دورية أكاديمية
[THE HIGHLY EFFECTIVE DETECTION OF DNA RICKETTSIA USING TECHNIQUE OF POLYMERASE CHAIN REACTION IN REAL-TIME].
| العنوان: | [THE HIGHLY EFFECTIVE DETECTION OF DNA RICKETTSIA USING TECHNIQUE OF POLYMERASE CHAIN REACTION IN REAL-TIME]. |
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| المؤلفون: | Kartashov MY, Mikryukova TP, Ternovoi VA, Moskvitina NS, Loktev VB |
| المصدر: | Klinicheskaia laboratornaia diagnostika [Klin Lab Diagn] 2015 Dec; Vol. 60 (12), pp. 39-43. |
| نوع المنشور: | English Abstract; Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | Russian |
| بيانات الدورية: | Publisher: Meditsina Country of Publication: Russia (Federation) NLM ID: 9432021 Publication Model: Print Cited Medium: Print ISSN: 0869-2084 (Print) Linking ISSN: 08692084 NLM ISO Abbreviation: Klin Lab Diagn Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Moskva : "Meditsina", 1992- |
| مواضيع طبية MeSH: | Bacterial Proteins/*genetics , Citrate (si)-Synthase/*genetics , DNA, Bacterial/*genetics , Ixodes/*microbiology , Real-Time Polymerase Chain Reaction/*methods , Rickettsia/*genetics, Animals ; DNA Primers/chemical synthesis ; DNA Primers/chemistry ; DNA Probes/chemical synthesis ; DNA Probes/chemistry ; Gene Expression ; Ixodes/chemistry ; Rickettsia/classification ; Rickettsia/isolation & purification ; Russia ; Sensitivity and Specificity |
| مستخلص: | The article considers development of highly effective technique of detection of genetic material of ricketsia based on polymerase chain reaction in real-time using original primers to the most conservative sites of gene of citrate synthase (gItA). The analytical sensitivity of the developed polymerase chain reaction in real-time test permits to detect from 80 genome equivalents in analyzed sample during three hours. The high specificity of test-system is substantiated by detection of nucleotide sequences of amplificated fragments of gene gltA. The approbation ofthe polymerase chain reaction in real-time test is carried out on collection of 310 ticks of species I. persulcatus, I. pavlovskyi, D. reticulatus. It is demonstrated that the developed alternate ofprimers and probe permits with high degree of sensitivity and specifcity to detect DNA of different species of ricketsia widespread on territory of Russia (R. sibirica, R. raoultii, R. helvetica, R. tarasevichiae). The proposed polymerase chain reaction in real-time test can be appliedfor isolation of fragment of gene gltA with purpose for detecting nucleotide sequence and subsequent genetic typing of ricketsia. The application ofthe proposed technique can facilitate task of monitoring hot spots of ricketsiosis. |
| المشرفين على المادة: | 0 (Bacterial Proteins) 0 (DNA Primers) 0 (DNA Probes) 0 (DNA, Bacterial) EC 2.3.3.1 (Citrate (si)-Synthase) |
| تواريخ الأحداث: | Date Created: 20160402 Date Completed: 20160419 Latest Revision: 20190816 |
| رمز التحديث: | 20221213 |
| PMID: | 27032252 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0869-2084 |
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