دورية أكاديمية
Calpain-5 Expression in the Retina Localizes to Photoreceptor Synapses.
العنوان: | Calpain-5 Expression in the Retina Localizes to Photoreceptor Synapses. |
---|---|
المؤلفون: | Schaefer KA; Omics Laboratory, University of Iowa, Iowa City, Iowa, United States 2Department of Ophthalmology & Visual Sciences, University of Iowa, Iowa City, Iowa, United States., Toral MA; Omics Laboratory, University of Iowa, Iowa City, Iowa, United States 2Department of Ophthalmology & Visual Sciences, University of Iowa, Iowa City, Iowa, United States 3Medical Scientist Training Program, University of Iowa, Iowa City, Iowa, United States., Velez G; Omics Laboratory, University of Iowa, Iowa City, Iowa, United States 2Department of Ophthalmology & Visual Sciences, University of Iowa, Iowa City, Iowa, United States 3Medical Scientist Training Program, University of Iowa, Iowa City, Iowa, United States., Cox AJ; Department of Pediatrics, University of Iowa, Iowa City, Iowa, United States., Baker SA; Department of Ophthalmology & Visual Sciences, University of Iowa, Iowa City, Iowa, United States 5Department of Biochemistry, University of Iowa, Iowa City, Iowa, United States., Borcherding NC; Omics Laboratory, University of Iowa, Iowa City, Iowa, United States 3Medical Scientist Training Program, University of Iowa, Iowa City, Iowa, United States., Colgan DF; Omics Laboratory, University of Iowa, Iowa City, Iowa, United States 2Department of Ophthalmology & Visual Sciences, University of Iowa, Iowa City, Iowa, United States., Bondada V; Spinal Cord and Brain Injury Research Center, University of Kentucky, Lexington, Kentucky, United States., Mashburn CB; Spinal Cord and Brain Injury Research Center, University of Kentucky, Lexington, Kentucky, United States., Yu CG; Spinal Cord and Brain Injury Research Center, University of Kentucky, Lexington, Kentucky, United States., Geddes JW; Spinal Cord and Brain Injury Research Center, University of Kentucky, Lexington, Kentucky, United States., Tsang SH; Barbara & Donald Jonas Stem Cell Laboratory, and Bernard & Shirlee Brown Glaucoma Laboratory, Department of Pathology & Cell Biology, Institute of Human Nutrition, College of Physicians and Surgeons, Columbia University, New York, New York, United States., Bassuk AG; Department of Pediatrics, University of Iowa, Iowa City, Iowa, United States 9Neurology, University of Iowa, Iowa City, Iowa, United States., Mahajan VB; Omics Laboratory, University of Iowa, Iowa City, Iowa, United States 2Department of Ophthalmology & Visual Sciences, University of Iowa, Iowa City, Iowa, United States. |
المصدر: | Investigative ophthalmology & visual science [Invest Ophthalmol Vis Sci] 2016 May 01; Vol. 57 (6), pp. 2509-21. |
نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
اللغة: | English |
بيانات الدورية: | Publisher: Association For Research In Vision And Ophthalmology (Arvo) Country of Publication: United States NLM ID: 7703701 Publication Model: Print Cited Medium: Internet ISSN: 1552-5783 (Electronic) Linking ISSN: 01460404 NLM ISO Abbreviation: Invest Ophthalmol Vis Sci Subsets: MEDLINE |
أسماء مطبوعة: | Publication: Brookline Ma : Association For Research In Vision And Ophthalmology (Arvo) Original Publication: St. Louis, Mosby. |
مواضيع طبية MeSH: | Gene Expression Regulation, Neoplastic*, Calpain/*genetics , Photoreceptor Cells/*metabolism , RNA, Neoplasm/*genetics , Retinal Neoplasms/*genetics , Retinoblastoma/*genetics , Synapses/*metabolism, Animals ; Blotting, Western ; Calpain/biosynthesis ; Cattle ; Female ; Humans ; Immunohistochemistry ; Male ; Mice ; Neoplasms, Experimental ; Photoreceptor Cells/pathology ; Retina/metabolism ; Retina/pathology ; Retinal Neoplasms/metabolism ; Retinal Neoplasms/pathology ; Retinoblastoma/metabolism ; Retinoblastoma/pathology ; Tumor Cells, Cultured |
مستخلص: | Purpose: We characterize calpain-5 (CAPN5) expression in retinal and neuronal subcellular compartments. Methods: CAPN5 gene variants were classified using the exome variant server, and RNA-sequencing was used to compare expression of CAPN5 mRNA in the mouse and human retina and in retinoblastoma cells. Expression of CAPN5 protein was ascertained in humans and mice in silico, in mouse retina by immunohistochemistry, and in neuronal cancer cell lines and fractionated central nervous system tissue extracts by Western analysis with eight antibodies targeting different CAPN5 regions. Results: Most CAPN5 genetic variation occurs outside its protease core; and searches of cancer and epilepsy/autism genetic databases found no variants similar to hyperactivating retinal disease alleles. The mouse retina expressed one transcript for CAPN5 plus those of nine other calpains, similar to the human retina. In Y79 retinoblastoma cells, the level of CAPN5 transcript was very low. Immunohistochemistry detected CAPN5 expression in the inner and outer nuclear layers and at synapses in the outer plexiform layer. Western analysis of fractionated retinal extracts confirmed CAPN5 synapse localization. Western blots of fractionated brain neuronal extracts revealed distinct subcellular patterns and the potential presence of autoproteolytic CAPN5 domains. Conclusions: CAPN5 is moderately expressed in the retina and, despite higher expression in other tissues, hyperactive disease mutants of CAPN5 only manifest as eye disease. At the cellular level, CAPN5 is expressed in several different functional compartments. CAPN5 localization at the photoreceptor synapse and with mitochondria explains the neural circuitry phenotype in human CAPN5 disease alleles. |
References: | Brain Res. 2005 Jul 19;1050(1-2):148-55. (PMID: 15979593) Proc Natl Acad Sci U S A. 2010 Mar 30;107(13):6022-7. (PMID: 20231455) Clin Ophthalmol. 2013;7:1339-45. (PMID: 23861576) J Child Neurol. 2009 Mar;24(3):346-8. (PMID: 19258295) Methods. 2015 May;77-78:92-103. (PMID: 25312582) Biochim Biophys Acta. 2001 May 28;1519(1-2):55-64. (PMID: 11406271) BMC Immunol. 2006;7:7. (PMID: 16603068) Nature. 2013 Sep 12;501(7466):217-21. (PMID: 23934111) J Med Chem. 2006 Jun 29;49(13):3926-32. (PMID: 16789748) World J Gastroenterol. 2013 Jun 7;19(21):3249-54. (PMID: 23745026) Nature. 2012 Nov 1;491(7422):56-65. (PMID: 23128226) Nat Rev Cancer. 2011 May;11(5):364-74. (PMID: 21508973) Comp Med. 2004 Oct;54(5):533-42. (PMID: 15575367) Biochem J. 1984 Oct 1;223(1):47-51. (PMID: 6093770) Invest Ophthalmol Vis Sci. 2014 Feb;55(2):688-95. (PMID: 24408986) Science. 2015 Jan 23;347(6220):1260419. (PMID: 25613900) PLoS One. 2011;6(7):e22181. (PMID: 21765948) Mol Cell Proteomics. 2005 Dec;4(12):1920-32. (PMID: 16127175) Nucleic Acids Res. 2003 Jul 1;31(13):3375-80. (PMID: 12824331) PLoS One. 2014;9(1):e85850. (PMID: 24409334) Nat Genet. 2000 Oct;26(2):163-75. (PMID: 11017071) J Biol Chem. 2001 Jul 27;276(30):28525-31. (PMID: 11375982) J Neurosci. 2009 Jul 22;29(29):9330-43. (PMID: 19625523) Invest Ophthalmol Vis Sci. 2006 Dec;47(12):5469-75. (PMID: 17122138) Brain Res Bull. 2009 Mar 16;78(4-5):131. (PMID: 19056471) PLoS One. 2012;7(3):e33266. (PMID: 22432009) Comp Biochem Physiol B Biochem Mol Biol. 2002 Feb;131(2):221-5. (PMID: 11818243) Cell Biochem Biophys. 2013 Jun;66(2):371-7. (PMID: 23212179) CNS Neurol Disord Drug Targets. 2008 Jun;7(3):313-20. (PMID: 18673215) Genome Res. 2010 Sep;20(9):1297-303. (PMID: 20644199) Neuron. 2013 Dec 4;80(5):1175-89. (PMID: 24210906) Hum Mol Genet. 2015 Aug 15;24(16):4584-98. (PMID: 25994508) Exp Eye Res. 1985 Jul;41(1):97-103. (PMID: 2993010) Mech Dev. 1999 Dec;89(1-2):201-9. (PMID: 10559499) J Biol Chem. 2010 Mar 19;285(12):8808-23. (PMID: 20086007) J Biol Chem. 2014 Jul 11;289(28):19383-94. (PMID: 24838245) Genome Biol. 2009;10(11):R130. (PMID: 19919682) Cell. 1995 Apr 7;81(1):27-40. (PMID: 7720071) PLoS One. 2015;10(4):e0122352. (PMID: 25856303) Biochim Biophys Acta. 2011 Apr;1812(4):549-57. (PMID: 20804843) N Engl J Med. 2005 Jun 9;352(23):2413-23. (PMID: 15944426) Am J Pathol. 2012 Aug;181(2):388-91. (PMID: 22733009) Genomics. 1998 Feb 15;48(1):117-20. (PMID: 9503024) Curr Eye Res. 2000 Sep;21(3):710-20. (PMID: 11120559) Am J Hum Genet. 2014 Oct 2;95(4):360-70. (PMID: 25262651) J Pharmacol Exp Ther. 2010 Dec;335(3):645-52. (PMID: 20823194) Hum Mol Genet. 2014 May 15;23(10):2665-77. (PMID: 24381307) Seizure. 2011 Jun;20(5):395-401. (PMID: 21315622) J Neurosci Res. 2012 Apr;90(4):802-15. (PMID: 22065590) J Neurochem. 2006 Feb;96(3):802-14. (PMID: 16405498) J Neurochem. 2008 May;105(3):666-76. (PMID: 18088374) Invest Ophthalmol Vis Sci. 2010 Jun;51(6):3049-54. (PMID: 20107181) Neuroscience. 1976 Jun;1(3):159-65. (PMID: 11370226) J Cell Sci. 1993 Jul;105 ( Pt 3):787-98. (PMID: 8408304) Fly (Austin). 2012 Apr-Jun;6(2):80-92. (PMID: 22728672) Invest Ophthalmol Vis Sci. 2011 Sep;52(10):7059-67. (PMID: 21757584) PLoS Genet. 2012;8(10):e1003001. (PMID: 23055945) Trends Neurosci. 2015 Aug;38(8):468-74. (PMID: 26187720) Semin Arthritis Rheum. 2003 Jun;32(6):345-69. (PMID: 12833244) FASEB J. 2012 Mar;26(3):1204-17. (PMID: 22173972) Nature. 2008 Nov 20;456(7220):409-12. (PMID: 19020623) Acta Biochim Biophys Sin (Shanghai). 2005 Nov;37(11):719-27. (PMID: 16270150) Curr Eye Res. 2007 Mar;32(3):259-69. (PMID: 17453946) Mol Neurobiol. 2006 Jun;33(3):215-36. (PMID: 16954597) Biochem J. 2012 Nov 1;447(3):335-51. (PMID: 23035980) Curr Eye Res. 2014 Apr;39(4):421-4. (PMID: 24215535) Brain Inj. 2014;28(2):133-7. (PMID: 24456052) Pathobiology. 2015 Sep;82(3-4):133-41. (PMID: 26330354) Br J Cancer. 2004 Jul 19;91(2):355-8. (PMID: 15188009) J Diabetes Complications. 2013 Jan-Feb;27(1):54-8. (PMID: 23021796) Surv Ophthalmol. 2008 Mar-Apr;53(2):150-63. (PMID: 18348880) Genomics. 1997 Oct 1;45(1):175-84. (PMID: 9339374) Ophthalmology. 1990 Sep;97(9):1125-35; discussion 1135-6. (PMID: 2234842) Curr Protoc Protein Sci. 2007 Nov;Chapter 2:Unit 2.9. (PMID: 18429317) |
معلومات مُعتمدة: | F31 EY026789 United States EY NEI NIH HHS; R21 AG050437 United States AG NIA NIH HHS; R01 EY024665 United States EY NEI NIH HHS; K08 EY020530 United States EY NEI NIH HHS; R01 EY024698 United States EY NEI NIH HHS; R01 EY020542 United States EY NEI NIH HHS; R01 EY025225 United States EY NEI NIH HHS; F30 EY027986 United States EY NEI NIH HHS; T32 GM007337 United States GM NIGMS NIH HHS; T32 GM008629 United States GM NIGMS NIH HHS |
المشرفين على المادة: | 0 (RNA, Neoplasm) EC 3.4.22.- (Calpain) EC 3.4.22.- (Capn5 protein, human) |
تواريخ الأحداث: | Date Created: 20160507 Date Completed: 20170615 Latest Revision: 20210624 |
رمز التحديث: | 20240628 |
مُعرف محوري في PubMed: | PMC4868102 |
DOI: | 10.1167/iovs.15-18680 |
PMID: | 27152965 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1552-5783 |
---|---|
DOI: | 10.1167/iovs.15-18680 |