دورية أكاديمية
Antimicrobial photodynamic effect of extracts and oxoaporphine alkaloid isomoschatoline from Guatteria blepharophylla.
| العنوان: | Antimicrobial photodynamic effect of extracts and oxoaporphine alkaloid isomoschatoline from Guatteria blepharophylla. |
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| المؤلفون: | Andreazza NL; Departamento de Biologia Vegetal, PPG BTPB and PPG BV, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), 13083-970 Campinas, SP, Brazil. Electronic address: nathilu@gmail.com., Caramano de Lourenço C; Departamento de Biologia Vegetal, PPG BTPB and PPG BV, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), 13083-970 Campinas, SP, Brazil., Hernandez-Tasco ÁJ; Departamento de Biologia Vegetal, PPG BTPB and PPG BV, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), 13083-970 Campinas, SP, Brazil., Pinheiro ML; Departamento de Quıímica, Universidade Federal do Amazonas, Mini-Campus, Avenida General Rodrigo Otávio Jordão Ramos, 3000, Coroado, 69077-000 Manaus, AM, Brazil., Alves Stefanello MÉ; Departamento de Química, Universidade Federal do Paraná (UFPR), 81531-990 Curitiba, PR, Brazil., Vilaça Costa E; Departamento de Quıímica, Universidade Federal do Amazonas, Mini-Campus, Avenida General Rodrigo Otávio Jordão Ramos, 3000, Coroado, 69077-000 Manaus, AM, Brazil., Salvador MJ; Departamento de Biologia Vegetal, PPG BTPB and PPG BV, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), 13083-970 Campinas, SP, Brazil. Electronic address: marcosjs@unicamp.br. |
| المصدر: | Journal of photochemistry and photobiology. B, Biology [J Photochem Photobiol B] 2016 Jul; Vol. 160, pp. 154-62. Date of Electronic Publication: 2016 Apr 14. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Sequoia Country of Publication: Switzerland NLM ID: 8804966 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2682 (Electronic) Linking ISSN: 10111344 NLM ISO Abbreviation: J Photochem Photobiol B Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Lausanne : Elsevier Sequoia, 1987- |
| مواضيع طبية MeSH: | Alkaloids/*pharmacology , Anti-Bacterial Agents/*pharmacology , Aporphines/*pharmacology , Guatteria/*chemistry , Plant Extracts/*pharmacology, Microbial Sensitivity Tests ; Spectrometry, Fluorescence |
| مستخلص: | Photodynamic Therapy, a tumor therapy idealized at the beginning of the last century, emerges nowadays as a promising treatment alternative against infectious diseases. In this study we report a bioguided study of Guatteria blepharophylla phytoderivatives for antimicrobial PDT. Crude extracts and fraction from the species bark were obtained and further fractionated for substances isolation. All samples were evaluated in relation to their photophysical (absorbance and fluorescence) and photochemical properties (1,3-DPBF bleaching method). Then, bioassays were conducted using as biological models bacteria and yeast strains and a diode laser as a light source. Phytochemical analyses lead to the isolation of 5 isoquinoline alkaloids from oxoaporphine subclass, denominated GB1 to GB5. Photophysical and photochemical analysis showed that extracts, fraction and GB1 (isomoschatoline) presented absorption profile with bands at 600-700nm and were positive for singlet oxygen production. Photobiological assays indicate that these samples presented photodynamic antimicrobial activity against both gram-positive and gram-negative bacterial and some Candida ssp. yeast strains at sub-inhibitory concentrations. The susceptibility of gram-negative bacteria was significantly enhanced when CaCl2 or MgCl2 were employed. Greater energy doses and double sample's dosage also decreased microbial survival. It is suggested that GB1 photodynamic activity happens through both types I and II photochemical mechanisms, but with a predominance of the latter. Phytoderivatives of G. blepharophylla promoted antimicrobial effect, however more detailed study concerning chemical composition of the crude extracts and fractions as also photophysical and photochemical characteristics of GB1 are necessary to ensure their potential as photosensitizers at antimicrobial photodynamic inactivation. (Copyright © 2016 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Antimicrobial photodynamic therapy; Antimicrobial resistance; Guatteria blepharophylla; Isoquinoline alkaloids; Oxoaporphine alkaloid isomoschatoline |
| المشرفين على المادة: | 0 (Alkaloids) 0 (Anti-Bacterial Agents) 0 (Aporphines) 0 (Plant Extracts) 13NS2KTD6H (aporphine) |
| تواريخ الأحداث: | Date Created: 20160424 Date Completed: 20170227 Latest Revision: 20171116 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1016/j.jphotobiol.2016.04.010 |
| PMID: | 27107335 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-2682 |
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| DOI: | 10.1016/j.jphotobiol.2016.04.010 |