دورية أكاديمية
أعرض في EDS

Trypanosoma cruzi Needs a Signal Provided by Reactive Oxygen Species to Infect Macrophages.

التفاصيل البيبلوغرافية
العنوان: Trypanosoma cruzi Needs a Signal Provided by Reactive Oxygen Species to Infect Macrophages.
المؤلفون: Goes GR; Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil., Rocha PS; Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil., Diniz AR; Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil., Aguiar PH; Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil., Machado CR; Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil., Vieira LQ; Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
المصدر: PLoS neglected tropical diseases [PLoS Negl Trop Dis] 2016 Apr 01; Vol. 10 (4), pp. e0004555. Date of Electronic Publication: 2016 Apr 01 (Print Publication: 2016).
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101291488 Publication Model: eCollection Cited Medium: Internet ISSN: 1935-2735 (Electronic) Linking ISSN: 19352727 NLM ISO Abbreviation: PLoS Negl Trop Dis Subsets: MEDLINE
أسماء مطبوعة: Original Publication: San Francisco, CA : Public Library of Science
مواضيع طبية MeSH: Macrophages, Peritoneal/*parasitology , Reactive Oxygen Species/*metabolism , Trypanosoma cruzi/*physiology, Animals ; Cells, Cultured ; Chagas Disease/parasitology ; Deoxyguanine Nucleotides/metabolism ; Disease Models, Animal ; Host-Parasite Interactions ; Hydrogen Peroxide/pharmacology ; Macrophages, Peritoneal/drug effects ; Membrane Glycoproteins/genetics ; Membrane Glycoproteins/metabolism ; Mice ; Mice, Inbred C57BL ; Mice, Knockout ; NADPH Oxidase 2 ; NADPH Oxidases/genetics ; NADPH Oxidases/metabolism ; Oxidative Stress ; Rhodamines/metabolism ; Trypanosoma cruzi/drug effects ; Trypanosoma cruzi/genetics ; Trypanosoma cruzi/growth & development
مستخلص: Background: During Trypanosoma cruzi infection, macrophages produce reactive oxygen species (ROS) in a process called respiratory burst. Several works have aimed to elucidate the role of ROS during T. cruzi infection and the results obtained are sometimes contradictory. T. cruzi has a highly efficiently regulated antioxidant machinery to deal with the oxidative burst, but the parasite macromolecules, particularly DNA, may still suffer oxidative damage. Guanine (G) is the most vulnerable base and its oxidation results in formation of 8-oxoG, a cellular marker of oxidative stress.
Methodology/principal Findings: In order to investigate the contribution of ROS in T. cruzi survival and infection, we utilized mice deficient in the gp91phox (Phox KO) subunit of NADPH oxidase and parasites that overexpress the enzyme EcMutT (from Escherichia coli) or TcMTH (from T. cruzi), which is responsible for removing 8-oxo-dGTP from the nucleotide pool. The modified parasites presented enhanced replication inside murine inflammatory macrophages from C57BL/6 WT mice when compared with control parasites. Interestingly, when Phox KO macrophages were infected with these parasites, we observed a decreased number of all parasites when compared with macrophages from C57BL/6 WT. Scavengers for ROS also decreased parasite growth in WT macrophages. In addition, treatment of macrophages or parasites with hydrogen peroxide increased parasite replication in Phox KO mice and in vivo.
Conclusions: Our results indicate a paradoxical role for ROS since modified parasites multiply better inside macrophages, but proliferation is significantly reduced when ROS is removed from the host cell. Our findings suggest that ROS can work like a signaling molecule, contributing to T. cruzi growth inside the cells.
References: Annu Rev Genet. 2004;38:445-76. (PMID: 15568983)
Anal Biochem. 2000 Mar 15;279(2):241-2. (PMID: 10706793)
J Bacteriol. 1992 Oct;174(20):6321-5. (PMID: 1328155)
Biochemistry. 1995 Nov 21;34(46):14997-5005. (PMID: 7578113)
Free Radic Biol Med. 2010 Dec 1;49(11):1666-73. (PMID: 20807564)
Science. 1993 Mar 19;259(5102):1739-42. (PMID: 8456300)
Proc Natl Acad Sci U S A. 2002 Oct 15;99(21):13453-8. (PMID: 12351682)
J Biol Chem. 2011 Feb 25;286(8):6627-40. (PMID: 21098483)
Nat Rev Mol Cell Biol. 2007 Oct;8(10):813-24. (PMID: 17848967)
J Clin Invest. 2012 Jul;122(7):2531-42. (PMID: 22728935)
Rev Inst Med Trop Sao Paulo. 1962 Nov-Dec;4:389-96. (PMID: 14015230)
Biochem J. 2005 Mar 15;386(Pt 3):401-16. (PMID: 15588255)
Chem Res Toxicol. 2006 Apr;19(4):491-505. (PMID: 16608160)
Parasitol Res. 2008 Mar;102(4):801-3. (PMID: 18094999)
J Biol Chem. 2004 Aug 13;279(33):34175-82. (PMID: 15155760)
Arch Insect Biochem Physiol. 2001 Oct;48(2):63-71. (PMID: 11568965)
PLoS Negl Trop Dis. 2012;6(2):e1492. (PMID: 22348160)
Am J Trop Med Hyg. 1997 Mar;56(3):329-34. (PMID: 9129538)
Nat Chem Biol. 2015 Jan;11(1):64-70. (PMID: 25402766)
Eukaryot Cell. 2013 Jan;12(1):91-100. (PMID: 23143683)
Nature. 2004 Sep 9;431(7005):217-21. (PMID: 15322558)
Anal Biochem. 1998 Jan 1;255(1):20-31. (PMID: 9448838)
Cell Biol Int. 2003;27(10):853-61. (PMID: 14499666)
Nature. 2014 Apr 10;508(7495):215-21. (PMID: 24695224)
Adv Enzymol Relat Areas Mol Biol. 1999;73:183-207. (PMID: 10218109)
Cell Signal. 2010 Feb;22(2):234-46. (PMID: 19800403)
Biochem J. 2002 Jun 15;364(Pt 3):787-94. (PMID: 12049643)
Science. 2005 Jul 15;309(5733):409-15. (PMID: 16020725)
Science. 2005 Jul 15;309(5733):473-6. (PMID: 16020736)
Antioxid Redox Signal. 2009 Dec;11(12):2985-3011. (PMID: 19505186)
Infect Immun. 1983 Sep;41(3):1322-31. (PMID: 6350185)
Int J Cell Biol. 2010;2010:null. (PMID: 20811486)
PLoS Pathog. 2014 Dec;10(12):e1004516. (PMID: 25474113)
J Biol Chem. 1992 Jan 5;267(1):166-72. (PMID: 1730583)
J Biol Chem. 2000 Mar 17;275(11):8220-5. (PMID: 10713147)
Biochem Pharmacol. 2005 Sep 15;70(6):811-23. (PMID: 15899473)
Int J Parasitol. 2009 Nov;39(13):1455-64. (PMID: 19505468)
Antioxid Redox Signal. 2008 Aug;10(8):1343-74. (PMID: 18522489)
Infect Immun. 1994 Nov;62(11):5133-41. (PMID: 7927797)
Science. 2006 Jun 30;312(5782):1882-3. (PMID: 16809515)
Mol Cell. 2007 Apr 13;26(1):1-14. (PMID: 17434122)
Proc Natl Acad Sci U S A. 1982 Apr;79(8):2584-8. (PMID: 7045866)
Anal Biochem. 1982 Oct;126(1):131-8. (PMID: 7181105)
J Immunol. 1974 May;112(5):1839-44. (PMID: 4361979)
Annu Rev Microbiol. 1995;49:175-200. (PMID: 8561458)
Antioxid Redox Signal. 2014 Feb 20;20(6):1000-37. (PMID: 23992156)
Biochim Biophys Acta. 2008 Nov;1780(11):1236-48. (PMID: 18395526)
Curr Opin Microbiol. 2009 Aug;12(4):415-21. (PMID: 19616990)
Antioxid Redox Signal. 2013 Sep 1;19(7):723-34. (PMID: 22458250)
J Clin Invest. 2012 Jul;122(7):2352-4. (PMID: 22728929)
J Reticuloendothel Soc. 1982 Jun;31(6):479-87. (PMID: 6288939)
J Biol Chem. 2002 May 10;277(19):17062-71. (PMID: 11842085)
J Bioenerg Biomembr. 2011 Aug;43(4):419-24. (PMID: 21732175)
Nature. 2006 Apr 13;440(7086):944-8. (PMID: 16612386)
J Clin Invest. 1984 Mar;73(3):599-601. (PMID: 6323522)
Immunol Lett. 2000 Feb 1;71(2):79-83. (PMID: 10714433)
J Pathol. 2011 Dec;225(4):583-96. (PMID: 21952987)
Arch Biochem Biophys. 2004 Dec 15;432(2):222-32. (PMID: 15542061)
Biochem J. 2000 Dec 15;352 Pt 3:755-61. (PMID: 11104683)
Physiol Rev. 2002 Jan;82(1):47-95. (PMID: 11773609)
Biochem J. 2008 Mar 1;410(2):359-68. (PMID: 17973627)
J Exp Med. 2013 Feb 11;210(2):401-16. (PMID: 23382545)
J Mass Spectrom. 2007 Nov;42(11):1422-32. (PMID: 17960573)
Mem Inst Oswaldo Cruz. 2008 May;103(3):271-6. (PMID: 18592100)
PLoS Pathog. 2011;7(2):e1001271. (PMID: 21304882)
Parasitol Res. 2004 Jan;92(2):113-20. (PMID: 14634799)
Mol Biochem Parasitol. 2004 Jan;133(1):37-43. (PMID: 14668010)
Eur J Cancer. 1996 Jan;32A(1):30-8. (PMID: 8695238)
Immunol Lett. 1992 Jun;33(1):35-40. (PMID: 1330900)
Biofactors. 2014 Mar-Apr;40(2):215-25. (PMID: 24281946)
Nat Genet. 1995 Feb;9(2):202-9. (PMID: 7719350)
Sci Signal. 2009;2(90):re7. (PMID: 19797270)
Infect Immun. 2013 Sep;81(9):3198-209. (PMID: 23774601)
Free Radic Biol Med. 2010 Jun 15;48(12):1636-43. (PMID: 20304045)
Biochemistry. 1986 Jun 17;25(12):3519-26. (PMID: 3718941)
Mol Aspects Med. 2009 Feb-Apr;30(1-2):99-110. (PMID: 18926849)
PLoS One. 2011;6(10):e25935. (PMID: 22022475)
Curr Cardiol Rep. 2000 May;2(3):206-11. (PMID: 10980894)
PLoS Negl Trop Dis. 2013;7(6):e2279. (PMID: 23785540)
J Immunol. 1990 Mar 15;144(6):2384-8. (PMID: 2155965)
J Exp Med. 1979 May 1;149(5):1056-68. (PMID: 376774)
المشرفين على المادة: 0 (Deoxyguanine Nucleotides)
0 (Membrane Glycoproteins)
0 (Reactive Oxygen Species)
0 (Rhodamines)
109244-58-8 (dihydrorhodamine 123)
139307-94-1 (8-oxodeoxyguanosine triphosphate)
BBX060AN9V (Hydrogen Peroxide)
EC 1.6.3.- (Cybb protein, mouse)
EC 1.6.3.- (NADPH Oxidase 2)
EC 1.6.3.- (NADPH Oxidases)
تواريخ الأحداث: Date Created: 20160402 Date Completed: 20160811 Latest Revision: 20190202
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC4818108
DOI: 10.1371/journal.pntd.0004555
PMID: 27035573
قاعدة البيانات: MEDLINE
الوصف
تدمد:1935-2735
DOI:10.1371/journal.pntd.0004555