دورية أكاديمية
Quantitative in vivo optical tomography of cancer progression & vasculature development in adult zebrafish.
| العنوان: | Quantitative in vivo optical tomography of cancer progression & vasculature development in adult zebrafish. |
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| المؤلفون: | Kumar S; Department of Physics, Imperial College London, London SW7 2AZ, UK., Lockwood N; Division of Medicine, University College London, London WC1E 6JF, UK.; CoMPLEX, University College London, London WC1E 6BT, UK., Ramel MC; Division of Medicine, University College London, London WC1E 6JF, UK.; Department of Life Sciences, Imperial College London, London SW7 2AZ, UK., Correia T; Department of Computer Science, University College London, London WC1E 6BT, UK., Ellis M; Department of Neurodegenerative Disease, UCL Institute of Neurology, London WC1 3BG, UK., Alexandrov Y; Department of Physics, Imperial College London, London SW7 2AZ, UK., Andrews N; Department of Life Sciences, Imperial College London, London SW7 2AZ, UK.; Institute of Chemical Biology, Department of Chemistry, Imperial College, London SW7 2AZ, UK., Patel R; Division of Medicine, University College London, London WC1E 6JF, UK., Bugeon L; Department of Life Sciences, Imperial College London, London SW7 2AZ, UK., Dallman MJ; Department of Life Sciences, Imperial College London, London SW7 2AZ, UK., Brandner S; Department of Neurodegenerative Disease, UCL Institute of Neurology, London WC1 3BG, UK.; Division of Neuropathology, The National Hospital for Neurology and Neurosurgery, University College London NHS Foundation Trust, London WC1N 3BG, UK., Arridge S; Department of Computer Science, University College London, London WC1E 6BT, UK., Katan M; Division of Structural and Molecular Biology, University College London, London WC1E 6BT, UK., McGinty J; Department of Physics, Imperial College London, London SW7 2AZ, UK., Frankel P; Division of Medicine, University College London, London WC1E 6JF, UK., French PM; Department of Physics, Imperial College London, London SW7 2AZ, UK. |
| المصدر: | Oncotarget [Oncotarget] 2016 Jul 12; Vol. 7 (28), pp. 43939-43948. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Impact Journals Country of Publication: United States NLM ID: 101532965 Publication Model: Print Cited Medium: Internet ISSN: 1949-2553 (Electronic) Linking ISSN: 19492553 NLM ISO Abbreviation: Oncotarget Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Albany, N.Y. : Impact Journals |
| مواضيع طبية MeSH: | Disease Models, Animal*, Imaging, Three-Dimensional/*methods , Liver Neoplasms/*pathology , Neovascularization, Pathologic/*pathology , Tomography, Optical/*methods, Animals ; Animals, Genetically Modified ; Disease Progression ; Zebrafish |
| مستخلص: | We describe a novel approach to study tumour progression and vasculature development in vivo via global 3-D fluorescence imaging of live non-pigmented adult zebrafish utilising angularly multiplexed optical projection tomography with compressive sensing (CS-OPT). This "mesoscopic" imaging method bridges a gap between established ~μm resolution 3-D fluorescence microscopy techniques and ~mm-resolved whole body planar imaging and diffuse tomography. Implementing angular multiplexing with CS-OPT, we demonstrate the in vivo global imaging of an inducible fluorescently labelled genetic model of liver cancer in adult non-pigmented zebrafish that also present fluorescently labelled vasculature. In this disease model, addition of a chemical inducer (doxycycline) drives expression of eGFP tagged oncogenic K-RASV12 in the liver of immune competent animals. We show that our novel in vivo global imaging methodology enables non-invasive quantitative imaging of the development of tumour and vasculature throughout the progression of the disease, which we have validated against established methods of pathology including immunohistochemistry. We have also demonstrated its potential for longitudinal imaging through a study of vascular development in the same zebrafish from early embryo to adulthood. We believe that this instrument, together with its associated analysis and data management tools, constitute a new platform for in vivo cancer studies and drug discovery in zebrafish disease models. Competing Interests: No potential conflicts of interest were disclosed. |
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| معلومات مُعتمدة: | 20256 United Kingdom CRUK_ Cancer Research UK; MR/K011561/1 United Kingdom MRC_ Medical Research Council; NC/C012107/1 United Kingdom NC3RS_ National Centre for the Replacement, Refinement and Reduction of Animals in Research |
| فهرسة مساهمة: | Keywords: KRas; adult zebrafish; cancer; hepatocellular carcinoma; optical projection tomography |
| تواريخ الأحداث: | Date Created: 20160604 Date Completed: 20180117 Latest Revision: 20240210 |
| رمز التحديث: | 20240210 |
| مُعرف محوري في PubMed: | PMC5190069 |
| DOI: | 10.18632/oncotarget.9756 |
| PMID: | 27259259 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1949-2553 |
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| DOI: | 10.18632/oncotarget.9756 |