دورية أكاديمية
Multiplex determination of serological signatures in the sera of colorectal cancer patients using hydrogel biochips.
العنوان: | Multiplex determination of serological signatures in the sera of colorectal cancer patients using hydrogel biochips. |
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المؤلفون: | Butvilovskaya VI; Engelhardt Institute of Molecular Biology (IMB RAS), Russian Academy of Sciences, Moscow, Russia., Popletaeva SB; Engelhardt Institute of Molecular Biology (IMB RAS), Russian Academy of Sciences, Moscow, Russia., Chechetkin VR; Engelhardt Institute of Molecular Biology (IMB RAS), Russian Academy of Sciences, Moscow, Russia., Zubtsova ZI; Moscow Institute of Physics and Technology (State University), Moscow, Russia., Tsybulskaya MV; Engelhardt Institute of Molecular Biology (IMB RAS), Russian Academy of Sciences, Moscow, Russia., Samokhina LO; V. M. Petrovsky Russian Center of Science and Surgery, Russian Academy of Medical Sciences, Moscow, Russia., Vinnitskii LI; V. M. Petrovsky Russian Center of Science and Surgery, Russian Academy of Medical Sciences, Moscow, Russia., Ragimov AA; V. M. Petrovsky Russian Center of Science and Surgery, Russian Academy of Medical Sciences, Moscow, Russia., Pozharitskaya EI; Moscow City Clinical Hospital No. 12, Russia., Grigor Eva GA; I.M. Sechenov First Moscow State Medical University, Moscow, Russia., Meshalkina NY; I.M. Sechenov First Moscow State Medical University, Moscow, Russia., Golysheva SV; I.M. Sechenov First Moscow State Medical University, Moscow, Russia., Shilova NV; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia., Bovin NV; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia., Zasedatelev AS; Engelhardt Institute of Molecular Biology (IMB RAS), Russian Academy of Sciences, Moscow, Russia., Rubina AY; Engelhardt Institute of Molecular Biology (IMB RAS), Russian Academy of Sciences, Moscow, Russia. |
المصدر: | Cancer medicine [Cancer Med] 2016 Jul; Vol. 5 (7), pp. 1361-72. Date of Electronic Publication: 2016 Mar 19. |
نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
اللغة: | English |
بيانات الدورية: | Publisher: John Wiley & Sons Ltd Country of Publication: United States NLM ID: 101595310 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2045-7634 (Electronic) Linking ISSN: 20457634 NLM ISO Abbreviation: Cancer Med Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: [Malden, MA] : John Wiley & Sons Ltd., c2012- |
مواضيع طبية MeSH: | Biomarkers, Tumor* , Protein Array Analysis*/methods, Colorectal Neoplasms/*blood , Colorectal Neoplasms/*diagnosis, Aged ; Aged, 80 and over ; Antibodies/blood ; Antibodies/immunology ; Antigens, Neoplasm/immunology ; Case-Control Studies ; Female ; Humans ; Hydrogel, Polyethylene Glycol Dimethacrylate ; Male ; Middle Aged ; Neoplasm Staging ; Polysaccharides/immunology ; ROC Curve ; Sensitivity and Specificity |
مستخلص: | Colorectal cancer (CRC) is the third most common malignancy in industrialized countries. Despite the advances in diagnostics and development of new drugs, the 5-year survival remains only 60-65%. Our approach to early diagnostics of CRC is based on the determination of serological signatures with an array of hemispherical hydrogel cells containing immobilized proteins and oligosaccharides (glycochip). The compounds immobilized on the glycochip include tumor-associated glycans (SiaTn, Tn, TF, Le(C) , Le(Y) , SiaLe(A) , and Manβ1-4GlcNAcβ) and antibodies against human immunoglobulins IgG, IgA, and IgM. The glycochip detects antibodies against tumor-associated glycans in patients' sera. The simultaneous measurement of the levels of immunoglobulins enhances the diagnostic impact of the signatures. In this work, we found previously unreported increase in antibodies against oligosaccharide Manβ1-4GlcNAcβ in patients with CRC. In parallel with these experiments, we determined the levels of oncomarkers carcinoembryonic antigen (CEA), cancer antigen (CA) 19-9, CA 125, CA 15-3, human chorionic gonadotropin (HCG), and alpha-fetoprotein (AFP) using another gel-based biochip with immobilized antibodies (oncochip) developed earlier in our laboratory. In total, 69 samples from healthy donors, 33 from patients with colorectal carcinoma, and 27 from patients with inflammatory bowel diseases were studied. The use of combined signatures of antiglycan antibodies and oncomarkers provides much better predictive value than the conventional measurement of oncomarkers CEA and CA 19-9. Positive predictive value of CRC diagnoses using together glycochip and oncochip reached 95% with the sensitivity and specificity 88% and 98%, respectively. Thus, the combination of antibody profiling with detection of conventional oncomarkers proved to be a promising tool in diagnostics of CRC. (© 2016 The Authors. Cancer Medicine published by John Wiley & Sons Ltd.) |
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فهرسة مساهمة: | Keywords: Colorectal cancer; hydrogel biochips; tumor antibodies; tumor markers; tumor-associated glycans |
المشرفين على المادة: | 0 (Antibodies) 0 (Antigens, Neoplasm) 0 (Biomarkers, Tumor) 0 (Polysaccharides) 25852-47-5 (Hydrogel, Polyethylene Glycol Dimethacrylate) |
تواريخ الأحداث: | Date Created: 20160320 Date Completed: 20171116 Latest Revision: 20231111 |
رمز التحديث: | 20231215 |
مُعرف محوري في PubMed: | PMC4944861 |
DOI: | 10.1002/cam4.692 |
PMID: | 26992329 |
قاعدة البيانات: | MEDLINE |
تدمد: | 2045-7634 |
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DOI: | 10.1002/cam4.692 |