دورية أكاديمية
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Comparative Analysis of the Antimicrobial Activities of Plant Defensin-Like and Ultrashort Peptides against Food-Spoiling Bacteria.

التفاصيل البيبلوغرافية
العنوان: Comparative Analysis of the Antimicrobial Activities of Plant Defensin-Like and Ultrashort Peptides against Food-Spoiling Bacteria.
المؤلفون: Kraszewska J; Microbiology Department, Moyne Institute, School of Genetics and Microbiology, Trinity College Dublin, Dublin, Ireland., Beckett MC; Microbiology Department, Moyne Institute, School of Genetics and Microbiology, Trinity College Dublin, Dublin, Ireland., James TC; Microbiology Department, Moyne Institute, School of Genetics and Microbiology, Trinity College Dublin, Dublin, Ireland., Bond U; Microbiology Department, Moyne Institute, School of Genetics and Microbiology, Trinity College Dublin, Dublin, Ireland ubond@tcd.ie.
المصدر: Applied and environmental microbiology [Appl Environ Microbiol] 2016 Jun 30; Vol. 82 (14), pp. 4288-4298. Date of Electronic Publication: 2016 Jun 30 (Print Publication: 2016).
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: American Society for Microbiology Country of Publication: United States NLM ID: 7605801 Publication Model: Electronic-Print Cited Medium: Internet ISSN: 1098-5336 (Electronic) Linking ISSN: 00992240 NLM ISO Abbreviation: Appl Environ Microbiol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Washington, American Society for Microbiology.
مواضيع طبية MeSH: Food Microbiology*, Anti-Bacterial Agents/*pharmacology , Bacteria/*drug effects , Defensins/*pharmacology , Oligopeptides/*pharmacology , Plant Proteins/*pharmacology , Plants/*chemistry, Computational Biology ; Defensins/genetics ; Defensins/isolation & purification ; Drug Discovery ; Drug Synergism ; Microbial Sensitivity Tests ; Oligopeptides/genetics ; Oligopeptides/isolation & purification ; Plant Proteins/genetics ; Plant Proteins/isolation & purification
مستخلص: Unlabelled: Antimicrobial peptides offer potential as novel therapeutics to combat food spoilage and poisoning caused by pathogenic and nonpathogenic bacteria. Our previous studies identified the peptide human beta-defensin 3 (HBD3) as a potent antimicrobial agent against a wide range of beer-spoiling bacteria. Thus, HBD3 is an excellent candidate for development as an additive to prevent food and beverage spoilage. To expand the repertoire of peptides with antimicrobial activity against bacteria associated with food spoilage and/or food poisoning, we carried out an in silico discovery pipeline to identify peptides with structure and activity similar to those of HBD3, focusing on peptides of plant origin. Using a standardized assay, we compared the antimicrobial activities of nine defensin-like plant peptides to the activity of HBD3. Only two of the peptides, fabatin-2 and Cp-thionin-2, displayed antimicrobial activity; however, the peptides differed from HBD3 in being sensitive to salt and were thermostable. We also compared the activities of several ultrashort peptides to that of HBD3. One of the peptides, the synthetic tetrapeptide O3TR, displayed biphasic antimicrobial activity but had a narrower host range than HBD3. Finally, to determine if the peptides might act in concert to improve antimicrobial activity, we compared the activities of the peptides in pairwise combinations. The plant defensin-like peptides fabatin-2 and Cp-thionin-2 displayed a synergistic effect with HBD3, while O3TR was antagonistic. Thus, some plant defensin-like peptides are effective antimicrobials and may act in concert with HBD3 to control bacteria associated with food spoilage and food poisoning.
Importance: Food spoilage and food poisoning caused by bacteria can have major health and economic implications for human society. With the rise in resistance to conventional antibiotics, there is a need to identify new antimicrobials to combat these outbreaks in our food supply. Here we screened plant peptide databases to identify peptides that share structural similarity with the human defensin peptide HBD3, which has known antimicrobial activity against food-spoiling bacteria. We show that two of the plant peptides display antimicrobial activity against bacteria associated with food spoilage. When combined with HBD3, the peptides are highly effective. We also analyzed the activity of an easily made ultrashort synthetic peptide, O3TR. We show that this small peptide also displays antimicrobial activity against food-spoiling bacteria but is not as effective as HBD3 or the plant peptides. The plant peptides identified are good candidates for development as natural additives to prevent food spoilage.
(Copyright © 2016, American Society for Microbiology. All Rights Reserved.)
References: Front Microbiol. 2014 Mar 20;5:97. (PMID: 24688483)
Nucleic Acids Res. 2007;35(18):6268-79. (PMID: 17855393)
Crit Rev Biotechnol. 2012 Jun;32(2):143-71. (PMID: 22074402)
Infect Immun. 2010 Jun;78(6):2793-800. (PMID: 20385753)
FASEB J. 2010 Sep;24(9):3232-8. (PMID: 20410445)
Curr Pharm Des. 2010;16(28):3204-11. (PMID: 20687884)
Biochem Soc Trans. 2006 Apr;34(Pt 2):251-6. (PMID: 16545087)
Curr Pharm Des. 2011 Dec;17(38):4254-69. (PMID: 22204426)
J Biol Chem. 2001 Feb 23;276(8):5707-13. (PMID: 11085990)
Bioorg Med Chem Lett. 2007 Aug 1;17(15):4343-6. (PMID: 17553680)
Immunol Rev. 2012 Jan;245(1):84-112. (PMID: 22168415)
Plant Physiol. 2000 Apr;122(4):1015-24. (PMID: 10759497)
Curr Protein Pept Sci. 2009 Dec;10(6):585-606. (PMID: 19751192)
Proc Natl Acad Sci U S A. 2014 Dec 30;111(52):18703-8. (PMID: 25512526)
Peptides. 2014 May;55:65-78. (PMID: 24548568)
Structure. 2013 Oct 8;21(10):1735-42. (PMID: 24035711)
Virulence. 2010 Sep-Oct;1(5):440-64. (PMID: 21178486)
Gut Microbes. 2010 Nov-Dec;1(6):401-6. (PMID: 21468224)
Nucleic Acids Res. 2016 Jan 4;44(D1):D1104-12. (PMID: 26578581)
Proc Natl Acad Sci U S A. 2014 Apr 8;111(14):E1409-18. (PMID: 24706874)
Phys Chem Chem Phys. 2012 Dec 5;14(45):15739-51. (PMID: 23093307)
Biochimie. 2007 May;89(5):605-12. (PMID: 17321030)
FEBS Lett. 1990 Sep 17;270(1-2):191-4. (PMID: 2226781)
Nucleic Acids Res. 2009 Jan;37(Database issue):D963-8. (PMID: 18836196)
Biochim Biophys Acta. 2004 Dec 15;1667(2):148-56. (PMID: 15581850)
J Mol Biol. 1998 May 29;279(1):257-70. (PMID: 9636715)
Toxicology. 1994 Feb 28;87(1-3):131-49. (PMID: 7512758)
Antimicrob Agents Chemother. 2009 Aug;53(8):3211-7. (PMID: 19470516)
FEBS J. 2009 Nov;276(22):6465-73. (PMID: 19817858)
Med Electron Microsc. 2003 Dec;36(4):247-52. (PMID: 16228657)
Proc Natl Acad Sci U S A. 1999 Aug 3;96(16):8913-8. (PMID: 10430870)
Helicobacter. 2013 Feb;18(1):6-12. (PMID: 23067102)
J Appl Microbiol. 2014 Feb;116(2):368-79. (PMID: 24176036)
Immunogenetics. 2007 Jul;59(7):573-80. (PMID: 17483936)
J Pept Sci. 2008 Apr;14(4):518-23. (PMID: 18085509)
J Antimicrob Chemother. 2015 May;70(5):1285-9. (PMID: 25681127)
Meded Rijksuniv Gent Fak Landbouwkd Toegep Biol Wet. 2002;67(2):327-31. (PMID: 12701440)
FEMS Microbiol Lett. 1997 Apr 1;149(1):59-64. (PMID: 9103978)
Curr Pharm Des. 2011 Dec;17(38):4270-93. (PMID: 22204427)
Chem Biol Drug Des. 2010 Jun;75(6):563-9. (PMID: 20374251)
Immunogenetics. 2005 Apr;57(1-2):90-8. (PMID: 15744537)
Chem Phys Lipids. 2012 Apr;165(3):282-301. (PMID: 22366307)
Cancer Res. 2010 Jan 15;70(2):440-6. (PMID: 20068163)
Int J Antimicrob Agents. 2004 Dec;24(6):536-47. (PMID: 15555874)
FEBS J. 2006 Aug;273(15):3489-97. (PMID: 16824043)
Nucleic Acids Res. 2014 Jan;42(Database issue):D1154-8. (PMID: 24265220)
Angew Chem Int Ed Engl. 2014 Sep 26;53(40):10612-23. (PMID: 25079086)
J Biol Chem. 1992 Aug 5;267(22):15301-9. (PMID: 1639777)
J Agric Food Chem. 2011 Feb 23;59(4):1145-51. (PMID: 21268582)
Pharmaceuticals (Basel). 2015 Nov 16;8(4):711-57. (PMID: 26580629)
Nucleic Acids Res. 2007 Jan;35(Database issue):D265-8. (PMID: 17090586)
Proteins. 2007 Aug 1;68(2):530-40. (PMID: 17444520)
Pharmaceuticals (Basel). 2015 Mar 23;8(1):123-50. (PMID: 25806720)
Curr Pharm Des. 2007;13(30):3096-118. (PMID: 17979752)
Int J Med Microbiol. 2008 Oct;298(7-8):619-33. (PMID: 18455476)
Biofouling. 2015;31(2):193-9. (PMID: 25760404)
Nat Rev Microbiol. 2005 Mar;3(3):238-50. (PMID: 15703760)
Proteins. 2009;77 Suppl 9:89-99. (PMID: 19701941)
المشرفين على المادة: 0 (Anti-Bacterial Agents)
0 (Defensins)
0 (Oligopeptides)
0 (Plant Proteins)
تواريخ الأحداث: Date Created: 20160522 Date Completed: 20171002 Latest Revision: 20181113
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC4959217
DOI: 10.1128/AEM.00558-16
PMID: 27208129
قاعدة البيانات: MEDLINE
الوصف
تدمد:1098-5336
DOI:10.1128/AEM.00558-16