دورية أكاديمية
أعرض في EDS

The dsRNA Virus Papaya Meleira Virus and an ssRNA Virus Are Associated with Papaya Sticky Disease.

التفاصيل البيبلوغرافية
العنوان: The dsRNA Virus Papaya Meleira Virus and an ssRNA Virus Are Associated with Papaya Sticky Disease.
المؤلفون: Sá Antunes TF; Núcleo de Biotecnologia, Universidade Federal do Espírito Santo, Vitória, Espírito Santo, Brazil., Amaral RJ; Núcleo de Biotecnologia, Universidade Federal do Espírito Santo, Vitória, Espírito Santo, Brazil., Ventura JA; Núcleo de Biotecnologia, Universidade Federal do Espírito Santo, Vitória, Espírito Santo, Brazil.; Instituto Capixaba de Pesquisa, Assistência Técnica e Extensão Rural, Vitória, Espírito Santo, Brazil., Godinho MT; Dep. de Fitopatologia/BIOAGRO, Universidade Federal de Viçosa, 36570-900, Viçosa, Minas Gerais, Brazil., Amaral JG; Dep. de Fitopatologia/BIOAGRO, Universidade Federal de Viçosa, 36570-900, Viçosa, Minas Gerais, Brazil., Souza FO; Dep. de Microbiologia/BIOAGRO, Universidade Federal de Viçosa, 36570-900, Viçosa, Minas Gerais, Brazil., Zerbini PA; Dep. de Microbiologia/BIOAGRO, Universidade Federal de Viçosa, 36570-900, Viçosa, Minas Gerais, Brazil., Zerbini FM; Dep. de Fitopatologia/BIOAGRO, Universidade Federal de Viçosa, 36570-900, Viçosa, Minas Gerais, Brazil., Fernandes PM; Núcleo de Biotecnologia, Universidade Federal do Espírito Santo, Vitória, Espírito Santo, Brazil.
المصدر: PloS one [PLoS One] 2016 May 11; Vol. 11 (5), pp. e0155240. Date of Electronic Publication: 2016 May 11 (Print Publication: 2016).
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
أسماء مطبوعة: Original Publication: San Francisco, CA : Public Library of Science
مواضيع طبية MeSH: Genome, Viral* , Phylogeny*, Carica/*virology , Plant Diseases/*virology , Plant Viruses/*genetics , RNA Viruses/*genetics , Totiviridae/*genetics, Amino Acid Sequence ; Base Sequence ; Capsid Proteins/genetics ; Capsid Proteins/metabolism ; Gene Expression ; High-Throughput Nucleotide Sequencing ; Nucleic Acid Conformation ; Open Reading Frames ; Plant Viruses/classification ; Plant Viruses/ultrastructure ; RNA Viruses/classification ; RNA Viruses/ultrastructure ; RNA, Double-Stranded/chemistry ; RNA, Double-Stranded/genetics ; RNA, Double-Stranded/metabolism ; Sequence Alignment ; Sequence Homology, Amino Acid ; Totiviridae/classification ; Totiviridae/ultrastructure ; Virion/genetics ; Virion/ultrastructure
مستخلص: Papaya sticky disease, or "meleira", is one of the major diseases of papaya in Brazil and Mexico, capable of causing complete crop loss. The causal agent of sticky disease was identified as an isometric virus with a double stranded RNA (dsRNA) genome, named papaya meleira virus (PMeV). In the present study, PMeV dsRNA and a second RNA band of approximately 4.5 kb, both isolated from latex of papaya plants with severe symptoms of sticky disease, were deep-sequenced. The nearly complete sequence obtained for PMeV dsRNA is 8,814 nucleotides long and contains two putative ORFs; the predicted ORF1 and ORF2 display similarity to capsid proteins and RdRp's, respectively, from mycoviruses tentatively classified in the family Totiviridae. The sequence obtained for the second RNA is 4,515 nucleotides long and contains two putative ORFs. The predicted ORFs 1 and 2 display 48% and 73% sequence identity, respectively, with the corresponding proteins of papaya virus Q, an umbravirus recently described infecting papaya in Ecuador. Viral purification in a sucrose gradient allowed separation of particles containing each RNA. Mass spectrometry analysis indicated that both PMeV and the second RNA virus (named papaya meleira virus 2, PMeV2) were encapsidated in particles formed by the protein encoded by PMeV ORF1. The presence of both PMeV and PMeV2 was confirmed in field plants showing typical symptoms of sticky disease. Interestingly, PMeV was detected alone in asymptomatic plants. Together, our results indicate that sticky disease is associated with double infection by PMeV and PMeV2.
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المشرفين على المادة: 0 (Capsid Proteins)
0 (RNA, Double-Stranded)
تواريخ الأحداث: Date Created: 20160512 Date Completed: 20170706 Latest Revision: 20240327
رمز التحديث: 20240327
مُعرف محوري في PubMed: PMC4863961
DOI: 10.1371/journal.pone.0155240
PMID: 27166626
قاعدة البيانات: MEDLINE
الوصف
تدمد:1932-6203
DOI:10.1371/journal.pone.0155240