دورية أكاديمية
A Simplified Method for the Aspiration of Bone Marrow from Patients Undergoing Hip and Knee Joint Replacement for Isolating Mesenchymal Stem Cells and In Vitro Chondrogenesis.
العنوان: | A Simplified Method for the Aspiration of Bone Marrow from Patients Undergoing Hip and Knee Joint Replacement for Isolating Mesenchymal Stem Cells and In Vitro Chondrogenesis. |
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المؤلفون: | Juneja SC; Arthritis Program, Orthopaedic Surgery, Toronto Western Hospital, 399 Bathurst Street, Toronto, ON, Canada M5T 2S8., Viswanathan S; Institute of Biomaterials and Biomedical Engineering, University of Toronto, 164 College Street, Toronto, ON, Canada M5S 3G9; Krembil Research Institute, University Health Network, 399 Bathurst Street, Toronto, ON, Canada M5T 2S8., Ganguly M; STTARR Innovation Centre, Princess Margaret Cancer Centre, University Health Network, 101 College Street, Toronto, ON, Canada M5G 1L7., Veillette C; Arthritis Program, Orthopaedic Surgery, Toronto Western Hospital, 399 Bathurst Street, Toronto, ON, Canada M5T 2S8. |
المصدر: | Bone marrow research [Bone Marrow Res] 2016; Vol. 2016, pp. 3152065. Date of Electronic Publication: 2016 Feb 11. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Hindawi Pub. Corp Country of Publication: Egypt NLM ID: 101566202 Publication Model: Print-Electronic Cited Medium: Print ISSN: 2090-2999 (Print) Linking ISSN: 20903006 NLM ISO Abbreviation: Bone Marrow Res Subsets: PubMed not MEDLINE |
أسماء مطبوعة: | Original Publication: Cairo : Hindawi Pub. Corp. |
مستخلص: | The procedure for aspiration of bone marrow from the femur of patients undergoing total knee arthroplasty (TKA) or total hip arthroplasty (THA) may vary from an OR (operating room) to OR based on the surgeon's skill and may lead to varied extent of clotting of the marrow and this, in turn, presents difficulty in the isolation of mesenchymal stem cells (MSCs) from such clotted bone marrow. We present a simple detailed protocol for aspirating bone marrow from such patients, isolation, and characterization of MSCs from the aspirated bone marrow specimens and show that the bone marrow presented no clotting or exhibited minimal clotting. This represents an economical source and convenient source of MSCs from bone marrow for use in regenerative medicine. Also, we presented the detailed protocol and showed that the MSCs derived from such bone marrow specimens exhibited MSCs characteristics and generated micromass cartilages, the recipe for regenerative medicine for osteoarthritis. The protocols we presented can be used as standard operating procedures (SOPs) by researchers and clinicians. |
References: | Cell Tissue Bank. 2015 Jun;16(2):195-207. (PMID: 25204398) Arthritis Res Ther. 2007;9(1):204. (PMID: 17316462) Stem Cell Res Ther. 2010 Apr 02;1(1):8. (PMID: 20504289) Dev Biol. 2014 Apr 1;388(1):1-10. (PMID: 24509075) Cell Res. 2002 Mar;12(1):19-32. (PMID: 11942407) Arthritis Rheum. 2008 May;58(5):1377-88. (PMID: 18438858) Ann Rheum Dis. 1965 Mar;24:123-35. (PMID: 14293671) J Orthop Res. 1998 Jan;16(1):96-9. (PMID: 9565079) Ann Rheum Dis. 1965 Mar;24:136-46. (PMID: 14293672) Blood. 2008 Mar 1;111(5):2631-5. (PMID: 18086871) Injury. 2014 Oct;45 Suppl 4:S42-7. (PMID: 25384474) Methods Mol Biol. 2011;698:253-78. (PMID: 21431525) J Cell Sci. 1992 Dec;103 ( Pt 4):1101-10. (PMID: 1487492) Eur Cell Mater. 2014 Mar 25;27:237-50; discussion 249-50. (PMID: 24668596) Calif Med. 1952 Aug;77(2):138-9. (PMID: 12978895) Osteoarthritis Cartilage. 2013 Nov;21(11):1790-8. (PMID: 23850530) Osteoarthritis Cartilage. 2004 Apr;12(4):306-13. (PMID: 15023382) Ann Rheum Dis. 1968 Nov;27(6):544-58. (PMID: 5728099) J Rheumatol. 1987 May;14 Spec No:25-7. (PMID: 3625672) Biotechnol Lett. 2010 Sep;32(9):1339-46. (PMID: 20464452) J Histochem Cytochem. 2009 Oct;57(10):923-31. (PMID: 19546472) Cytotherapy. 2006;8(4):315-7. (PMID: 16923606) Cell Tissue Res. 2005 Nov;322(2):217-26. (PMID: 16091918) J Cell Sci. 2003 Jul 15;116(Pt 14):2949-55. (PMID: 12783985) Blood. 1991 Jul 1;78(1):55-62. (PMID: 2070060) Osteoarthritis Cartilage. 2013 Jan;21(1):246-58. (PMID: 23085560) Stem Cell Reports. 2015 Mar 10;4(3):459-72. (PMID: 25733021) Percept Mot Skills. 2004 Feb;98(1):116-22. (PMID: 15058874) Semin Arthritis Rheum. 1991 Dec;21(3 Suppl 2):2-11. (PMID: 1796302) Science. 1999 Apr 2;284(5411):143-7. (PMID: 10102814) Osteoarthritis Cartilage. 2002 Jan;10(1):13-22. (PMID: 11795979) J Anat. 1996 Aug;189 ( Pt 1):9-22. (PMID: 8771392) Stem Cells Dev. 2004 Aug;13(4):436-48. (PMID: 15345137) Kathmandu Univ Med J (KUMJ). 2013 Jul-Sep;11(43):221-5. (PMID: 24442170) Biol Blood Marrow Transplant. 2002;8(4):213-20. (PMID: 12014810) |
تواريخ الأحداث: | Date Created: 20160409 Date Completed: 20160408 Latest Revision: 20200930 |
رمز التحديث: | 20240628 |
مُعرف محوري في PubMed: | PMC4766320 |
DOI: | 10.1155/2016/3152065 |
PMID: | 27057356 |
قاعدة البيانات: | MEDLINE |
تدمد: | 2090-2999 |
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DOI: | 10.1155/2016/3152065 |