دورية أكاديمية
Dynamics of Translation of Single mRNA Molecules In Vivo.
| العنوان: | Dynamics of Translation of Single mRNA Molecules In Vivo. |
|---|---|
| المؤلفون: | Yan X; Department of Cellular and Molecular Pharmacology, Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, CA 94158-2517, USA., Hoek TA; Hubrecht Institute, The Royal Netherlands Academy of Arts and Sciences (KNAW) and University Medical Center Utrecht, Utrecht 3584CT, the Netherlands., Vale RD; Department of Cellular and Molecular Pharmacology, Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, CA 94158-2517, USA., Tanenbaum ME; Hubrecht Institute, The Royal Netherlands Academy of Arts and Sciences (KNAW) and University Medical Center Utrecht, Utrecht 3584CT, the Netherlands. Electronic address: m.tanenbaum@hubrecht.eu. |
| المصدر: | Cell [Cell] 2016 May 05; Vol. 165 (4), pp. 976-89. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Cell Press Country of Publication: United States NLM ID: 0413066 Publication Model: Print Cited Medium: Internet ISSN: 1097-4172 (Electronic) Linking ISSN: 00928674 NLM ISO Abbreviation: Cell Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Cambridge, Ma : Cell Press Original Publication: Cambridge, MIT Press. |
| مواضيع طبية MeSH: | Protein Biosynthesis*, Optical Imaging/*methods , RNA, Messenger/*metabolism, 5' Untranslated Regions ; Cell Cycle ; Cell Cycle Proteins/metabolism ; F-Box Proteins/metabolism ; Fluorescence ; Genes, Reporter ; Genetic Techniques ; Green Fluorescent Proteins/analysis ; Humans ; Luminescent Proteins/analysis ; Peptide Chain Elongation, Translational ; Peptide Chain Initiation, Translational ; RNA, Messenger/chemistry ; Ribosomes/metabolism ; Red Fluorescent Protein |
| مستخلص: | Regulation of mRNA translation, the process by which ribosomes decode mRNAs into polypeptides, is used to tune cellular protein levels. Currently, methods for observing the complete process of translation from single mRNAs in vivo are unavailable. Here, we report the long-term (>1 hr) imaging of single mRNAs undergoing hundreds of rounds of translation in live cells, enabling quantitative measurements of ribosome initiation, elongation, and stalling. This approach reveals a surprising heterogeneity in the translation of individual mRNAs within the same cell, including rapid and reversible transitions between a translating and non-translating state. Applying this method to the cell-cycle gene Emi1, we find strong overall repression of translation initiation by specific 5' UTR sequences, but individual mRNA molecules in the same cell can exhibit dramatically different translational efficiencies. The ability to observe translation of single mRNA molecules in live cells provides a powerful tool to study translation regulation. (Copyright © 2016 Elsevier Inc. All rights reserved.) |
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| معلومات مُعتمدة: | R01 GM038499 United States GM NIGMS NIH HHS |
| المشرفين على المادة: | 0 (5' Untranslated Regions) 0 (Cell Cycle Proteins) 0 (F-Box Proteins) 0 (FBXO5 protein, human) 0 (Luminescent Proteins) 0 (RNA, Messenger) 147336-22-9 (Green Fluorescent Proteins) |
| تواريخ الأحداث: | Date Created: 20160507 Date Completed: 20170110 Latest Revision: 20240325 |
| رمز التحديث: | 20240325 |
| مُعرف محوري في PubMed: | PMC4889334 |
| DOI: | 10.1016/j.cell.2016.04.034 |
| PMID: | 27153498 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1097-4172 |
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| DOI: | 10.1016/j.cell.2016.04.034 |