دورية أكاديمية
أعرض في EDS

Skin penetration and dermal tolerability of acrylic nanocapsules: Influence of the surface charge and a chitosan gel used as vehicle.

التفاصيل البيبلوغرافية
العنوان: Skin penetration and dermal tolerability of acrylic nanocapsules: Influence of the surface charge and a chitosan gel used as vehicle.
المؤلفون: Contri RV; Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil., Fiel LA; Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil., Alnasif N; Institut für Pharmazie (Pharmakologie und Toxikologie), Freie Universität Berlin, Berlin, Germany., Pohlmann AR; Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil; Instituto de Química, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil., Guterres SS; Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil., Schäfer-Korting M; Institut für Pharmazie (Pharmakologie und Toxikologie), Freie Universität Berlin, Berlin, Germany. Electronic address: monika.schaefer-korting@fu-berlin.de.
المصدر: International journal of pharmaceutics [Int J Pharm] 2016 Jun 30; Vol. 507 (1-2), pp. 12-20. Date of Electronic Publication: 2016 Apr 26.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier/North-Holland Biomedical Press Country of Publication: Netherlands NLM ID: 7804127 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-3476 (Electronic) Linking ISSN: 03785173 NLM ISO Abbreviation: Int J Pharm Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Amsterdam, Elsevier/North-Holland Biomedical Press.
مواضيع طبية MeSH: Chitosan/*administration & dosage , Chitosan/*chemistry , Nanocapsules/*administration & dosage , Nanocapsules/*chemistry , Skin Absorption/*drug effects, Cell Survival/drug effects ; Cells, Cultured ; Chitosan/adverse effects ; Chitosan/pharmacokinetics ; Dermis/metabolism ; Epidermis/metabolism ; Fibroblasts/drug effects ; Fibroblasts/metabolism ; Gels/administration & dosage ; Gels/adverse effects ; Gels/chemistry ; Humans ; Keratinocytes/drug effects ; Keratinocytes/metabolism ; Nanocapsules/adverse effects ; Particle Size ; Polymethacrylic Acids/administration & dosage ; Polymethacrylic Acids/adverse effects ; Polymethacrylic Acids/chemistry ; Polysorbates/administration & dosage ; Polysorbates/adverse effects ; Polysorbates/chemistry ; Surface Properties
مستخلص: For an improved understanding of the relevant particle features for cutaneous use, we studied the effect of the surface charge of acrylic nanocapsules (around 150nm) and the effect of a chitosan gel vehicle on the particle penetration into normal and stripped human skin ex vivo as well as local tolerability (cytotoxicity and irritancy). Rhodamin-tagged nanocapsules penetrated and remained in the stratum corneum. Penetration of cationic nanocapsules exceeded the penetration of anionic nanocapsules. When applied on stripped skin, however, the fluorescence was also recorded in the viable epidermis and dermis. Cationic surface charge and embedding the particles into chitosan gel favored access to deeper skin. Keratinocytes took up the nanocapsules rapidly. Cytotoxicity (viability<80%), following exposure for ≥24h, appears to be due to the surfactant polysorbate 80, used for nanocapsuleś stabilization. Uptake by fibroblasts was low and no cytotoxicity was observed. No irritant reactions were detected in the HET-CAM test. In conclusion, the surface charge and chitosan vehicle, as well as the skin barrier integrity, influence the skin penetration of acrylic nanocapsules. Particle localization in the intact stratum corneum of normal skin and good tolerability make the nanocapsules candidates for topical use on the skin, provided that the polymer wall allows the release of the active encapsulated substance.
(Copyright © 2016 Elsevier B.V. All rights reserved.)
فهرسة مساهمة: Keywords: Chitosan; Cytotoxicity; Irritancy; Nanocapsules; Polymer; Skin penetration
المشرفين على المادة: 0 (Gels)
0 (Nanocapsules)
0 (Polymethacrylic Acids)
0 (Polysorbates)
25086-15-1 (methylmethacrylate-methacrylic acid copolymer)
9012-76-4 (Chitosan)
تواريخ الأحداث: Date Created: 20160501 Date Completed: 20170718 Latest Revision: 20170718
رمز التحديث: 20231215
DOI: 10.1016/j.ijpharm.2016.03.046
PMID: 27130364
قاعدة البيانات: MEDLINE
الوصف
تدمد:1873-3476
DOI:10.1016/j.ijpharm.2016.03.046