دورية أكاديمية
Bone induction at physiological doses of BMP through localization by clay nanoparticle gels.
| العنوان: | Bone induction at physiological doses of BMP through localization by clay nanoparticle gels. |
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| المؤلفون: | Gibbs DM; Bone and Joint Research Group, Centre for Human Development, Stem Cells & Regeneration, Institute of Developmental Sciences, University of Southampton, Southampton, SO16 6YD, UK., Black CR; Bone and Joint Research Group, Centre for Human Development, Stem Cells & Regeneration, Institute of Developmental Sciences, University of Southampton, Southampton, SO16 6YD, UK., Hulsart-Billstrom G; Bone and Joint Research Group, Centre for Human Development, Stem Cells & Regeneration, Institute of Developmental Sciences, University of Southampton, Southampton, SO16 6YD, UK., Shi P; Bone and Joint Research Group, Centre for Human Development, Stem Cells & Regeneration, Institute of Developmental Sciences, University of Southampton, Southampton, SO16 6YD, UK., Scarpa E; Bone and Joint Research Group, Centre for Human Development, Stem Cells & Regeneration, Institute of Developmental Sciences, University of Southampton, Southampton, SO16 6YD, UK., Oreffo RO; Bone and Joint Research Group, Centre for Human Development, Stem Cells & Regeneration, Institute of Developmental Sciences, University of Southampton, Southampton, SO16 6YD, UK. Electronic address: Richard.Oreffo@soton.ac.uk., Dawson JI; Bone and Joint Research Group, Centre for Human Development, Stem Cells & Regeneration, Institute of Developmental Sciences, University of Southampton, Southampton, SO16 6YD, UK. Electronic address: jid@soton.ac.uk. |
| المصدر: | Biomaterials [Biomaterials] 2016 Aug; Vol. 99, pp. 16-23. Date of Electronic Publication: 2016 May 10. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Science Country of Publication: Netherlands NLM ID: 8100316 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1878-5905 (Electronic) Linking ISSN: 01429612 NLM ISO Abbreviation: Biomaterials Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <1995-> : Amsterdam : Elsevier Science Original Publication: [Guilford, England] : IPC Science and Technology Press, 1980- |
| مواضيع طبية MeSH: | Bone Morphogenetic Protein 2/*administration & dosage , Bone Morphogenetic Protein 2/*chemistry , Bone and Bones/*drug effects , Nanoparticles/*chemistry , Osteogenesis/*drug effects , Silicates/*chemistry, Animals ; Bone and Bones/cytology ; Bone and Bones/physiology ; Cell Differentiation ; Cell Line ; Drug Delivery Systems ; Drug Liberation ; Gels ; Humans ; Mice ; Stromal Cells/cytology ; Stromal Cells/drug effects |
| مستخلص: | Bone Morphogenic Protein 2 (BMP2) can induce ectopic bone. This ability, which first motivated the widespread application of BMP2 in fracture healing and spinal arthrodesis has, more recently, been indicated as one of several serious adverse effects associated with the supra-physiological doses of BMP2 relied upon for clinical efficacy. Key to harnessing BMPs and other agents safely and effectively will be the ability to localize activity at a target site at substantially reduced doses. Clay (Laponite) nanoparticles can self assemble into gels under physiological conditions and bind growth factors for enhanced and localized efficacy. Here we show the ability to localize and enhance the activity of BMP2 to achieve ectopic bone formation at doses within the sub-microgram per ml range of concentrations sufficient to induce differentiation of responsive cell populations in vitro and at approximately 3000 fold lower than those employed in clinical practice. (Copyright © 2016 Elsevier Ltd. All rights reserved.) |
| معلومات مُعتمدة: | BB/G010579/1 United Kingdom BB_ Biotechnology and Biological Sciences Research Council; MR/L012626/1 United Kingdom MRC_ Medical Research Council |
| فهرسة مساهمة: | Keywords: Bone induction; Bone morphogenic protein; Clay gels; Drug delivery; Hydrogels; Smectites |
| المشرفين على المادة: | 0 (BMP2 protein, human) 0 (Bone Morphogenetic Protein 2) 0 (Gels) 0 (Silicates) D703131383 (laponite) |
| تواريخ الأحداث: | Date Created: 20160523 Date Completed: 20180222 Latest Revision: 20220311 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1016/j.biomaterials.2016.05.010 |
| PMID: | 27209259 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1878-5905 |
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| DOI: | 10.1016/j.biomaterials.2016.05.010 |