دورية أكاديمية
Increased ribosome density associated to positively charged residues is evident in ribosome profiling experiments performed in the absence of translation inhibitors.
| العنوان: | Increased ribosome density associated to positively charged residues is evident in ribosome profiling experiments performed in the absence of translation inhibitors. |
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| المؤلفون: | Requião RD; a Programa de Biologia Estrutural, Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro , Rio de Janeiro , RJ , Brazil., de Souza HJ; b Programa de Pós-Graduação em Informática, Universidade Federal do Rio de Janeiro , Rio de Janeiro , RJ , Brazil., Rossetto S; b Programa de Pós-Graduação em Informática, Universidade Federal do Rio de Janeiro , Rio de Janeiro , RJ , Brazil., Domitrovic T; c Departamento de Virologia , Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro , Rio de Janeiro , Brazil., Palhano FL; a Programa de Biologia Estrutural, Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro , Rio de Janeiro , RJ , Brazil. |
| المصدر: | RNA biology [RNA Biol] 2016 Jun 02; Vol. 13 (6), pp. 561-8. Date of Electronic Publication: 2016 Apr 11. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Taylor & Francis Country of Publication: United States NLM ID: 101235328 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1555-8584 (Electronic) Linking ISSN: 15476286 NLM ISO Abbreviation: RNA Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2015- : Philadelphia, PA : Taylor & Francis Original Publication: Georgetown, TX : Landes Bioscience, |
| مواضيع طبية MeSH: | RNA, Messenger/*genetics , Ribosomes/*metabolism , Saccharomyces cerevisiae/*genetics , Sequence Analysis, RNA/*methods, Protein Biosynthesis ; Protein Synthesis Inhibitors/pharmacology ; RNA, Fungal/genetics ; RNA, Fungal/metabolism ; RNA, Messenger/metabolism ; Saccharomyces cerevisiae/metabolism |
| مستخلص: | It has been proposed that polybasic peptides cause slower movement of ribosomes through an electrostatic interaction with the highly negative ribosome exit tunnel. Ribosome profiling data-the sequencing of short ribosome-bound fragments of mRNA-is a powerful tool for the analysis of mRNA translation. Using the yeast Saccharomyces cerevisiae as a model, we showed that reduced translation efficiency associated with polybasic protein sequences could be inferred from ribosome profiling. However, an increase in ribosome density at polybasic sequences was evident only when the commonly used translational inhibitors cycloheximide and anisomycin were omitted during mRNA isolation. Since ribosome profiling performed without inhibitors agrees with experimental evidence obtained by other methods, we conclude that cycloheximide and anisomycin must be avoided in ribosome profiling experiments. |
| References: | Genome Res. 2014 Mar;24(3):422-30. (PMID: 24318730) Science. 2009 Apr 10;324(5924):218-23. (PMID: 19213877) Mol Syst Biol. 2014 Dec 23;10:770. (PMID: 25538139) Mol Cell Biol. 2014 Nov;34(21):4062-76. (PMID: 25154418) Cell. 2012 Nov 21;151(5):1042-54. (PMID: 23178123) Annu Rev Biophys. 2011;40:337-59. (PMID: 21370971) Cell. 2015 Jun 18;161(7):1606-18. (PMID: 26052047) BMC Genomics. 2015;16 Suppl 10:S5. (PMID: 26449596) Nat Protoc. 2012 Jul 26;7(8):1534-50. (PMID: 22836135) J Mol Biol. 2007 Aug 31;371(5):1378-91. (PMID: 17631312) Cold Spring Harb Perspect Biol. 2012 Dec 01;4(12):null. (PMID: 23209153) Nature. 2010 Sep 23;467(7314):470-3. (PMID: 20835226) RNA. 2010 Dec;16(12):2516-28. (PMID: 20971810) Nucleic Acids Res. 2014;42(17):e134. (PMID: 25056308) J Biol Chem. 2009 Apr 17;284(16):10343-52. (PMID: 19204001) J Mol Biol. 2008 Dec 5;384(1):73-86. (PMID: 18822297) Cell. 2011 Nov 11;147(4):789-802. (PMID: 22056041) Cell. 2010 Apr 16;141(2):344-54. (PMID: 20403328) Science. 2015 Jan 2;347(6217):75-8. (PMID: 25554787) Genes Dev. 2007 Mar 1;21(5):519-24. (PMID: 17344413) Sci Adv. 2015 Jul;1(6):null. (PMID: 26322332) Elife. 2015 Feb 19;4:null. (PMID: 25695637) Genome Res. 2014 Dec;24(12):2011-21. (PMID: 25294246) Cell Rep. 2016 Feb 23;14 (7):1787-99. (PMID: 26876183) Elife. 2014 May 09;3:e01257. (PMID: 24842990) PLoS Biol. 2013;11(3):e1001508. (PMID: 23554576) PLoS Genet. 2015 Dec 11;11(12):e1005732. (PMID: 26656907) Proc Natl Acad Sci U S A. 2014 Nov 11;111(45):15981-6. (PMID: 25349383) |
| فهرسة مساهمة: | Keywords: Cycloheximide; polyA sequences; polybasic sequences; ribosome profiling; ribosome translation |
| المشرفين على المادة: | 0 (Protein Synthesis Inhibitors) 0 (RNA, Fungal) 0 (RNA, Messenger) |
| تواريخ الأحداث: | Date Created: 20160412 Date Completed: 20171011 Latest Revision: 20220321 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC4962802 |
| DOI: | 10.1080/15476286.2016.1172755 |
| PMID: | 27064519 |
| قاعدة البيانات: | MEDLINE |
PDF full text from Publisher | تدمد: | 1555-8584 |
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| DOI: | 10.1080/15476286.2016.1172755 |