دورية أكاديمية
أعرض في EDS

Oligosaccharides Released from Milk Glycoproteins Are Selective Growth Substrates for Infant-Associated Bifidobacteria.

التفاصيل البيبلوغرافية
العنوان: Oligosaccharides Released from Milk Glycoproteins Are Selective Growth Substrates for Infant-Associated Bifidobacteria.
المؤلفون: Karav S; Department of Food Science and Technology, University of California, Davis, California, USA., Le Parc A; Department of Food Science and Technology, University of California, Davis, California, USA., Leite Nobrega de Moura Bell JM; Department of Food Science and Technology, University of California, Davis, California, USA., Frese SA; Department of Food Science and Technology, University of California, Davis, California, USA.; Foods for Health Institute, University of California, Davis, California, USA., Kirmiz N; Department of Food Science and Technology, University of California, Davis, California, USA., Block DE; Department of Viticulture & Enology, University of California, Davis, California, USA.; Department of Chemical Engineering and Materials Science, University of California, Davis, California, USA., Barile D; Department of Food Science and Technology, University of California, Davis, California, USA.; Foods for Health Institute, University of California, Davis, California, USA., Mills DA; Department of Food Science and Technology, University of California, Davis, California, USA damills@ucdavis.edu.; Foods for Health Institute, University of California, Davis, California, USA.; Department of Viticulture & Enology, University of California, Davis, California, USA.
المصدر: Applied and environmental microbiology [Appl Environ Microbiol] 2016 May 31; Vol. 82 (12), pp. 3622-3630. Date of Electronic Publication: 2016 May 31 (Print Publication: 2016).
نوع المنشور: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: American Society for Microbiology Country of Publication: United States NLM ID: 7605801 Publication Model: Electronic-Print Cited Medium: Internet ISSN: 1098-5336 (Electronic) Linking ISSN: 00992240 NLM ISO Abbreviation: Appl Environ Microbiol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Washington, American Society for Microbiology.
مواضيع طبية MeSH: Bifidobacterium/*enzymology , Bifidobacterium/*metabolism , Glycoproteins/*metabolism , Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase/*metabolism , Milk/*metabolism , Oligosaccharides/*metabolism, Animals ; Bifidobacterium/growth & development ; Humans ; Infant
مستخلص: Unlabelled: Milk, in addition to nourishing the neonate, provides a range of complex glycans whose construction ensures a specific enrichment of key members of the gut microbiota in the nursing infant, a consortium known as the milk-oriented microbiome. Milk glycoproteins are thought to function similarly, as specific growth substrates for bifidobacteria common to the breast-fed infant gut. Recently, a cell wall-associated endo-β-N-acetylglucosaminidase (EndoBI-1) found in various infant-borne bifidobacteria was shown to remove a range of intact N-linked glycans. We hypothesized that these released oligosaccharide structures can serve as a sole source for the selective growth of bifidobacteria. We demonstrated that EndoBI-1 released N-glycans from concentrated bovine colostrum at the pilot scale. EndoBI-1-released N-glycans supported the rapid growth of Bifidobacterium longum subsp. infantis (B. infantis), a species that grows well on human milk oligosaccharides, but did not support growth of Bifidobacterium animalis subsp. lactis (B. lactis), a species which does not. Conversely, B. infantis ATCC 15697 did not grow on the deglycosylated milk protein fraction, clearly demonstrating that the glycan portion of milk glycoproteins provided the key substrate for growth. Mass spectrometry-based profiling revealed that B. infantis consumed 73% of neutral and 92% of sialylated N-glycans, while B. lactis degraded only 11% of neutral and virtually no (<1%) sialylated N-glycans. These results provide mechanistic support that N-linked glycoproteins from milk serve as selective substrates for the enrichment of infant-associated bifidobacteria capable of carrying out the initial deglycosylation. Moreover, released N-glycans were better growth substrates than the intact milk glycoproteins, suggesting that EndoBI-1 cleavage is a key initial step in consumption of glycoproteins. Finally, the variety of N-glycans released from bovine milk glycoproteins suggests that they may serve as novel prebiotic substrates with selective properties similar to those of human milk oligosaccharides.
Importance: It has been previously shown that glycoproteins serve as growth substrates for bifidobacteria. However, which part of a glycoprotein (glycans or polypeptides) is responsible for this function was not known. In this study, we used a novel enzyme to cleave conjugated N-glycans from milk glycoproteins and tested their consumption by various bifidobacteria. The results showed that the glycans selectively stimulated the growth of B. infantis, which is a key infant gut microbe. The selectivity of consumption of individual N-glycans was determined using advanced mass spectrometry (nano-liquid chromatography chip-quadrupole time of flight mass spectrometry [nano-LC-Chip-Q-TOF MS]) to reveal that B. infantis can consume the range of glycan structures released from whey protein concentrate.
(Copyright © 2016, American Society for Microbiology. All Rights Reserved.)
References: BMC Microbiol. 2014 Nov 25;14:282. (PMID: 25420416)
Science. 2005 Mar 25;307(5717):1955-9. (PMID: 15790854)
Annu Rev Nutr. 2014;34:143-69. (PMID: 24850388)
J Biochem. 2001 Jun;129(6):923-8. (PMID: 11388907)
Electrophoresis. 2014 Jun;35(11):1560-70. (PMID: 24519758)
J Pediatr. 2013 Dec;163(6):1585-1591.e9. (PMID: 23993139)
Proc Natl Acad Sci U S A. 2010 Nov 9;107(45):19514-9. (PMID: 20974960)
Enzyme Microb Technol. 2015 Sep;77:46-53. (PMID: 26138399)
J Proteome Res. 2012 May 4;11(5):2912-24. (PMID: 22439776)
PLoS One. 2011 Mar 15;6(3):e17315. (PMID: 21423604)
Biometals. 2004 Jun;17(3):279-83. (PMID: 15222478)
J Biol Chem. 2013 Aug 30;288(35):25194-206. (PMID: 23843461)
J Biol Chem. 2009 Jul 3;284(27):18445-57. (PMID: 19403529)
PLoS Pathog. 2011 Jun;7(6):e1002118. (PMID: 21738475)
J Proteome Res. 2015 Jan 2;14(1):491-502. (PMID: 25300177)
Biotechnol Prog. 2015 Sep-Oct;31(5):1331-9. (PMID: 26097235)
J Biol Chem. 2004 May 21;279(21):22558-70. (PMID: 15028731)
Nature. 2013 Oct 3;502(7469):96-9. (PMID: 23995682)
Electrophoresis. 2014 Jun;35(11):1742-50. (PMID: 24519724)
J Agric Food Chem. 2011 Nov 23;59(22):11949-55. (PMID: 22004447)
Mol Cell Proteomics. 2012 Sep;11(9):775-85. (PMID: 22745059)
J Bacteriol. 2008 Jan;190(1):221-30. (PMID: 17981977)
Electrophoresis. 2012 Mar;33(5):797-814. (PMID: 22522536)
Microb Biotechnol. 2009 May;2(3):333-42. (PMID: 21261928)
Front Microbiol. 2015 Sep 24;6:1030. (PMID: 26441950)
Appl Environ Microbiol. 2012 Feb;78(3):795-803. (PMID: 22138995)
Glycobiology. 2012 Mar;22(3):361-8. (PMID: 21926104)
Microbiology. 2013 Apr;159(Pt 4):649-64. (PMID: 23460033)
PLoS One. 2013 Oct 25;8(10):e76341. (PMID: 24204617)
PLoS One. 2014 Mar 07;9(3):e91035. (PMID: 24608122)
J Biol Chem. 2011 Oct 7;286(40):34583-92. (PMID: 21832085)
Adv Nutr. 2011 May;2(3):284-9. (PMID: 22332060)
J Biol Chem. 2012 Jan 2;287(1):693-700. (PMID: 22090027)
Glycobiology. 1999 May;9(5):469-79. (PMID: 10207179)
Appl Environ Microbiol. 2008 Mar;74(6):1936-40. (PMID: 18223105)
J Bacteriol. 2006 Feb;188(4):1260-5. (PMID: 16452407)
J Agric Food Chem. 2007 Oct 31;55(22):8914-9. (PMID: 17915960)
Mol Nutr Food Res. 2007 Nov;51(11):1398-405. (PMID: 17966141)
Sci Rep. 2015 Sep 04;5:13517. (PMID: 26337101)
J Agric Food Chem. 2010 May 12;58(9):5334-40. (PMID: 20394371)
Annu Rev Nutr. 2000;20:699-722. (PMID: 10940350)
Biotechnol Prog. 2015 Sep-Oct;31(5):1323-30. (PMID: 26101185)
J Proteome Res. 2010 Aug 6;9(8):4138-51. (PMID: 20578730)
Eur J Biochem. 2002 Jan;269(2):712-8. (PMID: 11856332)
Anaerobe. 2012 Aug;18(4):430-5. (PMID: 22579845)
Biochem J. 1995 Nov 15;312 ( Pt 1):107-14. (PMID: 7492299)
J Dairy Sci. 2010 Sep;93(9):3940-9. (PMID: 20723667)
J Agric Food Chem. 2011 Apr 27;59(8):4255-63. (PMID: 21384928)
J Proteome Res. 2011 Feb 4;10(2):856-68. (PMID: 21133381)
J Med Microbiol. 1999 Jun;48(6):541-9. (PMID: 10359303)
J Dairy Sci. 2008 Oct;91(10):3768-78. (PMID: 18832198)
J Biol Chem. 2011 Apr 8;286(14):11909-18. (PMID: 21288901)
Nat Rev Mol Cell Biol. 2012 Jun 22;13(7):448-62. (PMID: 22722607)
EMBO J. 2001 Jun 15;20(12):3046-55. (PMID: 11406581)
Appl Environ Microbiol. 2013 Oct;79(19):6040-9. (PMID: 23892749)
معلومات مُعتمدة: F32 AT008533 United States AT NCCIH NIH HHS; R01 AT007079 United States AT NCCIH NIH HHS; R01 AT008759 United States AT NCCIH NIH HHS
المشرفين على المادة: 0 (Glycoproteins)
0 (Oligosaccharides)
EC 3.2.1.96 (Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase)
تواريخ الأحداث: Date Created: 20160417 Date Completed: 20170918 Latest Revision: 20181113
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC4959171
DOI: 10.1128/AEM.00547-16
PMID: 27084007
قاعدة البيانات: MEDLINE
الوصف
تدمد:1098-5336
DOI:10.1128/AEM.00547-16