دورية أكاديمية
أعرض في EDS

Exopolysaccharide microchannels direct bacterial motility and organize multicellular behavior.

التفاصيل البيبلوغرافية
العنوان: Exopolysaccharide microchannels direct bacterial motility and organize multicellular behavior.
المؤلفون: Berleman JE; Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.; Department of Biology, St Mary's College, Moraga, CA, USA.; Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA., Zemla M; Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA., Remis JP; Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA., Liu H; Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA.; State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, Jinan, China., Davis AE; Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA., Worth AN; Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.; Department of Biology, St Mary's College, Moraga, CA, USA., West Z; Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.; Department of Biology, St Mary's College, Moraga, CA, USA., Zhang A; Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA., Park H; Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA., Bosneaga E; Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA., van Leer B; FEI, Inc, Hillsboro, OR, USA., Tsai W; Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA., Zusman DR; Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA., Auer M; Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.
المصدر: The ISME journal [ISME J] 2016 Nov; Vol. 10 (11), pp. 2620-2632. Date of Electronic Publication: 2016 May 06.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Oxford University Press Country of Publication: England NLM ID: 101301086 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1751-7370 (Electronic) Linking ISSN: 17517362 NLM ISO Abbreviation: ISME J Subsets: MEDLINE
أسماء مطبوعة: Publication: 2024- : Oxford : Oxford University Press
Original Publication: London : Nature Pub. Group
مواضيع طبية MeSH: Myxococcus xanthus/*cytology , Myxococcus xanthus/*metabolism , Polysaccharides, Bacterial/*metabolism, Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Biofilms ; Cell Membrane/genetics ; Cell Membrane/metabolism ; Myxococcus xanthus/genetics
مستخلص: The myxobacteria are a family of soil bacteria that form biofilms of complex architecture, aligned multilayered swarms or fruiting body structures that are simple or branched aggregates containing myxospores. Here, we examined the structural role of matrix exopolysaccharide (EPS) in the organization of these surface-dwelling bacterial cells. Using time-lapse light and fluorescence microscopy, as well as transmission electron microscopy and focused ion beam/scanning electron microscopy (FIB/SEM) electron microscopy, we found that Myxococcus xanthus cell organization in biofilms is dependent on the formation of EPS microchannels. Cells are highly organized within the three-dimensional structure of EPS microchannels that are required for cell alignment and advancement on surfaces. Mutants lacking EPS showed a lack of cell orientation and poor colony migration. Purified, cell-free EPS retains a channel-like structure, and can complement EPS - mutant motility defects. In addition, EPS provides the cooperative structure for fruiting body formation in both the simple mounds of M. xanthus and the complex, tree-like structures of Chondromyces crocatus. We furthermore investigated the possibility that EPS impacts community structure as a shared resource facilitating cooperative migration among closely related isolates of M. xanthus.
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معلومات مُعتمدة: P01 GM051487 United States GM NIGMS NIH HHS; R01 GM020509 United States GM NIGMS NIH HHS
المشرفين على المادة: 0 (Bacterial Proteins)
0 (Polysaccharides, Bacterial)
تواريخ الأحداث: Date Created: 20160507 Date Completed: 20170918 Latest Revision: 20181202
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC5113850
DOI: 10.1038/ismej.2016.60
PMID: 27152937
قاعدة البيانات: MEDLINE
الوصف
تدمد:1751-7370
DOI:10.1038/ismej.2016.60