دورية أكاديمية
Vitamin D regulates the tight-junction protein expression in active ulcerative colitis.
| العنوان: | Vitamin D regulates the tight-junction protein expression in active ulcerative colitis. |
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| المؤلفون: | Stio M; a Department of Biomedical, Experimental and Clinical Sciences , 'Mario Serio' University of Florence , Florence , Italy ;, Retico L; b Gastroenterology Unit , Azienda Ospedaliero-Universitaria Careggi , Florence , Italy., Annese V; b Gastroenterology Unit , Azienda Ospedaliero-Universitaria Careggi , Florence , Italy., Bonanomi AG; b Gastroenterology Unit , Azienda Ospedaliero-Universitaria Careggi , Florence , Italy. |
| المصدر: | Scandinavian journal of gastroenterology [Scand J Gastroenterol] 2016 Oct; Vol. 51 (10), pp. 1193-9. Date of Electronic Publication: 2016 May 20. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Informa Healthcare Country of Publication: England NLM ID: 0060105 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1502-7708 (Electronic) Linking ISSN: 00365521 NLM ISO Abbreviation: Scand J Gastroenterol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: London : Informa Healthcare Original Publication: Oslo : Universitetsforlager |
| مواضيع طبية MeSH: | Claudin-2/*metabolism , Claudin-4/*metabolism , Claudins/*metabolism , Colitis, Ulcerative/*pathology , Vitamin D/*blood, Adult ; Biopsy ; Cells, Cultured ; Down-Regulation ; Epithelial Cells/metabolism ; Female ; Humans ; Interleukin-13/blood ; Interleukin-6/blood ; Intestinal Mucosa/pathology ; Male ; Middle Aged ; Up-Regulation |
| مستخلص: | Objective: Epithelial barrier function is primarily regulated by the tight-junction proteins. Ulcerative colitis (UC) is characterized by Th2 immune response with inflammation and epithelial barrier dysfunction, including an elevation of claudin-2 protein function. Recent studies support an important role of vitamin D in the pathogenesis as well as potential therapy of IBD. Vitamin D deficiency is in fact common in patients with IBD. The aim of the study was to determine whether vitamin D could affect IL-13 and IL-6 levels, and regulate the activity of tight-junction proteins. Claudin-1, -2, -4, and -7 in the inflamed and non-inflamed colonic mucosa of UC patients. Material and Methods: Biopsies from inflamed and non-inflamed tract of colon and rectum from the same active UC patients were cultured with1,25(OH)2D3. IL-13, IL-6 and the tight-junction proteins level were determined. Results: Claudin-1 and claudin-2 proteins were up-regulated in active UC. The treatment with 1,25(OH)2D3 decreases the claudin-1 and claudin-2 protein levels in both inflamed and non-inflamed tract. Claudin-4 and claudin-7 proteins were down-regulated and their levels increase after incubation with the 1,25(OH)2D3. When the biopsies were incubated with 1,25(OH)2D3, a decrease in IL-13 and IL-6 levels was registered. Conclusions: Our results, indicating the inhibition of cytokine levels and the regulation of claudin-2, claudin-4, and claudin-7 by 1,25(OH)2D3, suggest that vitamin D may represent a potential therapeutic agent for the treatment of active UC. |
| فهرسة مساهمة: | Keywords: Tight-junctions proteins; ulcerative colitis; vitamin D |
| المشرفين على المادة: | 0 (CLDN7 protein, human) 0 (Claudin-2) 0 (Claudin-4) 0 (Claudins) 0 (IL6 protein, human) 0 (Interleukin-13) 0 (Interleukin-6) 1406-16-2 (Vitamin D) |
| تواريخ الأحداث: | Date Created: 20160522 Date Completed: 20170606 Latest Revision: 20170817 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1080/00365521.2016.1185463 |
| PMID: | 27207502 |
| قاعدة البيانات: | MEDLINE |
PDF full text from Publisher | تدمد: | 1502-7708 |
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| DOI: | 10.1080/00365521.2016.1185463 |