دورية أكاديمية
Implication of Rho-kinase and soluble guanylyl cyclase enzymes in prostate smooth muscle dysfunction in middle-aged rats.
| العنوان: | Implication of Rho-kinase and soluble guanylyl cyclase enzymes in prostate smooth muscle dysfunction in middle-aged rats. |
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| المؤلفون: | Calmasini FB; Faculty of Medical Sciences, Department of Pharmacology, University of Campinas (UNICAMP), Campinas, São Paulo, Brazil., Silva FH; Faculty of Medical Sciences, Department of Pharmacology, University of Campinas (UNICAMP), Campinas, São Paulo, Brazil., Alexandre EC; Faculty of Medical Sciences, Department of Pharmacology, University of Campinas (UNICAMP), Campinas, São Paulo, Brazil., Rodrigues RL; Faculty of Medical Sciences, Department of Pharmacology, University of Campinas (UNICAMP), Campinas, São Paulo, Brazil., Barbosa AP; Faculty of Medical Sciences, Department of Pharmacology, University of Campinas (UNICAMP), Campinas, São Paulo, Brazil., Ferrucci DL; Department of Structural and Functional Biology, University of Campinas-UNICAMP, Campinas, São Paulo, Brazil., Carvalho HF; Department of Structural and Functional Biology, University of Campinas-UNICAMP, Campinas, São Paulo, Brazil., Anhê GF; Department of Structural and Functional Biology, University of Campinas-UNICAMP, Campinas, São Paulo, Brazil., Pupo AS; Department of Pharmacology, Institute of Biosciences, University of São Paulo State (UNESP), Botucatu, São Paulo, Brazil., Antunes E; Faculty of Medical Sciences, Department of Pharmacology, University of Campinas (UNICAMP), Campinas, São Paulo, Brazil. |
| المصدر: | Neurourology and urodynamics [Neurourol Urodyn] 2017 Mar; Vol. 36 (3), pp. 589-596. Date of Electronic Publication: 2016 Mar 21. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Alan R. Liss Country of Publication: United States NLM ID: 8303326 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1520-6777 (Electronic) Linking ISSN: 07332467 NLM ISO Abbreviation: Neurourol Urodyn Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: New York : Alan R. Liss, c1982- |
| مواضيع طبية MeSH: | Muscle Contraction/*physiology , Muscle, Smooth/*metabolism , Prostate/*metabolism , Soluble Guanylyl Cyclase/*metabolism , rho-Associated Kinases/*metabolism, Animals ; Electric Stimulation ; Male ; Muscle, Smooth/physiopathology ; Norepinephrine/metabolism ; Prostate/physiopathology ; Rats ; Rats, Wistar ; Signal Transduction/physiology |
| مستخلص: | Aims: Aging is highly associated with benign prostate hyperplasia (BPH). We investigated here the alterations of the contractile and relaxant machinery in prostates of middle-aged rats, focusing on the Rho-kinase, nitric oxide (NO)-soluble guanylyl cyclase (sGC), α1- and β-adrenoceptor pathways. Methods: Male Wistar young (3.5-month old) and middle-aged rats (10-month old) were used. Quantitative image analysis of prostates and functional assays evaluating the prostate contractions and relaxations were employed. Measurement of [ 3 H]-noradrenaline efflux, western blotting for α1 and β1 sGC subunits, and cyclic nucleotide levels were carried out. Results: Prostates of middle-aged rats showed significant increases in lumen and smooth muscle cells, but no alterations in the relative prostate weight were observed. In vivo, noradrenaline (10 -7 -10 -4 g/kg) produced greater prostatic contractions in middle-aged compared with control rats. Likewise, the in vitro contractions to phenylephrine (1 nM-100 μM) and α,β-methylene ATP (1-10 μM) were greater in middle-aged rats. Electrical-field stimulation (EFS, 1-32 Hz) promoted higher [ 3 H]-noradrenaline efflux and prostate contractions in middle-aged rats. Reduced expressions of α1 and β1 sGC subunits and diminished NO-mediated prostate relaxations in middle-age were observed. Isoproterenol-induced relaxations and cAMP levels were reduced in prostates of middle-aged rats. The Rho-kinase inhibitor fasudil (50 mg/kg, 2 weeks) normalized the prostate hypercontractility in middle-age rats. Conclusions: Prostate hypercontractility in middle-aging is associated with increased release of noradrenaline and Rho-kinase pathway, as well as with impairments of NO-sGC and β-adrenoceptor pathways. Middle-aged rats are suitable to explore the enhanced prostatic tone in the absence of prostate overgrowth. Neurourol. Urodynam. 36:589-596, 2017. © 2016 Wiley Periodicals, Inc. (© 2016 Wiley Periodicals, Inc.) |
| فهرسة مساهمة: | Keywords: aging; cyclic AMP; protein kinase C; stereology; β-adrenoceptors |
| المشرفين على المادة: | EC 2.7.11.1 (rho-Associated Kinases) EC 4.6.1.2 (Soluble Guanylyl Cyclase) X4W3ENH1CV (Norepinephrine) |
| تواريخ الأحداث: | Date Created: 20160322 Date Completed: 20171221 Latest Revision: 20181202 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1002/nau.22990 |
| PMID: | 26999618 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1520-6777 |
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| DOI: | 10.1002/nau.22990 |