMID: 22112531)
Ai Zheng. 2007 Nov;26(11):1221-6. (PMID: 17991322)
Cancer Metastasis Rev. 2004 Jan-Jun;23(1-2):77-99. (PMID: 15000151)
Biotechniques. 2003 May;34(5):1008-14, 1016-20, 1022. (PMID: 12765028)
Nat Rev Cancer. 2004 Jan;4(1):45-60. (PMID: 14681689)
Glycobiology. 2009 Apr;19(4):382-98. (PMID: 19122213)
Neoplasia. 2002 Mar-Apr;4(2):151-63. (PMID: 11896570)
Anal Biochem. 2005 Dec 1;347(1):94-105. (PMID: 16236238)
Br J Cancer. 2014 Oct 14;111(8):1634-45. (PMID: 25167227)
Lancet. 2015 Mar 14;385(9972):977-1010. (PMID: 25467588)
Biochim Biophys Acta. 2006 Apr;1760(4):603-9. (PMID: 16466673)
Cancer Med. 2016 Jul;5(7):1361-72. (PMID: 26992329)
Gastroenterology. 2010 Jun;138(6):2127-39. (PMID: 20420950)
J Natl Cancer Inst. 1975 Jan;54(1):53-56. (PMID: 1113312)
Biomark Insights. 2008 Jan 21;3:1-18. (PMID: 19578489)
Bull World Health Organ. 1975;52(2):179-85. (PMID: 1083305)
Eur J Cancer. 2007 Jun;43(9):1348-60. (PMID: 17512720)
Ont Health Technol Assess Ser. 2009;9(10):1-40. (PMID: 23074514)
N Engl J Med. 2014 Apr 3;370(14):1287-97. (PMID: 24645800)
Proteomics. 2008 Feb;8(4):817-31. (PMID: 18214844)
Dis Colon Rectum. 2004 Feb;47(2):227-32. (PMID: 15043294)
Int J Cancer. 2012 Jul 1;131(1):117-28. (PMID: 21823122)
Lupus. 2006;15(7):442-50. (PMID: 16898180)
Nat Rev Cancer. 2005 Jul;5(7):526-42. (PMID: 16069816)
CA Cancer J Clin. 2008 May-Jun;58(3):130-60. (PMID: 18322143)
Science. 1981 Apr 3;212(4490):53-5. (PMID: 6163212)
Cancer Res. 2005 Jul 1;65(13):5935-44. (PMID: 15994972)
فهرسة مساهمة: Keywords: Colorectal cancer; hydrogel biochips; tumor antibodies; tumor markers; tumor-associated glycans
المشرفين على المادة: 0 (Antibodies)
0 (Antigens, Neoplasm)
0 (Biomarkers, Tumor)
0 (Polysaccharides)
25852-47-5 (Hydrogel, Polyethylene Glycol Dimethacrylate)
تواريخ الأحداث: Date Created: 20160320 Date Completed: 20171116 Latest Revision: 20231111
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC4944861
DOI: 10.1002/cam4.692
PMID: 26992329
قاعدة البيانات: MEDLINE
الوصف
تدمد:2045-7634
DOI:10.1002/cam4.692